Results 281 to 290 of about 568,309 (314)

Expression, Solubilization, and Purification of Bacterial Membrane Proteins

Current Protocols in Protein Science, 2016
AbstractBacterial integral membrane proteins play many important roles, including sensing changes in the environment, transporting molecules into and out of the cell, and in the case of commensal or pathogenic bacteria, interacting with the host organism.
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Prokaryotic Expression and Affinity Purification of DDX3 Protein

Protein & Peptide Letters
Background: DDX3 is a protein with RNA helicase activity that is involved in a variety of biological processes, and it is an important protein target for the development of broad-spectrum antiviral drugs, multiple cancers and chronic inflammation. Objective: The objective of this study is to establish a simple and efficient method to express and ...
Lan, Huang, Yue, Liang, Huijin, Hou, Min, Tang, Xinpeng, Liu, Yan-Ni, Ma, Shufang, Liang   +6 more
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[Expression and purification of EBV-LMP2 protein].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, 2011
To obtain a second Epstein-Barr virus membrane protein (LMP2) in insect cells.The full length EBV-LMP2 gene was inserted into baculovirus expression transfer vector pFastBac HT B to obtain the recombinant baculoviruses Bac-LMP2. And generation of recombinant baculoviruses was followed by transfection of the recombinant Bac-LMP2 into insect cells, then ...
Zhan, Wang, Jing, Ma, Ling, Zhou, Xiao-Guang, Zhang, Hai-Jun, Du, Song-Mei, Yang, Yi, Zeng   +6 more
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Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins

2011
Integral outer membrane proteins (OMPs) play key roles in solute transport, adhesion, and other processes. In Neisseria, they can also function as major protective antigens. Structural, biophysical, and immunological studies of Neisserial OMPs require their isolation in milligram quantities. Purification of any OMP directly from Neisseria would require
Saleem, Muhammad, Moore, Jeremy, Derrick, Jeremy P.   +2 more
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Expression and Purification of Pharmaceutical Proteins in Plants

Biological Engineering, 2008
The majority of human pharmaceuticals including vaccine and therapeutic proteins are currently produced in mammalian and microbial cell cultures. However, these technology platforms cannot meet the increasing consumer demand due to requirements of heavy upfront capital investment and their limited scalability.
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Expression and purification of amyloid β-protein, tau, and α-synuclein in Escherichia coli: a review

Critical Reviews in Biotechnology, 2020
Longgang Jia, Wenping Zhao, Wenjuan Wang   +2 more
exaly  

Purification of Expressed Proteins from Inclusion Bodies

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
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Isolation, purification, gene cloning and expression of antifungal protein from Bacillus amyloliquefaciens MG-3

Food Chemistry, 2021
Fen Yan, Hetong Lin
exaly  

Protein Expression, Purification, and Analysis

1999
Walt Ream, Katharine G. Field
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HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification

Protein Expression and Purification, 2009
Rachel Friedman Ohana, Lance P Encell, Michael R Slater   +2 more
exaly