Optimized Cas‐SF01 gene‐editing toolbox shortens flowering timing in commercial maize inbred JING724
The gene‐editing tool Cas‐SF01 was optimized to maximize its efficiency in maize. The Cas‐SF01‐TREX2 configuration was superior in enabling high‐purity gene mutations. This toolkit enabled commercial maize to flower seven days earlier without yield loss, thereby securing harvests and accelerating crop breeding.
Mengyuan Liu +11 more
wiley +1 more source
A Simplified Wheat Protoplast Transformation System and Guideline for Avoiding Protein Localization Artifacts. [PDF]
Li L +7 more
europepmc +1 more source
The phosphorylation status of the WRKY transcription factor WRKY10/MINI3, controls the timing of endosperm cellularization in Arabidopsis, thus regulating final seed size, offering a molecular understanding that could inform future agricultural breeding strategies. ABSTRACT Seed size is a critical factor in determining crop yield.
Min Ni +8 more
wiley +1 more source
Multifunctional Vitamin B<sub>1</sub>-Derived Fluorescent Copper Nanoclusters for Efficient Maize Protoplast Transformation. [PDF]
László N, Szabó M, Ferenc G, Ungor D.
europepmc +1 more source
Engineering glyphosate‐resistant watermelon by prime editing
Editing the watermelon EPSPS gene using a prime editing platform with visible markers created a non‐transgenic glyphosate‐resistant line. These robust heterozygous mutants tolerate field‐level herbicide doses without growth penalties, serving as ideal parental lines for crop breeding.
Shouwei Tian +7 more
wiley +1 more source
Protoplasts Isolation and Transient Transformation System Optimization for Poplar 84K (<i>Populus alba</i> × <i>Populus glandulosa</i>). [PDF]
Yu C, Yu H, Rui Y, Wang M.
europepmc +1 more source
A novel Cas12b cytosine base editor enables efficient C‐to‐T base editing in the rice genome
The base editor dAaCas12b accurately induces C‐to‐T mutations in rice. It recognizes unique TTTV DNA motifs with a wider editing window, and its optimized guide RNA boosts efficiency, expanding crop gene‐editing resources.
Yulei Wei +11 more
wiley +1 more source
PsORP1 anchors ER–Golgi MCSs to mediate PS/PI4P counter‐transport, and oxathiapiprolin targets the ORD to inhibit this lipid exchange. ABSTRACT Oxysterol‐binding protein (OSBP)‐related proteins (ORPs) are indispensable eukaryotic lipid transporters and promising targets for pharmaceutical and agrochemical exploitation.
Xiaofei Liu +6 more
wiley +1 more source
Engineering a cell wall-deficient protoplast-incorporated hydrogel for infected wound healing. [PDF]
Zeng Y +11 more
europepmc +1 more source
Methods to Study Mitochondrial Metabolism and Homeostasis in Fission Yeast
Yeast, Volume 43, Issue 5, Page 214-222, September 2026.
Ferran Gómez‐Armengol +2 more
wiley +1 more source

