Results 141 to 150 of about 3,085 (163)
Detection and quantification of RNA 2′-O-methylation and pseudouridylation [PDF]
Methods, 2016 RNA-guided RNA modification is a naturally occurring process that introduces 2'-O-methylation and pseudouridylation into rRNA, spliceosomal snRNA and several other types of RNA. The Box C/D ribonucleoproteins (RNP) and Box H/ACA RNP, each containing one unique guide RNA (Box C/D RNA or Box H/ACA RNA) and a set of core proteins, are responsible for 2'-O-
Chao Huang +2 more
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Rewriting the message: Harnessing RNA pseudouridylation to tackle disease
Molecular Cell, 2023Adachi et al.1 and Song et al.2 demonstrate the feasibility of engineering pseudouridylation at specific sites and its utility to correct disease-causing premature termination codons (PTCs) in human cells.
Matias Montes, Nicole M. Martínez
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ON THE BIOSYNTHESIS OF PSEUDOURIDINE AND OF PSEUDOURIDYLIC ACID IN AGROBACTERIUM TUMEFACIENS
Canadian Journal of Biochemistry, 1966Crude extracts prepared from the plant tumor inducing organism Agrobacterium tumefaciens were shown to convert uracil and D-ribose-5-phosphate to pseudouridine in stoichiometric amounts. The addition of the nucleotidase inhibitor sodium arsenate altered the course of the reactions involved in such a way that pseudouridylic acid became the product ...
T, Suzuki, R M, Hochster
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Pseudouridylation: A new player in co-transcriptional splicing regulation
Molecular Cell, 2022Martinez et al. (2022) uncovered a novel function for the most abundant modified nucleoside in RNA. The study shows that uridines at splice sites and splicing regulatory motifs in the pre-mRNA may be converted to pseudouridine during transcription and impact splicing decisions.
Rui, Sousa-Luís, Maria, Carmo-Fonseca
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Pseudouridylation-mediated gene expression modulation
Biochemical JournalRNA-guided pseudouridylation, a widespread post-transcriptional RNA modification, has recently gained recognition for its role in cellular processes such as pre-mRNA splicing and the modulation of premature termination codon (PTC) readthrough. This review provides insights into its mechanisms, functions, and potential therapeutic applications.
Jonathan L. Chen +4 more
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Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome
Nature Chemical Biology, 2015Pseudouridine (Ψ) is the most abundant post-transcriptional RNA modification, yet little is known about its prevalence, mechanism and function in mRNA. Here, we performed quantitative MS analysis and show that Ψ is much more prevalent (Ψ/U ratio ∼0.2-0.6%) in mammalian mRNA than previously believed. We developed N3-CMC-enriched pseudouridine sequencing
Xiaoyu, Li +6 more
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Quantitative profiling of pseudouridylation landscape in the human transcriptome
2022Abstract Pseudouridine (Ψ) is an abundant post-transcriptional RNA modification in ncRNA and mRNA. However, transcriptome-wide measurement of individual Ψ sites remains unaddressed. Here, we develop “PRAISE”, via selective chemical labeling of Ψ by bisulfite to induce nucleotide deletion signature during reverse transcription, to ...
Meiling Zhang +7 more
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Studies on pseudouridylic acid synthetase from various sources
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971Abstract Pseudouridylic acid synthetase (ΨMP-synthetase) was investigated in various tissues. 1. 1. Normal tobacco stem tissue contains ΨMP-synthetase which elutes from a DEAE-cellulose column in 0–0.1 M NaCl. 2. 2. Tobacco stem tissue transformed with Agrobacterium tumefaciens contains a ΨMP-synthetase that also elutes from the DEAE ...
T, Matsushita, F F, Davis
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Effects of Pseudouridylation on tRNA Hydration and Dynamics: a Theoretical Approach
2014This chapter focuses on the structural implications resulting from the occurrence of this residue in tRNA and also presents an overview of the tRNA pseudouridylation sites. The principle of molecular dynamics (MD) resides in the numerical integration of the Newtonian equations of motion. The potential energy function contains several terms that account
Auffinger, Pascal, Westhof, Eric
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Targeted pseudouridylation: An approach for suppressing nonsense mutations in disease genes
Molecular Cell, 2023Pedro Morais, Yi-Tao Yu, Hironori Adachi
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