Results 71 to 80 of about 56,071 (291)

Solutions used for protoplast isolation and transfection.

, 2023
Solutions used for protoplast isolation and transfection.
Michael J. Thomson (8477751), Endang M. Septiningsih (6003947), Aya Bridgeland (15188045), Sudip Biswas (8506077), Nikolaos Tsakirpaloglou (15188048)   +4 more
core   +1 more source

Pectinase‐Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development

Biotechnology and Bioengineering, EarlyView.
This picture demonstrates a unified strategy for sustainable pectinase production, focusing on microbial bioproduction, innovative strategies in enzyme engineering (Protein engineering, rational enzyme design, directed evolution and heterologous expression) and various applications in industry, including food, textiles, pulp and paper, agriculture and ...
Nisha Sharma, Dimple Tanwar, Usha Grewal, Murugesan Mohana Keerthi, Yogesh K. Ahlawat, Nitin Pandey, Chirag G. Makvana   +6 more
wiley   +1 more source

Optimization of Protoplast Preparation Conditions in Lyophyllum decastes and Transcriptomic Analysis Throughout the Process

Journal of Fungi
Protoplasts are essential tools for genetic manipulation and functional genomics research in fungi. This study systematically optimized protoplast preparation conditions and examined transcriptional changes throughout the preparation and regeneration ...
Xiaobin Li, Ying Qin, Yufei Kong, Samantha Chandranath Karunarathna, Yunjiang Liang, Jize Xu   +5 more
doaj   +1 more source

Induction and Enhancement of Bacteriocin Production (Review)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

BMC Biotechnology, 2016
BackgroundPhaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid transformation system is more imperative than ever.
Kalpana Nanjareddy, Manoj-Kumar Arthikala, L. Blanco, Elizabeth S. Arellano, M. Lara   +4 more
semanticscholar   +1 more source

Viscosity of concentrated mRNA solutions: Implications for downstream processing

Biotechnology Progress, EarlyView.
Abstract Messenger RNA (mRNA) therapeutics can be used to protect against infectious disease and for the treatment of cancers and genetic disorders. In order to obtain the desired therapeutic dosage, it is necessary to concentrate the mRNA to levels where the viscosity of the mRNA solutions can become significant.
Amin Javidanbardan, Fatemeh Najafi, Ziqiao Wang, Andrew L. Zydney   +3 more
wiley   +1 more source

Peroxisomes contribute to reactive oxygen species homeostasis and cell division induction in Arabidopsis protoplasts

Frontiers in Plant Science, 2015
The ability to induce Arabidopsis protoplasts to de-differentiate and divide provides a convenient system to analyse organelle dynamics in plant cells acquiring totipotency.
Terence Wei Yang Tiew, Michael B. Sheahan, Ray J. Rose   +2 more
doaj   +1 more source

Independent leaf and root responses to autumn freezing in perennial herbs: Insights from cutting and transplant manipulations

Grassland Research, EarlyView.
In this study, we compared two sampling approaches to assess autumn freezing resistance. Our goal was to determine whether the commonly employed cutting method, which disrupts root‐tissue connectivity, provides a valid measure of true freezing resistance.
Zhengjie Yan, Cheng Chen, Xin Jing, Shuai Ren, Junpeng Rui, Tao Wang, Jin‐Sheng He   +6 more
wiley   +1 more source

Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai

BMC Plant Biology
Background Angelica Gigas (Purple parsnip) is an important medicinal plant that is cultivated and utilized in Korea, Japan, and China. It contains bioactive substances especially coumarins with anti-inflammatory, anti-platelet aggregation, anti-cancer ...
Han-Sol Lee, Jong-Eun Han, Eun-Kyung Bae, Eun Yee Jie, Suk Weon Kim, Hyuk Joon Kwon, Hak Sung Lee, Soo-Ho Yeon, Hosakatte Niranjana Murthy, So-Young Park   +9 more
doaj   +1 more source

PROTOPLAST-BASED TECHNOLOGY FOR IMPLEMENTATION OF NEW BREEDING TECHNIQUES IN CHICORY spp. [PDF]

openChicory (Cichorium intybus L.) is a nutritionally and medicinally valuable plant with increasing significance in modern agriculture and biotechnology. This study aimed to optimize in vitro culture, protoplast isolation, and transfection protocols for
MUSARRAT, HADEENA
core