Results 181 to 190 of about 56,071 (291)

Establishment of Protoplast Preparation and Genetic Transformation Methods in Two <i>Ilyonectria</i> Species. [PDF]

J Fungi (Basel)
Wang Y, Zhang W, Wang X, Zhang X, Lu X, Wang X, Gao W.   +6 more
europepmc   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3196-3207, September 2026.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Characterization of oxidative status in maize protoplasts under temperature and saline-alkali stresses. [PDF]

BMC Plant Biol
Wang W, Li J, Hu X, Liu Z, Cai D, Li X, Nie T, Li B, Chen X, Zhai Y, Chen G.   +10 more
europepmc   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3316-3328, September 2026.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Whole-Genome Analysis of Bacillus Licheniformis Ali5 and Synthesis of Lichenysin via Genome Shuffling. [PDF]

J Basic Microbiol
Pang Z, Zhang B, Tao Z, Wang F.
europepmc   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3231-3242, September 2026.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

Breeding of Early Maturity, High-Quality <i>Auricularia heimuer</i> Strains Using Protoplast Fusion. [PDF]

J Fungi (Basel)
Yue X, Song S, Zhang X, Zhao Y, Jiang Y, Zhou S, Zhang X, Han C, Dai X.   +8 more
europepmc   +1 more source

SIB1‐SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3458-3472, September 2026.
SEC23A mediates membrane trafficking between the endoplasmic reticulum (ER) and Golgi apparatus and interacts with the immunity protein SIGMA FACTOR‐BINDING PROTEIN1 in Arabidopsis. Under stress, both proteins relocate from the ER to chloroplasts, suppressing photosynthesis genes and enhancing defense genes.
Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia   +7 more
wiley   +1 more source

Aggregates-on-chip: a microfluidic device for crowding plant cells in culture. [PDF]

Front Plant Sci
Hessin AF, Orozaliev A, Mohammed MA, Van AD, Guillotin B, Song YA, Birnbaum KD.   +6 more
europepmc   +1 more source

How do secretory products cross the plant cell wall to be released? A new hypothesis involving cyclic mechanical actions of the protoplast.

Annals of Botany, 2016
É. A. Paiva
semanticscholar   +1 more source