Results 71 to 80 of about 26,488 (218)

Plant cell protoplast isolation v1

, 2016
A protocol for isolation of protoplast from plant cells adapted from Yoo et al. 2007 (http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.htm). Procedure was optimized for use on Arabidopsis thaliana tissue.
Steven Burgess
core   +1 more source

Protoplast Isolation and Shoot Regeneration from Protoplast-Derived Callus of Petunia hybrida Cv. Mirage Rose

, 2020
Despite the increasing use of protoplasts in plant biotechnology research, shoot regeneration from protoplasts remains challenging. In this study, we investigated the factors involved in protoplast isolation, callus induction, and shoot regeneration in ...
Hyun Hee Kang, Aung Htay Naing, Chang Kil Kim   +2 more
core   +1 more source

Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Glycine max [L.] Merr.)

Frontiers in Plant Science
Soybean is important crop species in agriculture, food science, and biotechnology due to their valuable components. The exploration of soybean genetic traits is being highlighted for the advancement of research in various aspects.
Kihwan Kim, Kihwan Kim, Junseop Shin, Jeong-Dong Lee, Jeong-Dong Lee, Jeong-Dong Lee, Won-Chan Kim, Won-Chan Kim   +7 more
doaj   +1 more source

CP32, a novel anti‐oomycete cyclic peptide, interferes with cell‐wall deposition in Phytophthora infestans

Journal of Integrative Plant Biology, EarlyView.
The small cyclic peptide CP32 effectively inhibits Phytophthora infestans, the causal agent of potato and tomato late blight. Combined biochemical and phenotypic analyses demonstrate that CP32 disrupts cell wall biosynthesis, a key process in pathogen infection, resulting in a marked reduction of disease symptoms in plants.
Demetrio Marcianò, Stefano Rosa, Elena Marone Fassolo, Chiara Bertaso, Andrea Tagliani, Nina Gauri Capra, Federico Ballabio, Chiara Mizzotti, Giulia Rizzi, Sadia Fida Ullah, Lisa Kappel, Lucia Feni, Luca Tadini, Louise Jane Gourlay, Rosaria Russo, Vincent Bulone, Sabrina Pricl, Sandrine Ongeri, Sara Pellegrino, Carlo Camilloni, Silvia Laura Toffolatti, Vaibhav Srivastava, Paolo Pesaresi, Simona Masiero   +23 more
wiley   +1 more source

PEG-Mediated Protoplast Transformation of Penicillium sclerotiorum (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

Journal of Fungi
Protoplast-based transformation is a vital tool for genetic studies in fungi, yet no protoplast method existed for P. sclerotiorum-scaumcx01 before this study. Here, we optimized protoplast isolation, regeneration, and transformation efficiency.
Israt Jahan, Qilin Yang, Zijun Guan, Yihan Wang, Ping Li, Jian Yan   +5 more
doaj   +1 more source

Implication of mating type and cytoplasm factors in asymmetric distribution of two types of monokaryons recovered from dikaryons in Lentinula edodes

浙江大学学报. 农业与生命科学版, 2008
The genetics background for the phenomenon of significantly distortion to the expected 1∶1 ratio of the two constitute nucleus in Lentinula edodes was investigated after protoplast monokaryotization.
CHENG Shui-ming, LIN Fan-xue, XU Chun
doaj   +1 more source

Natural variation in the GNH1 promoter enhances plant height and grain yield in rice

Journal of Integrative Plant Biology, EarlyView.
Natural variation in the GRAIN NUMBER AND PLANT HEIGHT 1 (GNH1) promoter alleviates repression by the C2H2‐type transcription factor ZFP36, upregulating GNH1 expression and promoting auxin accumulation, thereby increasing rice plant height and grain yield. This elite allele serves as a valuable genetic resource for molecular breeding of high‐yield rice.
Hongwei Xing, Huan Wang, Sheng Wu, Yuanjie Li, Kun Zhang, Chuanqing Sun, Hongying Sun   +6 more
wiley   +1 more source

Temperature-sensitive, protoplast-forming os-1 varient of Neurospora- a few tricks. [PDF]

, 1982
Temperature-sensitive, protoplast-forming os-1 varient of Neurospora- a few ...
Selitrennikoff, C. P., C. P. Selitrennikoff   +1 more
core   +1 more source

Effects of macerozyme and cellulase enzymes combination ratio on yield and viability of cassava protoplast.

, 2022
Effects of macerozyme and cellulase enzymes combination ratio on yield and viability of cassava protoplast.
Asunta Mukami (14011413), Mathew Ngugi (10494139), Richard Oduor (7408637), Bicko Steve Juma (14011410), Wilton Mwema Mbinda (14204647), Cecilia Mweu (10494133)   +5 more
core   +1 more source

A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L

BMC Plant Biology
Background Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal and industrial potential of Cannabis sativa L., developing an efficient protoplast-to-plant regeneration protocol is ...
Katarzyna Stelmach-Wityk, Kamil Szymonik, Andrew Maxwell Phineas Jones, Ewa Grzebelus   +3 more
doaj   +1 more source