Results 101 to 110 of about 431,100 (179)

Slow growth and unstable ribosomal RNA lacking pseudouridine in mouse embryonic fibroblast cells expressing catalytically inactive dyskerin [PDF]

, 2013
Pseudouridine is the most abundant modified nucleotide in ribosomal RNA throughout eukaryotes and archaea but its role is not known. Here we produced mouse embryonic fibroblast cells expressing only catalytically inactive dyskerin, the pseudouridine ...
Gu, Bai-Wei, Mason, Philip J., Ge, Jingping, Fan, Jian-Meng, Bessler, Monica   +4 more
core   +1 more source

5‐Fluorouracil treatment represses pseudouridine‐containing miRNA export into extracellular vesicles

Journal of Extracellular Biology
5‐Fluorouracil (5‐FU) has been used for chemotherapy for colorectal and other cancers for over 50 years. The prevailing view of its mechanism of action is inhibition of thymidine synthase leading to defects in DNA replication and repair. However, 5‐FU is
Shimian Qu, Hannah M. Nelson, Xiao Liu, Yu Wang, Elizabeth M. Semler, Danielle L. Michell, Clark Massick, Jeffrey L. Franklin, John Karijolich, Alissa M. Weaver, Robert J. Coffey, Qi Liu, Kasey C. Vickers, James G. Patton   +13 more
doaj   +1 more source

The Products of 5-Fluorouridine by the Action of the Pseudouridine Synthase TruB Disfavor One Mechanism and Suggest Another

, 2016
The pseudouridine synthase TruB handles 5-fluorouridine in RNA as a substrate, converting it into two isomeric hydrated products. Unexpectedly, the two products differ not in the hydrated pyrimidine ring but in the pentose ring, which is epimerized to ...
Eugene G. Mueller (2177312), Edward J. Miracco (2177310)   +1 more
core   +1 more source

A comprehensive tRNA pseudouridine map uncovers targets dependent on human stand-alone pseudouridine synthases

Nature Cell Biology
Abstract Pseudouridine (Ψ) is one of the most abundant RNA modifications in human cells, introduced post-transcriptionally by pseudouridine synthases (PUS). Despite its prevalence, the biological functions of Ψ remain poorly understood, largely due to the limited knowledge linking specific PUS enzymes to their targets.
Xu, H, Kong, L, Li, M, Pisignano, G, Cheng, J, Feng, F, Mehdipour, P, Song, C-X   +7 more
openaire   +4 more sources

The Pseudouridine Synthases:  Revisiting a Mechanism That Seemed Settled

, 2016
RNA containing 5-fluorouridine, [f 5U]RNA, has been used as a mechanistic probe for the pseudouridine synthases, which convert uridine in RNA to its C-glycoside isomer, pseudouridine.
Murray V. Johnston (1484782), Christopher J. Spedaliere (2705572), Joy M. Ginter (2705575), Eugene G. Mueller (2177312)   +3 more
core   +1 more source

Functional and Structural Impact of Target Uridine Substitutions on the H/ACA Ribonucleoprotein Particle Pseudouridine Synthase,

, 2016
Box H/ACA ribonucleoprotein protein particles catalyze the majority of pseudouridylation in functional RNA. Different from stand alone pseudouridine synthases, the RNP pseudouridine synthase comprises multiple protein subunits and an RNA subunit ...
Jing Zhou (168494), Hong Li (20183), Bo Liang (450389)   +2 more
core   +1 more source

The Handling of the Mechanistic Probe 5-Fluorouridine by the Pseudouridine Synthase TruA and Its Consistency with the Handling of the Same Probe by the Pseudouridine Synthases TruB and RluA

, 2016
RNA containing 5-fluorouridine (F5U) had previously been used to examine the mechanism of the pseudouridine synthase TruA, formerly known as pseudouridine synthase I [Gu et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 14270−14275]. From that work, it was
Yizhou Xie (1677910), Junjun Chen (529954), Eugene G. Mueller (2177312), Marguerite K. McDonald (2235895), Edward J. Miracco (2177310)   +4 more
core   +1 more source

Detection of pseudouridine synthase activity with a fluorescence-based yeast reporter system [PDF]

, 2020
Pseudouridine is an isomer of uridine and a post-transcriptional RNA modification in all domains of life. Its formation is catalyzed by pseudouridine synthases functioning as standalone proteins or protein-RNA complexes called H/ACA small nucleolar ...
University of Lethbridge. Faculty of Arts and Science, Dueck, Elijah   +1 more
core  

Glucan synthase of Phytophthora sojae [PDF]

, 2002
Glucans, with the (1-3)-b-glucosidic linkage as major feature, are present in most of the higher plants, in many lower plants, as well as in microorganisms (Stone and Clarke, 1992). The synthesis of (1-3)-b-glucan in vivo is catalysed by the enzyme (1-3)-
Antelo, Luis
core   +1 more source

PUS7‐dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression

Clinical and Translational Medicine
Background RNA pseudouridylation is a critical post‐transcriptional modification that influences gene expression and impacts various biological functions.
Yongxia Chang, Hao Jin, Yun Cui, Feng Yang, Kanghua Chen, Wenjun Kuang, Chunxiao Huo, Zhangqi Xu, Ya Li, Aifu Lin, Bo Yang, Wei Liu, Shanshan Xie, Tianhua Zhou   +13 more
doaj   +1 more source