The purine nucleoside phosphorylase pnp-1 regulates epithelial cell resistance to infection in C. elegans. [PDF]
PLoS Pathogens, 2021 Intestinal epithelial cells are subject to attack by a diverse array of microbes, including intracellular as well as extracellular pathogens. While defense in epithelial cells can be triggered by pattern recognition receptor-mediated detection of microbe-
Eillen Tecle +5 more
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Purine nucleoside phosphorylase enables dual metabolic checkpoints that prevent T cell immunodeficiency and TLR7-associated autoimmunity [PDF]
The Journal of Clinical Investigation, 2022 Purine nucleoside phosphorylase (PNP) enables the breakdown and recycling of guanine nucleosides. PNP insufficiency in humans is paradoxically associated with both immunodeficiency and autoimmunity, but the mechanistic basis for these outcomes is ...
Evan R. Abt +24 more
doaj +3 more sources
Efficient Synthesis of Purine Nucleoside Analogs by a New Trimeric Purine Nucleoside Phosphorylase from Aneurinibacillus migulanus AM007 [PDF]
Molecules, 2019 Purine nucleoside phosphorylases (PNPs) are promising biocatalysts for the synthesis of purine nucleoside analogs. Although a number of PNPs have been reported, the development of highly efficient enzymes for industrial applications is still in high ...
Gaofei Liu +4 more
doaj +3 more sources
Purine nucleoside phosphorylase inhibition ameliorates age-associated lower urinary tract dysfunctions [PDF]
JCI Insight, 2020 In the aging population, lower urinary tract (LUT) dysfunction is common and often leads to storage and voiding difficulties classified into overlapping symptom syndromes.
Lori A. Birder +12 more
doaj +3 more sources
Partial Purine Nucleoside Phosphorylase Deficiency Helps Determine Minimal Activity Required for Immune and Neurological Development [PDF]
Frontiers in Immunology, 2020 Introduction: Complete or near complete absence of the purine nucleoside phosphorylase (PNP) enzyme causes a profound T cell immunodeficiency and neurological abnormalities that are often lethal in infancy and early childhood.
Eyal Grunebaum +5 more
doaj +3 more sources
Connecting Conformational Motions to Rapid Dynamics in Human Purine Nucleoside Phosphorylase. [PDF]
J Phys Chem B, 2023 The influence of protein motions on enzyme catalysis remains a topic of active discussion. Protein motions occur across a variety of time scales, from vibrational fluctuations in femtoseconds, to collective motions in milliseconds.
Frost CF +3 more
europepmc +2 more sources
Synthesis of New 5′-Norcarbocyclic Aza/Deaza Purine Fleximers - Noncompetitive Inhibitors of E.coli Purine Nucleoside Phosphorylase [PDF]
Frontiers in Chemistry, 2022 A new series of flexible 5′-norcarbocyclic aza/deaza-purine nucleoside analogs were synthesized from 6-oxybicyclo[3.1.0.]hex-2-ene and pyrazole-containing fleximer analogs of heterocyclic bases using the Trost procedure.
Anastasia Khandazhinskaya +7 more
doaj +2 more sources
Early Diagnosis and Treatment of Purine Nucleoside Phosphorylase (PNP) Deficiency through TREC-Based Newborn Screening [PDF]
International Journal of Neonatal Screening, 2021 Purine nucleoside phosphorylase (PNP) deficiency is a rare inherited disorder, resulting in severe combined immunodeficiency. To date, PNP deficiency has been detected in newborn screening only through the use of liquid chromatography tandem mass ...
Andrea Martín-Nalda +13 more
doaj +2 more sources
Polymorphisme de la purine nucléoside phosphorylase érythrocytaire bovine [PDF]
Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux, 1984 La distribution de variants électrophorétiques de la purine nucléoside phosphorylase (N.P.) contrôlée par deux allèles NP.H et NP.L a été étudiée sur des hémolysats bovins appartenant à des populations autochtones de zébus ou de taurins (race Baoulé) et ...
R. Quéval, L. Bambara
doaj +3 more sources
Science Translational Medicine, 2019 A cellular thermal shift assay (CETSA) protocol identifies and resolves antimalarial drug targets in P. falciparum. Defining drug targets in malaria Different classes of malaria drugs have been used for decades, even though the mechanisms of action have ...
Jerzy Michal Dziekan, Han Yu, Dan Chen
exaly +2 more sources