Results 131 to 140 of about 56,071 (291)

Programmable Domestication: CRISPR, Pan‐Genomics and System Level Engineering for Next‐Generation Crops

Plant Biotechnology Journal, EarlyView.
Conceptual overview of programmable de novo domestication from genetic diversity discovery to crop deployment. The framework connects diverse germplasm and genomic resources with three trajectory pathways. Adaptive Rescue, Agronomic Refinement and Novel Chassis defined by biological distance, engineering complexity and breeding objectives.
Muhammad Mubashar Zafar, Hina Firdous, Ayesha Siddiqua, Ayesha Naveed, Abdul Razzaq, Sadam Munawar, Aqsa Ijaz, Zunaira Anwar, Sezai Ercisli, Xuefei Jiang, Qiao Fei   +10 more
wiley   +1 more source

Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.

BMC Plant Biology
Background Brassica oleracea L. is a key plant in the Brassicaceae family, known for popular vegetables like cabbage, broccoli, kale and collard. Collard (B. oleracea var.
Katarzyna Stelmach-Wityk, Kamil Szymonik, Ewa Grzebelus, Agnieszka Kiełkowska   +3 more
doaj   +1 more source

Studies on the protoplast of Phalaenopsisy hybrida HORT.

, 2014
使用蝴蝶蘭無菌播種的實生苗葉片以及嘉德麗亞蘭花瓣為材料，獲得較多原生質體的處理是以D-mannitol的濃度為0.7M，MES buffer 20mM，MgCl2 5mM，Ph5.6的基本液。添加酵素的配方，在蝴蝶蘭葉片為Cellulase "Onozuka" R-10 1.0%，Macerozyme R-10 0.5%，Pectolyase Y-23 0.1%。在嘉德麗蘭花瓣為Cellulase "Onozuka" R-10 2.0%，Macerozyme R-10 0.5%，Driselase 0 ...
黃敏展, Huang, Min-Chang
core  

Single‐Cell Transcriptomics Reveals the FA9–VAP Module Regulating Fatty Acid Accumulation in Soybean Seeds

Plant Biotechnology Journal, EarlyView.
ABSTRACT As a major commercial legume crop, soybean ranks among the world's most significant sources of edible oil and plant protein. We previously identified a SEIPIN homologue (FA9) at the fatty acid 9 locus that promotes fatty acid accumulation in soybean.
Hui Li, Tianshu Li, Xiaorui Xu, Jinhang Cui, Xin Chen, Jingyi Huang, Jia Song, Cui Mu, Xinyu Hong, Chunyan Liu, Ning Wang, Xue Han, Sui Wang, Qingshan Chen, Zhaoming Qi   +14 more
wiley   +1 more source

Plant cell protoplast isolation v1

, 2016
A protocol for isolation of protoplast from plant cells adapted from Yoo et al. 2007 (http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.htm). Procedure was optimized for use on Arabidopsis thaliana tissue.
Steven Burgess
core   +1 more source

HvPHO1 Controls a Glucose 1‐Phosphate–SnRK1 Signalling Module That Coordinates Starch Accumulation in Barley Endosperm

Plant Biotechnology Journal, EarlyView.
ABSTRACT Developing barley grains must balance the use of imported sucrose between soluble sugars and storage starch, but how this balance is controlled remains unclear. Here we show that loss of the plastidial α‐glucan phosphorylase HvPHO1 causes starch and soluble sugars to accumulate concurrently in barley grains.
Yulong Li, Yujia Chen, Jing Liu, Ping Yang, Guangqi Gao, Carlos Guzmán, Qiang Xu, Yazhou Zhang, Yanlin Liu, Pengfei Qi, Mei Deng, Shoufen Dai, Jian Ma, Guoyue Chen, Jirui Wang, Youliang Zheng, Yuming Wei, Qiantao Jiang   +17 more
wiley   +1 more source

The Transformation and Protein Expression of the Edible Mushroom Stropharia rugosoannulata Protoplasts by Agrobacterium-tumefaciens-Mediated Transformation

Journal of Fungi
Stropharia rugosoannulata is a cultivated edible mushroom characterized by its nutritional composition and efficient cellulolytic enzymatic systems.
Dongjie Yin, Hairong Xiong
doaj   +1 more source

Protoplast Fusion and Brassica Improvement

, 2003
76-84Brassica coenospecies has been bestowed with a large collection of wild and weedy relatives, which could act as donors of genes controlling agronomically important traits like disease resistance, yield attributes, modified fatty acid composition
Prakash, S, Bhat, S R, Chopra, V L, Kirti, P B   +3 more
core  

A modified method for transformation of Fusarium graminearum

Journal of Crop Protection, 2013
One of the important technical obstacles in the study of many filamentous fungi is the development of efficient transformation system. Transformation of filamentous fungi is difficult because they have a cell wall and for most frequently used approaches ...
Sohrab Moradi, Forough Sanjarian, Naser Safaie, Amir Mousavi, Gholam Reza Bakhshi Khaniki   +4 more
doaj  

Harnessing Natural Diversity and Rational Design for Enhanced Plant Immunity

Plant, Cell &Environment, EarlyView.
ABSTRACT Plant pathogens cause crop loss. Genetic resistance, through natural genetic diversity or engineered resistance, can be highly effective in protecting plants from infection. Genetic resistance from wild relatives of crops has been successfully introduced into crops to control pathogen infection.
Nathan Diplock, Jennifer D. Lewis
wiley   +1 more source