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Expression and Purification of GST Fusion Proteins
Current Protocols in Protein Science, 1997AbstractThis unit describes the use of the glutathione‐S‐transferase (GST) gene fusion system as a method for high‐level protein expression and purification from bacterial lysates. Several pGEX vectors are available with multiple cloning sites to allow for unidirectional insertion of the coding‐region DNA into the pGEX vector. The GST fusion protein is
Sandra, Harper, David W, Speicher
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Expression and Purification of Membrane Proteins
2014Approximately 30% of a genome encodes for membrane proteins. They are one of the most important classes of proteins in that they can receive, differentiate, and transmit intra- and intercellular signals. Some examples of classes of membrane proteins include cell-adhesion molecules, translocases, and receptors in signaling pathways.
Jan, Kubicek +4 more
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Cloning technologies for protein expression and purification
Current Opinion in Biotechnology, 2006Detailed knowledge of the biochemistry and structure of individual proteins is fundamental to biomedical research. To further our understanding, however, proteins need to be purified in sufficient quantities, usually from recombinant sources. Although the sequences of genomes are now produced in automated factories purified proteins are not, because ...
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Expression, purification and properties of multidrug efflux proteins
Biochemical Society Transactions, 2000A general strategy is described for the amplified expression, purification and characterization in Escherichia coli of multidrug efflux proteins from Staphylococcus aureus, Bacillus subtilis, Methanococcus janaschii and E. coli. They all catalyse drug/H+ antiport of substrates such as quinolones and ethidium and exemplify a family of putatively 12 ...
Henderson, PJF, Hoyle, CK, Ward, A
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G-Protein-Coupled Receptor Expression and Purification
2014G-protein-coupled receptors (GPCRs) are integral proteins of the cell membrane and are directly involved in the regulation of many biological functions and in drug targeting. However, our knowledge of GPCRs' structure and function remains limited. The first bottleneck in GPCR studies is producing sufficient quantities of soluble, functional, and stable
Karolina, Corin +2 more
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Expression and purification of recombinant human EGFL7 protein
Protein Expression and Purification, 2009The secreted epidermal growth factor-like protein 7 (EGFL7) plays an important role in angiogenesis, especially in the recruitment of endothelial and smooth muscle cells to the site of the nascent vessel and their ordered assembly into functional vasculature.
Srdjan, Picuric +2 more
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Expression, purification and characterization of murine Dkk1 protein
Protein Expression and Purification, 2008Dickkopf-1 (Dkk1) protein is a secreted inhibitor of canonical Wnt signaling and modulates that pathway during embryonic development. It is also implicated in several diseases and hence Dkk1 is a potential target for therapeutic intervention. In the present study 6His-tagged Dkk1 expression and secretion was assessed in five mammalian cell types.
Damien, Fleury +6 more
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ADAMTS7: Recombinant Protein Expression and Purification
2019ADAMTS7 is a secreted protease that is predominantly expressed in tissues of the cardiovascular system and tendon. Although recent evidence suggests that it may play a role in the etiology of coronary artery disease, its physiological function and substrates are unknown.
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Expression and Purification of Maltose‐Binding Protein Fusions
Current Protocols in Molecular Biology, 1994AbstractThis unit describes the procedure for subcloning the sequence encoding the protein of interest into an maltose‐binding protein (MBP) vector, and expressing and purifying the fusion protein from the cytoplasm. MBP vectors include a sequence that encodes the four‐amino‐acid recognition site for the specific protease factor Xa.
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Expression, Purification, and Biophysical Studies of Chromodomain Proteins
2003Publisher Summary This chapter describes the structural features of chromodomains and mentions how this information can be used to identify chromodomains from amino acid sequences. The chapter employs various biophysical techniques to study the chromodomain and chromo shadow domains of HP1β.
Peter R, Nielsen +4 more
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