Results 21 to 30 of about 568,309 (314)
High-throughput expression and purification of membrane proteins [PDF]
Journal of Structural Biology, 2010 High-throughput (HT) methodologies have had a tremendous impact on structural biology of soluble proteins. High-resolution structure determination relies on the ability of the macromolecule to form ordered crystals that diffract X-rays. While crystallization remains somewhat empirical, for a given protein, success is proportional to the number of ...
Filippo, Mancia, James, Love
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Proper chromosome alignment depends on BRCA2 phosphorylation by PLK1
Nature Communications, 2020 The BRCA2 tumour suppressor protein is known to play an important role in homologous recombination. Here the authors reveal how the phosphorylation of BRCA2 by Polo-like kinase 1 (PLK1) contributes to the regulation of mitosis.
Åsa Ehlén +12 more
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Transient transfection and purification of SARS-CoV-2 spike protein from mammalian cells
STAR Protocols, 2022 Summary: SARS-CoV-2 spike (S) protein ectodomain purification can be challenging, with engineered and natural variations often resulting in lower yields.
Victoria Stalls +2 more
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Scientific Reports, 2017 The quantity of milk and milk fat and proteins are particularly important traits in dairy livestock. However, little is known about the regions of the genome that influence these traits in goats.
Pauline Martin +11 more
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Filter trapping protocol to detect aggregated proteins in human cell lines
STAR Protocols, 2022 Summary: The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter-trapping assay protocol to detect global aggregated proteins in human cell lines ...
Jagat K. Chhipi-Shrestha +2 more
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STAR Protocols, 2022 Summary: LPR1 (LOW PHOSPHATE ROOT 1), a bacterial-type plant ferroxidase, is crucial for local root phosphate (Pi) sensing. Here, we present a detailed protocol for native (tag-free) protein purification of LPR1 from leaf extracts by differential ...
Nancy Tang, Christin Naumann
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Expression and purification of SARS coronavirus membrane protein [PDF]
Current Medical Science, 2004 To construct a recombinant plasmid Pet23a-M, the gene encoding severe acute respiratory syndrome (SARS) coronavirus membrane protein was amplified by RT-PCR and cloned into the expression plasmid Pet23a. Results of restriction endonuclease analysis, PCR detection and DNA sequencing analysis revealed that the cloned DNA sequence was the same as that ...
Wuxing, Dai +9 more
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Scientific Reports, 2018 A correction to this article has been published and is linked from the HTML and PDF versions of this paper.
Pauline Martin +11 more
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STAR Protocols, 2021 Summary: Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by the polyglutamine (polyQ) expansion in huntingtin (HTT) protein.
Hyeongju Kim +4 more
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Expression, purification and initial characterization of human meprin v from Pichia pastoris
, 2022 S.75-81Human meprin v (h-meprin v), a single-zinc metalloendoprotease of the astacin family, is potentially involved in disorders such as fibrosis and Alzheimer's disease. Here, we describe the expression of the enzyme in the yeast Pichia pastoris. The N-
Ramsbeck, Daniel +4 more
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