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Protoplast Isolation and Staining
2015The successful isolation of mesophyll protoplasts from plant species has become a versatile tool for in vivo imaging of subcellular structures. Taking advantage of the various cytochemical probes available, the subcellular localization of specific organelles can be visualized in live protoplasts.
Liu, MQ +6 more
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Wheat protoplast culture: embryogenic colony formation from protoplasts
Plant Cell Reports, 1988Wheat (Triticum aestivum L. cv Chinese Spring) protoplasts were isolated from immature embryos or embryogenic calli (3-4 weeks of culture on MS medium with 32 mg/1 dicamba) and cultured in R2 medium containing 2 mg/1 2,4-D by the nurse culture methods originally developed for rice protoplasts (Kyozuka et al. 1987). Protoplasts isolated from embryogenic
Y, Hayashi, K, Shimamoto
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Protoplast Fusion and Protoplast Transformation in Micromonospora Species
1983It is well known that Micromonospora species produce important antibiotics but very few genetic works have been done with them. The new genetic manipulations are more frequently applied in the recent years in the strain improvement programs of antibiotics producing microorganisms.
M. Szigetvári-Gabányi, M. Járai
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Bacterial protoplast fusion: Recombination in fused protoplasts of Streptomyces coelicolor
Molecular and General Genetics MGG, 1978Numerous recombinants arose when protoplasts of S. coelicolor were treated with polyethylene glycol and regenerated on non-selective solid medium. In six-factor crosses, recombination frequencies of more than 10% (up to 17%) were routinely observed. This recombination did not require either of the known sex factors, SCPI and SCP2.
D A, Hopwood, H M, Wright
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Transient Expression in Protoplasts
Cold Spring Harbor Protocols, 2007INTRODUCTIONThe study of transient gene expression provides a useful complement to the study of stably transformed plants. Transient assays offer a quick method of testing the effects of genes, using either phenotypic, molecular, or biochemical readouts.
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1985
In its strictest sense a viable protoplast is one capable of regeneration into a plant, a capacity that has been demonstrated for few species and, with a few exceptions, for none in the conifers and monocotyledonous plants. In such cases where whole plant regeneration is potentially possible, there is no single parameter of viability, or collection of ...
Bornman, Chris H, Bornman, Janet
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In its strictest sense a viable protoplast is one capable of regeneration into a plant, a capacity that has been demonstrated for few species and, with a few exceptions, for none in the conifers and monocotyledonous plants. In such cases where whole plant regeneration is potentially possible, there is no single parameter of viability, or collection of ...
Bornman, Chris H, Bornman, Janet
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Studies on Chlorella protoplasts
Planta, 1978Protoplasts of Chlorella saccharophila (Krüger) Nadson were obtained by cellulase digestion of the microfibrillar inner compount of the cell wall after the resistant outermost layer had been scratched with sea sand. The absence of the cell wall was demonstrated immunologically, electron microscopically and by staining, thus confirming the protoplastic ...
H G, Aach, S, Bartsch, V, Feyen
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Totipotency of tomato protoplasts
Theoretical and Applied Genetics, 1985An efficient and reliable protocol for tomato protoplast isolation, culture, and plant regeneration has been developed. Fourteen diverse cultivars were tested. Fertile plants were regenerated from all 14 cultivars without any modification in the protocol. Plating efficiency (percentage of the protoplasts that formed minicalli) of up to 50% was achieved.
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Plant Protoplast Fusion and Hybridization
1977For some time we have been exposed to popular articles describing the magic and more recently some of the potential terror which lies before us as scientists acquire sophisticated skills in the engineering of new genes. We hear also of the construction of new plants with unsurpassed qualities and with an inbuilt nitrogen fixing system.
Gamborg, Oluf L., Holl, F. B.
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