Results 101 to 110 of about 26,488 (218)

An Efficient System for Mesophyll Protoplast Isolation, Purification, and Transformation in Loquat: Studies on Fluorescent Marker Analysis and Subcellular Localization

Horticulturae
Loquat (Eriobotrya japonica Lindl.) is one of the most important subtropical evergreen fruit trees. However, due to the lack of widely applicable genetic transformation platforms, the research about gene functional characterization and molecular ...
Shuming Wang, Liyun Wang, Zhixiang Liu, Yan Xia, Danlong Jing, Qigao Guo, Guolu Liang, Qiao He   +7 more
doaj   +1 more source

Single‐Nucleus Transcriptome Analysis Provides New Insights Into B Chromosome Elimination in Sorghum

Plant Biotechnology Journal, EarlyView.
ABSTRACT B chromosomes are supernumerary entities found in many plant species, with some exhibiting tissue‐specific elimination. In Sorghum purpureosericeum, extensive B chromosome elimination occurs during embryogenesis affecting most of the embryonic organs, leaving the B chromosome maintained mainly in limited regions of meristems.
T. Bojdová, P. Abaffy, L. Valihrach, M. Karafiátová, J. Bartoš   +4 more
wiley   +1 more source

Protoplast Fusion and Brassica Improvement

, 2003
76-84Brassica coenospecies has been bestowed with a large collection of wild and weedy relatives, which could act as donors of genes controlling agronomically important traits like disease resistance, yield attributes, modified fatty acid composition
Prakash, S, Bhat, S R, Chopra, V L, Kirti, P B   +3 more
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The OsWRKY15‐OsSUT3 Regulatory Module Enhances Rice Pollen Fertility by Mediating Sucrose Allocation to Anthers

Plant Biotechnology Journal, EarlyView.
ABSTRACT Sucrose transport and starch accumulation are crucial carbohydrate metabolic processes in rice, playing essential roles during late pollen development to ensure pollen maturation, fertility and high grain production. However, the molecular mechanisms regulating sucrose transport and starch accumulation during rice pollen development remain ...
Qiuping Li, Chunlong Zhang, Shuaibing Wang, Jin Chen, Yandong Sun, Wenqing Ma, Yu Yong, Qinghui Yang, Lijuan Chen, Jiancheng Wen, Dandan Li   +10 more
wiley   +1 more source

Protoplast Isolation and Regeneration from Gracilaria changii (Gracilariales, Rhodophyta)

, 2008
The tropical agarophyte Gracilaria changii has been much researched and documented by the Algae Research Laboratory, University of Malaya, especially with regards to its potential as a seaweed bioreactor for valuable compounds. Protoplast regeneration of
Yeong, Hui Yin, Khalid, Norzulaani, Phang, Siew Moi   +2 more
core   +1 more source

A MYB‐Related Transcriptional Module Cooperatively Represses Xylem Development in Poplar

Plant Biotechnology Journal, EarlyView.
ABSTRACT MYB transcription factors play crucial roles in regulating plant growth and development, but the functions of the MYB‐related subfamily in woody plants remain poorly understood. In this study, we characterized PagMYBR028, an MYB‐related transcription factor from poplar ‘84 K’ (Populus alba × Populus glandulosa), which was predominantly ...
Gaofeng Fan, Jiahui Jiang, Zihan Cheng, Feixiang Ma, Zhanling Sun, Zelin Li, Yu Qi, Guanzheng Qu, Tingbo Jiang   +8 more
wiley   +1 more source

Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato

Frontiers in Genome Editing
Homology-directed repair (HDR) holds great promise for plant genetic engineering but remains challenging due to its inherently low efficiency in gene editing applications.
Matías N. González, Neha Salaria, Martin Friberg, Ying Liu, Josefin Alverup, Mariette Andersson, Per Hofvander   +6 more
doaj   +1 more source

Multiplexed Engineering of Disease Resistance and Lodging Tolerance in Wheat Via a Single Xylan Modifying Glycosyltransferase

Plant Biotechnology Journal, EarlyView.
ABSTRACT Breeding crops with robust disease resistance often compromises yield, a persistent challenge in agricultural improvement. We report that modifying cell wall architecture through a xylan‐specific glycosyltransferase successfully breaks this trade‐off in wheat.
Shufang Sun, Chunhui Li, Zehua Wang, Chunyang Ma, Xinhui Niu, Qunqing Wang, Qian Xu   +6 more
wiley   +1 more source

Programmable Domestication: CRISPR, Pan‐Genomics and System Level Engineering for Next‐Generation Crops

Plant Biotechnology Journal, EarlyView.
Conceptual overview of programmable de novo domestication from genetic diversity discovery to crop deployment. The framework connects diverse germplasm and genomic resources with three trajectory pathways. Adaptive Rescue, Agronomic Refinement and Novel Chassis defined by biological distance, engineering complexity and breeding objectives.
Muhammad Mubashar Zafar, Hina Firdous, Ayesha Siddiqua, Ayesha Naveed, Abdul Razzaq, Sadam Munawar, Aqsa Ijaz, Zunaira Anwar, Sezai Ercisli, Xuefei Jiang, Qiao Fei   +10 more
wiley   +1 more source

Molecular Characterization of Intraspecific Protoplast Fusion in Trichoderma Harzianum [PDF]

, 2011
: Protoplast was isolated from two fungicide tolerant mutants PTz-C and PTz-F of T. harzianum, obtained from 18 h old mycelium digested by Novozyme 234 and fused by polyethylene glycol (PEG). The frequency of fusion resulting in double fungicide tolerant
Ragaa A Eissa, M M, H A Hamza, Hassan
core