Results 31 to 40 of about 568,309 (314)
, 2008 Dps, found in many eubacterial and archaebacterial species, appears to protect cells from oxidative stress and/or nutrient-limited environment. Dps has been shown to accumulate during the stationary phase, to bind to DNA non-specifically, and to form a ...
MENDES, Guilherme Martines Teixeira +4 more
core +2 more sources
, 2010 Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV +11 more
core +1 more source
STAR Protocols, 2023 Summary: Protein-protein interactions play important roles in regulating cellular functions. We present an optimized disulfide cross-linking protocol for testing predicted interactions of soluble or membrane proteins. Coexpression in E.
Sandra Olenic, Lee Kroos
doaj +1 more source
Expression and purification of full-length anti-apoptotic Bcl-2 using cell-free protein synthesis
, 2011 The anti-apoptotic B cell CLL/lymphoma-2 (Bcl-2) protein is a key player in the regulation of programmed cell death and is linked to various types of cancer and their resistance to drug treatment.
Pedersen, Anders, +9 more
core +1 more source
Improvements of a high-throughput protein purification process using a calcium-dependent setup
, 2020 The Human Secretome Project aims to produce and purify all human secreted proteins as full-length. In order to enable this, a robust, gentle and effective purification process is needed, where multiple proteins can be purified in parallel.
Hanna Tegel +11 more
core +1 more source
, 2022 S.46-55Cardiovascular diseases are a prevalent cause of morbidity and mortality especially in industrialized countries. The human phosphatase and actin regulator 1 (PHACTR1) may be involved in such diseases, but its precise regulatory function remains ...
Gengenbach, B.B. +2 more
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Expression and purification of fused kinase from insect cells for in vitro kinase assay
STAR Protocols, 2021 Summary: The Fused (Fu) kinase is a key transducer of Hedgehog signaling, but its relevant substrates have remained obscured due to the difficulty of obtaining active Fu for in vitro kinase assay.
Yuhong Han, Jin Jiang
doaj +1 more source
Yeast ferrochelatase: Expression in a baculovirus system and purification of the expression protein [PDF]
Protein Science, 1992 AbstractThe terminal step of the heme biosynthetic pathway is catalyzed by the enzyme ferrochelatase (EC 4.99.1.1). In eukaryotes this enzyme is bound to the inner mitochondrial membrane with its active site facing the matrix side of the membrane. Previously this laboratory has characterized this enzyme via kinetic and protein chemical modification ...
M G, Eldridge, H A, Dailey
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High-yield expression and purification of a monotopic membrane glycosyltransferase
, 2009 Membrane proteins are essential to many cellular processes. However, the systematic study of membrane protein structure has been hindered by the difficulty in obtaining large quantities of these proteins.
Eriksson, Hanna M +6 more
core +1 more source
Expression, purification and characterization of Mycobacterium tuberculosis RpfE protein [PDF]
Journal of Biomedical Research, 2012 Resuscitation promoting factor E (RpfE) is one of the five Rpf-like proteins in Mycobacterium tuberculosis (M. tuberculosis). These Rpf-like proteins are secretory, which make them candidates for recognition by the host immune system. In this study, the RpfE gene was amplified from M. tuberculosis, cloned into the expression vectors pDE22 and pPRO EXHT,
Xue, Ying +6 more
openaire +2 more sources