Results 201 to 210 of about 292,859 (260)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Robust cis-by-trans epistasis in the human plasma proteome highlights an ABO-centered interaction network. [PDF]

Am J Hum Genet
Li J, Liu X.
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Structural basis of internal Vangl2 peptide recognition by the Dvl2 PDZ domain. [PDF]

Mol Biomed
Yang H, Deng H, Chen K, Zhang P, Xie N, Shen G.   +5 more
europepmc   +1 more source

Mapping the dynamic plant interactome: from in vitro assays to in vivo quantitative approaches. [PDF]

Plant Methods
Hussain MA, Hafeez AH, Noor I, Shakoor A, Hussain H, Gholizadeh F, Sohail H.   +6 more
europepmc   +1 more source

Peptidomics-guided 3D structural-mechanistic investigation of bovine colostrum peptides reveals multi-targeted <i>in silico</i> and <i>in vitro</i> antidiabetic and antioxidant potential. [PDF]

Food Chem X
Patil SM, Ramu R, Rajagopal K, Kameshwar VH.   +3 more
europepmc   +1 more source

Molecular Mechanisms Underlying Cucumber Resistance to Cucumber Green Mottle Mosaic Virus: From Host-Virus Interactions to Precision Breeding Strategies. [PDF]

Int J Mol Sci
Yu X, Zhu Y, Wang X, Sun Y, Gao L, Zhou S, Xin M, Zhang P.   +7 more
europepmc   +1 more source

HuRInterDB: A Database Exploring the Landscape of lncRNA-Protein Interactions. [PDF]

Comput Struct Biotechnol J
Xie Q, Sun W, Yan J.
europepmc   +1 more source