Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system. [PDF]
Commun BiolZahn V +4 more
europepmc +1 more source
Optimized Protoplasts Preparation of Fungus <i>Magnaporthe oryzae</i> by Novel Myxolase F Lyase. [PDF]
J Fungi (Basel)Liu J +5 more
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Whole-Genome Analysis of Bacillus Licheniformis Ali5 and Synthesis of Lichenysin via Genome Shuffling. [PDF]
J Basic MicrobiolPang Z, Zhang B, Tao Z, Wang F.
europepmc +1 more source
A streamlined protocol for small-scale protoplast generation and CRISPR/Cpf1-mediated genome editing in <i>Fusarium oxysporum</i>. [PDF]
iScienceZheng JZ +4 more
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An efficient method for tissue-specific protoplast isolation suitable for single-cell RNA sequencing and transient gene expression analysis in saffron (Crocus sativus L.). [PDF]
BMC BiotechnolXi X +5 more
europepmc +1 more source
Genetic tools for transformation of Xyleborus ambrosia beetle fungal symbionts. [PDF]
Fungal Biol BiotechnolJoseph RA, Masoudi A, Keyhani NO.
europepmc +1 more source
Tiny but powerful: protoplast isolation in <i>Solanum melongena</i> L. and RNPs mediated genome editing. [PDF]
Front Plant SciFerrero M +5 more
europepmc +1 more source
Protoplasting and Transformation Using CRISPR/Cas9 in Coccidioides. [PDF]
Curr ProtocJackson KM +7 more
europepmc +1 more source
Process optimization for protoplast preparation from Grifola frondosa and development of a high-yield mutant with enhanced mycelial growth and protein production. [PDF]
BMC BiotechnolWang Q +7 more
europepmc +1 more source