Results 11 to 20 of about 568,309 (314)
Express Your LOV: An Engineered Flavoprotein as a Reporter for Protein Expression and Purification [PDF]
PLoS ONE, 2012 In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function. Specifically, we used iLOV, an enhanced and more photostable variant of LOV. A pET-based plasmid for protein expression was constructed, encoding a C terminal iLOV-octahistidine
Gawthorne, J.A. +7 more
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Advances in the expression and purification of human PARP1: A user-friendly protocol [PDF]
, 2023 The PARP1 (Poly (ADP-ribose) polymerase 1) enzyme is essential for single and double-strand break repair in humans. Alterations affecting PARP1 activity have severe consequences for human health and are associated with pathologies like cancer, and ...
Bruno A. Salgueiro +9 more
core +1 more source
Expression and Purification of an Intrinsically Disordered Protein [PDF]
, 2020 Intrinsically disordered proteins (IDPs) describe a group of proteins that do not have a regular tertiary structure and typically have very little ordered secondary structure. Despite not following the biochemical dogma of "structure determines function" and "function determines structure," IDPs have been identified as having numerous biological ...
Karamjeet K, Singh, Steffen P, Graether
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Structure-based screening of binding affinities via small-angle X-ray scattering
IUCrJ, 2020 Protein–protein and protein–ligand interactions often involve conformational changes or structural rearrangements that can be quantified by solution small-angle X-ray scattering (SAXS). These scattering intensity measurements reveal structural details of
Po-chia Chen +3 more
doaj +1 more source
Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry
STAR Protocols, 2023 Summary: tRNA-bound amino acids often need to be identified, for instance, in cases where different amino acids compete for binding to the same tRNA.
Marc Finkler +5 more
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STAR Protocols, 2023 Summary: In membrane proteins, a transmembrane region and a juxtamembrane region play important roles in its function. Here, we present a protocol for characterizing membrane protein dynamics between the juxtamembrane region of the single transmembrane ...
Takeshi Sato, Akira Shinohara
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Functional in vitro and in vivo analysis of biosynthetic genes by heterologous expression in E. coli
STAR Protocols, 2023 Summary: Biosynthetic gene clusters of natural products often harbor genes of unknown function, which are difficult to characterize. Here, we present a protocol for the functional analysis in vitro and in vivo of these biosynthetic genes by heterologous ...
Jil-Christine Kramer +3 more
doaj +1 more source
Antibodies, 2014 Reduced culture temperature is an increasingly popular practice to improve recombinant protein yields in CHO cells. Recent studies have attributed the enhancement of protein titers at sub-physiological temperatures to increased mRNA levels as well as ...
Megan Mason +4 more
doaj +1 more source
STAR Protocols, 2023 Summary: Dynamic macromolecular complexes containing a large number of components are often difficult to study using conventional approaches, such as immunoblotting.
Petra Páleníková +2 more
doaj +1 more source
Protocol for reconstitution of oligomeric assembly of NAIP5-NLRC4 inflammasome in vitro
STAR Protocols, 2023 Summary: Inflammasomes are multimeric protein complexes that have crucial functions in innate immunity. Here, we present a protocol to reconstitute the PELO-driven assembly of NAIP5-NLRC4 inflammasome in vitro.
Xiurong Wu, Jiahuai Han
doaj +1 more source