Results 61 to 70 of about 26,488 (218)
Liquid Phase Backscattered Scanning Electron Microscopy of Bacillus subtilis Spores
Small Methods, EarlyView.Graphene encapsulation enables liquid‐phase BSE‐SEM to visualize subsurface architecture of whole, hydrated Bacillus subtilis spores without staining. This approach is applied to visualize dormant spores and subsequent structural changes during spore germination, while imaging across a range of beam energies and Monte Carlo simulations provide a ...
Jack Bromley +8 more
wiley +1 more source
PROTOPLAST-BASED TECHNOLOGY FOR IMPLEMENTATION OF NEW BREEDING TECHNIQUES IN CHICORY spp. [PDF]
openChicory (Cichorium intybus L.) is a nutritionally and medicinally valuable plant with increasing significance in modern agriculture and biotechnology. This study aimed to optimize in vitro culture, protoplast isolation, and transfection protocols for
MUSARRAT, HADEENA
core
Protoplast Isolation - Incubation Buffer v1
, 2016 Instructions to make 50 mL of incubation buffer for protoplast isolation. Adapted from Yoo et al. 2007http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.html
Steven Burgess
core +1 more source
Plant regeneration via protoplast electrofusion in cassava
, 2020 Protoplast electrofusion between callus protoplasts of cultivar TMS60444 and mesophyll protoplasts of cultivar SC8 was performed as an approach for the genetic improvement of cassava.
Feng WEN +6 more
core +1 more source
Yeast, EarlyView.Yeast transformations are mechanistically poorly understood and could be made orders of magnitude more efficient by three approaches. (1) Investigate mechanisms of DNA uptake including DNA phase separation. (2) Account for growth state. (3) Use systematic statistical design of experiments to test combinations of factors that enhance transformation ...
Alistair Elfick, Edward W. J. Wallace
wiley +1 more source
A Rapid Lithium Ion‐Based Transformation Method for Kluyveromyces lactis
Yeast, EarlyView.ABSTRACT Kluyveromyces lactis is a budding yeast host useful for recombinant protein production; however, its transformation often relies on electroporation due to the perceived low efficiency of chemical methods, thereby limiting throughput. In this study, we developed a robust, small‐scale chemical transformation protocol (using 15 μL of competent ...
Daisuke Tsugama, Ziyi Zuo
wiley +1 more source
Journal of Integrative Plant Biology, EarlyView.The redox‐sensitive transcriptional repressor ZmMS1 coordinates reactive oxygen species homeostasis and lipid allocation for pollen exine and anther cuticle formation. Loss or precocious expression of ZmMS1 causes male sterility. Constitutive overexpression induces dwarfism and semi‐sterility. These findings enable flexible maize male‐sterility systems
Quancan Hou +13 more
wiley +1 more source
Formation of Bacterionema matruchotii Protoplast
, 1979 Protoplast formation with Bacterionema matruchotii was studied turbidimetrically and microscopically. The protoplasts were inducible during culture by addition of glycine, lysozyme and Ficoll to a medium containing Tween-80 and polyethylene glycol.
Toshiko Itoyama +2 more
core +1 more source
Agricultural and Food Science, 1991 Protoplast cultures were prepared from 6-day-old hypocotyls of six spring, seven winter cultivars of Brassica napus L. and one line of Brassica campestris L.
János Pauk +3 more
doaj
Journal of Integrative Plant Biology, EarlyView.Allelic variation in the promoter of the pyridoxal phosphate synthase OsPDX1.2 caused variation in the ACE‐box cis‐elements, resulting in differing OsPDX1.2 expression due to differences in binding affinity between the OsbZIP18 transcriptipn factor and the ACE‐box, contributing to variation in vitamin B6 levels and cold tolerance among rice varieties ...
Bi Wang +18 more
wiley +1 more source