Results 1 to 10 of about 550,471 (169)

Protocol for expressing and purifying recombinant full-length Tau by combining affinity chromatography with preparative HPLC [PDF]

STAR Protocols
Summary: Deposits of microtubule-associated protein full-length Tau (Tau2N4R) are implicated as a hallmark of Alzheimer’s disease. Its biochemical and structural characterization is key to understanding disease progression, aggregate toxicity, and ...
Ruiheng Jing, Sayanta Mahapatra, Abhishek Bimali, Morgane Herlory, Maciej A. Walczak   +4 more
doaj   +2 more sources

Protein Expression and Purification [PDF]

Klinicka onkologie, 2014
Production of recombinant proteins is essential for many applications in both basic research and also in medicine, where recombinant proteins are used as pharmaceuticals. This review summarizes procedures involved in recombinant protein expression and purification, including molecular cloning of target genes into expression vectors, selection of the ...
E, Růčková, P, Müller, B, Vojtěšek
openaire   +2 more sources

On-bead purification and nanodisc reconstitution of human chemokine receptor complexes for structural and biophysical studies

STAR Protocols, 2023
Summary: Chemokine receptors, a subfamily of G-protein-coupled receptors (GPCRs), are responsible for cell migration during physiological processes as well as in diseases like inflammation and cancers.
Siyi Gu, Mian Huang, Tracy M. Handel
doaj   +1 more source

Quality control of protein reagents for the improvement of research data reproducibility

Nature Communications, 2021
Proteins and peptides are amongst the most widely used research reagents but often their quality is inadequate and can result in poor data reproducibility. Here we propose a simple set of guidelines that, when correctly applied to protein reagents should
Ario de Marco, Nick Berrow, Mario Lebendiker, Maria Garcia-Alai, Stefan H. Knauer, Blanca Lopez-Mendez, André Matagne, Annabel Parret, Kim Remans, Stephan Uebel, Bertrand Raynal   +10 more
doaj   +1 more source

Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples

STAR Protocols, 2021
Summary: The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the ...
Alex Steimle, Erica T. Grant, Mahesh S. Desai   +2 more
doaj   +1 more source

Protein abundance and folding rather than the redox state of Kelch13 determine the artemisinin susceptibility of Plasmodium falciparum

Redox Biology, 2021
Decreased susceptibilities of the human malaria parasite Plasmodium falciparum towards the endoperoxide antimalarial artemisinin are linked to mutations of residue C580 of PfKelch13, a homologue of the redox sensor Keap1 and other vertebrate BTB-Kelch ...
Robin Schumann, Eileen Bischoff, Severina Klaus, Sophie Möhring, Julia Flock, Sandro Keller, Kim Remans, Markus Ganter, Marcel Deponte   +8 more
doaj   +1 more source

A detailed protocol for expression, purification, and activity determination of recombinant SaCas9

STAR Protocols, 2022
Summary: Recombinant SaCas9 is useful for a broad range of applications in the context of genome editing, especially when the specific protospacer adjacent motifs of other Cas9 derivatives are missing.
Franziska Flottmann, Greta Marie Pohl, Jan Gummert, Hendrik Milting, Andreas Brodehl   +4 more
doaj   +1 more source

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

STAR Protocols, 2021
Summary: Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA.
Ruiliang Zhao, Yang Yang, Ke Yang, Wenyuan Han   +3 more
doaj   +1 more source

Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach

STAR Protocols, 2023
Summary: Here, we describe a protocol for artificially generating hetero-oligomeric protein complexes from the homo-oligomers using a sequential denaturation-renaturation strategy, followed by a modified affinity chromatography protocol used for their ...
Aasna L. Parui, Kakoli Bose
doaj   +1 more source

Efficient purification and assembly of ribonucleoprotein complex for interaction analysis by MST assay coupled with GaMD simulations

STAR Protocols, 2021
Summary: Here, we describe a generic protocol for monitoring protein-RNA interaction using a cleavable GFP fusion of a recombinant RNA-binding protein.
Yunrong Gao, Dongdong Cao, Shristi Pawnikar, Sana Akhter, Yinglong Miao, Bo Liang   +5 more
doaj   +1 more source