Results 21 to 30 of about 543,220 (340)

A Yeast BiFC-seq Method for Genome-wide Interactome Mapping

Genomics, Proteomics & Bioinformatics, 2022
Genome-wide physical protein–protein interaction (PPI) mapping remains a major challenge for current technologies. Here, we reported a high-efficiency BiFC-seq method, yeast-enhanced green fluorescent protein-based bimolecular fluorescence ...
Limin Shang, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Yanzhi Yuan, Chunyan Tian, Ming Ni, Xiaochen Bo, Li Zhang, Dong Li, Fuchu He, Jian Wang   +11 more
doaj   +1 more source

Identification of dysregulated pathways through SLC30A8 protein interaction in type 1 diabetes mellitus

AIMS Molecular Science, 2021
Objective: The aim of the current study was to explore the gene enrichment and dysregulated pathways on the basis of interaction network analysis of SLC30A8 in type 1 diabetes mellitus (T1DM).
Afreen Bhatty, Zile Rubab, Hafiz Syed Mohammad Osama Jafri, Sheh Zano   +3 more
doaj   +1 more source

ARL3 mutations cause Joubert syndrome by disrupting ciliary protein composition [PDF]

, 2018
Joubert syndrome (JBTS) is a genetically heterogeneous autosomal recessive neurodevelopmental ciliopathy. We investigated further the underlying genetic etiology of Joubert syndrome by studying two unrelated families in whom JBTS was not associated ...
Alhashem, Amal, Alkanderi, Sumaya, Alkuraya, Fowzan S., Cairns, George, Edwards, Noel, Elmaghloob, Yasmin, Ewida, Nour, Ismail, Shehab, Miles, Colin G., Molinari, Elisa, Ramsbottom, Simon A., Rice, Sarah J., Sammut, Veronica, Sayer, John A., Shaheen, Ranad, Srivastava, Shalabh, Steel, David H., Stephen, Louise A., White, Kathryn   +18 more
core   +1 more source

A Rice Kinase-Protein Interaction Map     [PDF]

Plant Physiology, 2008
AbstractPlants uniquely contain large numbers of protein kinases, and for the vast majority of the 1,429 kinases predicted in the rice (Oryza sativa) genome, little is known of their functions. Genetic approaches often fail to produce observable phenotypes; thus, new strategies are needed to delineate kinase function.
Ding, Xiaodong, Richter, Todd, Chen, Mei, Fujii, Hiroaki, Seo, Young Su, Xie, Mingtang, Zheng, Xianwu, Kanrar, Siddhartha, Stevenson, Rebecca A, Dardick, Christopher, Li, Ying, Jiang, Hao, Zhang, Yan, Yu, Fahong, Bartley, Laura E, Chern, Mawsheng, Bart, Rebecca, Chen, Xiuhua, Zhu, Lihuang, Farmerie, William G, Gribskov, Michael, Zhu, Jian-Kang, Fromm, Michael E, Ronald, Pamela C, Song, Wen-Yuan   +24 more
openaire   +3 more sources

Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states

eLife, 2016
To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away
Dan Tan, Qiang Li, Mei-Jun Zhang, Chao Liu, Chengying Ma, Pan Zhang, Yue-He Ding, Sheng-Bo Fan, Li Tao, Bing Yang, Xiangke Li, Shoucai Ma, Junjie Liu, Boya Feng, Xiaohui Liu, Hong-Wei Wang, Si-Min He, Ning Gao, Keqiong Ye, Meng-Qiu Dong, Xiaoguang Lei   +20 more
doaj   +1 more source

CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping. [PDF]

, 2017
Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase ...
Bartlett, Anna, Castanon, Rosa, Ecker, Joseph R, Feeney, Joseph, Galli, Mary, Garza, Renee M, Goubil, Adeline, Huang, Shao-Shan C, MacWilliams, Andrew, Nery, Joseph R, O'Malley, Ronan, Trigg, Shelly A, Zhang, Zhuzhu Z   +12 more
core   +2 more sources

Duchenne Muscular Dystrophy (DMD) Protein-Protein Interaction Mapping. [PDF]

Iran J Child Neurol, 2017
Duchenne muscular dystrophy (DMD) is one of the mortal diseases, subjected to study in terms of molecular investigation. In this study, the protein interaction map of this muscle-wasting condition was generated to gain a better knowledge of interactome profile of DMD.Applying Cytoscape and String Database, the protein-protein interaction network was ...
Rezaei Tavirani M, OkHOVATIAN F, Zamanian Azodi M, Rezaei Tavirani M.   +3 more
europepmc   +3 more sources

MAP-ping Unconventional Protein-DNA Interactions [PDF]

Cell, 2009
Control of gene expression depends on a myriad of protein-DNA interactions, and the number of proteins involved just got larger. In this issue, Hu et al. (2009) identify hundreds of human proteins that bind to DNA, including many surprises such as the protein kinase ERK2 (MAPK1) that now appears to control gene expression directly.
Dioum, Elhadji M., Wauson, Eric M., Cobb, Melanie H.   +2 more
openaire   +2 more sources

Herpes simplex virus ICP27 protein directly interacts with the nuclear pore complex through NUP62, inhibiting host nucleocytoplasmic transport pathways [PDF]

, 2012
The herpes simplex virus ICP27 protein is important for the expression and nuclear export of viral mRNAs. Although several binding sites have been mapped along the ICP27 sequence for various RNA and protein partners including the transport receptor TAP ...
Arnold, Aubert, Bachi, Bonifaci, Braun, Bryant, Chen, Chen, Copeland, Cullen, Darshan, Ellison, Ellison, Escudero-Paunetto, Faria, Fischer, Flach, Fontaine-Rodriguez, Fontoura, Fornerod, Fridell, Gerace, Gruffat, Grüter, Gustin, Gustin, Gustin, Han, Hardwicke, Hernandez, Hiriart, Hofemeister, Hutten, Johnson, Kambach, Koffa, Larralde, Lengyel, Lengyel, Li, Lilley, Long, Macaulay, Malik, Malik, McCarthy, Mears, Mears, Mears, Meyer, Michael, Nelson, Park, Pasdeloup, Pasquinelli, Pearson, Pellet, Petersen, Phelan, Piñol-Roma, Pollard, Pritchard, Rice, Rice, Rice, Rice, Rojas, Saavedra, Sandri-Goldin, Satterly, Schmitt, Sekulovich, Siomi, Smith, Soliman, Soliman, Souki, Strambio-De-Castillia, Strässer, Tsuji, Wagstaff, Wente, Zhi, Zhi   +83 more
core   +3 more sources

Protein-protein interaction map inference using interacting domain profile pairs [PDF]

Bioinformatics, 2001
Abstract A number of predictive methods have been designed to predict protein interaction from sequence or expression data. On the experimental front, however, high-throughput proteomics technologies are starting to yield large volumes of protein-protein interaction data.
J, Wojcik, V, Schächter
openaire   +2 more sources