Results 121 to 130 of about 56,071 (291)

An efficient cucumber (Cucumis sativus L.) protoplast isolation and transient expression system

, 2013
The transient gene expression system using plant protoplasts has become widely used for high-throughput analysis and functional characterization of genes.
Hong-Yu Huang, Zhenyu Wang, Jin-Tao Cheng, Wen-Chao Zhao, Xin Li, Hongyun Wang, Zhen-Xian Zhang, X. Sui   +7 more
semanticscholar   +1 more source

Coordination of drought‐adaptive root growth plasticity through auxin homeostasis by the ZmSPL12–ZmYUC5 module in maize

New Phytologist, EarlyView.
Proposed model of ZmSPL12‐mediated drought resistance through auxin regulation. Summary Climate change‐induced drought increasingly threatens global maize production. Drought‐adaptive root plasticity in maize depends on auxin signaling, yet how auxin homeostasis is dynamically tuned in response to water deficit remains unclear.
Hua Xu, Lei Ma, Qingyun Zhang, Zhigang Zheng, Jiajing Li, Sen Yang, Yanfeng Zhao, Lian Jin, Binbin Zhao, Zhijing Bai, Jiyuan Cao, Youpeng Tao, Junjie Zou, Baobao Wang, Shujing Cheng, Jinfang Chu, Jiafan Liu, Haiyang Wang, Miaoyun Xu   +18 more
wiley   +1 more source

Changes of DNA methylation and hydroxymethylation in plant protoplast cultures [PDF]

, 2013
Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms.
Ondřej, Vladan, Moricová, Pavla, Navrátilová, Božena, Luhová, Lenka   +3 more
core  

High‐Contiguity Haplotype‐Resolved Genome Assembly of the Hexaploid Actinidia valvata Rootstock Sheds Light on Waterlogging Resistance Gene

Plant Biotechnology Journal, EarlyView.
ABSTRACT Polyploidisation, creating redundant or diverged copies of the genome, is a major driving force in plant evolution, diversification and environmental adaptation, including for the kiwifruit genus (Actinidia Lindl.). We present a high‐contiguity, haplotype‐resolved genome assembly of the hexaploid Actinidia valvata rootstock cultivar ...
Miaomiao Lin, Zhi Li, Cecilia H. Deng, Congcong Li, Zhengzhen Zhang, Yukuo Li, Qina Zhang, Peng Zhang, Leiming Sun, Mingyu Liu, Jinbao Fang, Xiujuan Qi   +11 more
wiley   +1 more source

Single‐Nucleus Transcriptome Analysis Provides New Insights Into B Chromosome Elimination in Sorghum

Plant Biotechnology Journal, EarlyView.
ABSTRACT B chromosomes are supernumerary entities found in many plant species, with some exhibiting tissue‐specific elimination. In Sorghum purpureosericeum, extensive B chromosome elimination occurs during embryogenesis affecting most of the embryonic organs, leaving the B chromosome maintained mainly in limited regions of meristems.
T. Bojdová, P. Abaffy, L. Valihrach, M. Karafiátová, J. Bartoš   +4 more
wiley   +1 more source

Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium

, 2007
Vital protoplasts from Spathiphyllum wallisii ‘Alain’ and Anthurium scherzerianum ‘238’ were isolated from both somatic embryos and leaves. The highest yields were obtained when 1.5% cellulase, 0.5% macerase and 0.5% driselase were used for Spathiphyllum
Werbrouck, Stefaan, Van Huylenbroeck, Johan, Duquenne, Barbara, Eeckhaut, Tom   +3 more
core   +1 more source

The OsWRKY15‐OsSUT3 Regulatory Module Enhances Rice Pollen Fertility by Mediating Sucrose Allocation to Anthers

Plant Biotechnology Journal, EarlyView.
ABSTRACT Sucrose transport and starch accumulation are crucial carbohydrate metabolic processes in rice, playing essential roles during late pollen development to ensure pollen maturation, fertility and high grain production. However, the molecular mechanisms regulating sucrose transport and starch accumulation during rice pollen development remain ...
Qiuping Li, Chunlong Zhang, Shuaibing Wang, Jin Chen, Yandong Sun, Wenqing Ma, Yu Yong, Qinghui Yang, Lijuan Chen, Jiancheng Wen, Dandan Li   +10 more
wiley   +1 more source

Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato

Frontiers in Genome Editing
Homology-directed repair (HDR) holds great promise for plant genetic engineering but remains challenging due to its inherently low efficiency in gene editing applications.
Matías N. González, Neha Salaria, Martin Friberg, Ying Liu, Josefin Alverup, Mariette Andersson, Per Hofvander   +6 more
doaj   +1 more source

A MYB‐Related Transcriptional Module Cooperatively Represses Xylem Development in Poplar

Plant Biotechnology Journal, EarlyView.
ABSTRACT MYB transcription factors play crucial roles in regulating plant growth and development, but the functions of the MYB‐related subfamily in woody plants remain poorly understood. In this study, we characterized PagMYBR028, an MYB‐related transcription factor from poplar ‘84 K’ (Populus alba × Populus glandulosa), which was predominantly ...
Gaofeng Fan, Jiahui Jiang, Zihan Cheng, Feixiang Ma, Zhanling Sun, Zelin Li, Yu Qi, Guanzheng Qu, Tingbo Jiang   +8 more
wiley   +1 more source

Multiplexed Engineering of Disease Resistance and Lodging Tolerance in Wheat Via a Single Xylan Modifying Glycosyltransferase

Plant Biotechnology Journal, EarlyView.
ABSTRACT Breeding crops with robust disease resistance often compromises yield, a persistent challenge in agricultural improvement. We report that modifying cell wall architecture through a xylan‐specific glycosyltransferase successfully breaks this trade‐off in wheat.
Shufang Sun, Chunhui Li, Zehua Wang, Chunyang Ma, Xinhui Niu, Qunqing Wang, Qian Xu   +6 more
wiley   +1 more source