Results 91 to 100 of about 56,071 (291)

Hevea brasiliensis seeds: A novel sustainable feedstock for ethanol production

open access: yesVietnam Journal of Chemistry, EarlyView.
Abstract The rubber tree (Hevea brasiliensis) is cultivated on a large scale mainly for the extraction of latex in Brazil and Southeast Asia, also generating large quantities of seeds. These seeds currently do not have large‐scale applications and may thus be considered as a residue.
Giovano Tochetto   +4 more
wiley   +1 more source

Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Glycine max [L.] Merr.)

open access: yesFrontiers in Plant Science
Soybean is important crop species in agriculture, food science, and biotechnology due to their valuable components. The exploration of soybean genetic traits is being highlighted for the advancement of research in various aspects.
Kihwan Kim   +7 more
doaj   +1 more source

Optimization Conditions of Wheat Mesophyll Protoplast Isolation

open access: yes, 2016
The research object of this study is “ML7113” wheat leaf, which is used to isolate protoplast with enzyme hydrolysis method. Three main effectors—the concentration of mannitol, enzymolysis time and centrifugal force, affect the production and vitality of
X. Jia   +3 more
semanticscholar   +1 more source

Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.)

open access: yesPlant Cell Reports, 2015
Key messageA switchgrass protoplast system was developed, achieving a cost reduction of ~1000-fold, a threefold increase in transformation efficiency, and a fourfold reduction in required DNA quantity compared to previous methods.AbstractIn recent years,
K. P. Burris   +4 more
semanticscholar   +1 more source

PEG-Mediated Protoplast Transformation of Penicillium sclerotiorum (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

open access: yesJournal of Fungi
Protoplast-based transformation is a vital tool for genetic studies in fungi, yet no protoplast method existed for P. sclerotiorum-scaumcx01 before this study. Here, we optimized protoplast isolation, regeneration, and transformation efficiency.
Israt Jahan   +5 more
doaj   +1 more source

Role of the tomato MARS1/ROUGH gene encoding a LYSINE‐SPECIFIC HISTONE DEMETHYLASE 1 in adventitious root and fruit skin formation

open access: yesJournal of Integrative Plant Biology, EarlyView.
The tomato mars1/rough mutant displays enhanced root regeneration and rough fruits due to ectopic cell proliferation. The causal gene encodes a lysine‐specific histone demethylase that normally maintains gene silencing. Its loss alters histone methylation, upregulating several genes, including those B‐type cyclins involved in tissue‐specific cellular ...
Eduardo Larriba   +14 more
wiley   +1 more source

A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L

open access: yesBMC Plant Biology
Background Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal and industrial potential of Cannabis sativa L., developing an efficient protoplast-to-plant regeneration protocol is ...
Katarzyna Stelmach-Wityk   +3 more
doaj   +1 more source

Early protoplast culture and partial regeneration in Cannabis sativa: gene expression dynamics of proliferation and stress response

open access: yesFrontiers in Plant Science
Cannabis sativa L. is a plant of significant interest due to its high content of biologically active compounds, durable fibers, and bioeconomic potential.
Daniel Král   +2 more
doaj   +1 more source

Implication of mating type and cytoplasm factors in asymmetric distribution of two types of monokaryons recovered from dikaryons in Lentinula edodes

open access: yes浙江大学学报. 农业与生命科学版, 2008
The genetics background for the phenomenon of significantly distortion to the expected 1∶1 ratio of the two constitute nucleus in Lentinula edodes was investigated after protoplast monokaryotization.
CHENG Shui-ming, LIN Fan-xue, XU Chun
doaj   +1 more source

Protoplast stability after encapsulation.

open access: yes, 2018
(A) Bright field and (B) chlorophyll fluorescence micrographs of individual M. polymorpha protoplast encapsulated in microdroplets.
Jim Haseloff (91083)   +6 more
core   +1 more source

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