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A streamlined protocol for small-scale protoplast generation and CRISPR/Cpf1-mediated genome editing in <i>Fusarium oxysporum</i>. [PDF]
iScienceZheng JZ +4 more
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Progressive oxygenation of developing leaves directs morphogenesis. [PDF]
Sci AdvPanicucci G +9 more
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Physcomitrium patens Protoplasting and Protoplast Transfection
2022Protoplast production with the moss Physcomitrium (Physcomitrella) patens has a long and successful history. As a tool, it has not only been the base of reverse genetic studies covering research fields as diverse as development, metabolism, or gene network regulation but also allowed its development as a bioengineering platform for protein production ...
Charlot, Florence +3 more
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Folia Microbiologica, 1964
Vegetative cells ofBacillus megaterium formed protoplasts in a sucrose-stabilized medium under the influence of lysozyme. The protoplasts sporulated during subsequent incubation. The morphological properties, germination, resistance to u. v. irradiation and thermo-resistance of protoplast spores were the same as with normal cells.
J, STARKA, J, CASLAVSKA
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Vegetative cells ofBacillus megaterium formed protoplasts in a sucrose-stabilized medium under the influence of lysozyme. The protoplasts sporulated during subsequent incubation. The morphological properties, germination, resistance to u. v. irradiation and thermo-resistance of protoplast spores were the same as with normal cells.
J, STARKA, J, CASLAVSKA
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VACUOLES OF FUNGAL PROTOPLASTS
Canadian Journal of Microbiology, 1967Details are given of the conditions which allow vacuolar structures of Fusarium culmorum to be released after osmotic bursting of mycelial protoplasts. Steps in the release of vacuoles are shown. The composition of the suspending medium affects the formation, size, and number of the vacuoles seen in the protoplasts.
I, Garcia-Acha +2 more
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Protoplast regeneration and organogenesis from pea protoplasts
In Vitro Cellular & Developmental Biology, 1989Protoplasts of young Pisum Sativum L. seedlings from 7 different genotypes were isolated and regenerated to the callus stage. Germinating embryos were cultivated with cotyledons removed, thus avoiding intracellular starch accumulation in donor tissue.
Renate Lehminger-Mertens +1 more
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Lactobacillus protoplast transformation
Plasmid, 1987A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
L, Morelli +5 more
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Haploid protoplasts: pollen protoplasts
1996The experimental significance of haploid protoplasts requires no explanation, and their biological potential was well reviewed by Bajaj (1983) about 10 years ago. Since the totipotency of haploid cells of higher plants was first demonstrated by the production of haploids derived from pollen grains in anther culture (Guha and Maheshwari, 1964), many ...
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Nature, 1974
RESEARCH on fusion of cells of higher eukaryotes is in an advanced stage and excellent reviews have been published concerning fusion of both animal cells1–4 and plant protoplasts5–7. On the other hand, no information is available regarding the results of fusion of fungal protoplasts, in spite of its possibilities for basic and applied research. Here we
L, Ferenczy, F, Kevei, J, Zsolt
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RESEARCH on fusion of cells of higher eukaryotes is in an advanced stage and excellent reviews have been published concerning fusion of both animal cells1–4 and plant protoplasts5–7. On the other hand, no information is available regarding the results of fusion of fungal protoplasts, in spite of its possibilities for basic and applied research. Here we
L, Ferenczy, F, Kevei, J, Zsolt
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2003
It is now possible to regenerate plants from protoplasts of a wide range of species. As a result, genetic manipulation by protoplast fusion in vitro is now a realistic proposition. This area is exciting because protoplasts from different origins can be fused together to form new genome combinations that cannot be obtained by conventional means.
N, Fish, K, Lindsey, M G, Jones
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It is now possible to regenerate plants from protoplasts of a wide range of species. As a result, genetic manipulation by protoplast fusion in vitro is now a realistic proposition. This area is exciting because protoplasts from different origins can be fused together to form new genome combinations that cannot be obtained by conventional means.
N, Fish, K, Lindsey, M G, Jones
openaire +2 more sources