Results 31 to 40 of about 24,425 (290)

8‐Aminoguanine Induces Diuresis, Natriuresis, and Glucosuria by Inhibiting Purine Nucleoside Phosphorylase and Reduces Potassium Excretion by Inhibiting Rac1 [PDF]

Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 2018
Background 8‐Aminoguanosine and 8‐aminoguanine are K+‐sparing natriuretics that increase glucose excretion. Most effects of 8‐aminoguanosine are due to its metabolism to 8‐aminoguanine.
Edwin K. Jackson, Zaichuan Mi, Thomas R. Kleyman, Dongmei Cheng   +3 more
doaj   +2 more sources

Cloning and expression of recombinant purine nucleoside phosphorylase in the methylotrophic yeast Pichia pastoris

Journal of Advanced Biotechnology and Experimental Therapeutics, 2023
Purine Nucleoside Phosphorylase (PNP) is an enzyme involved in biosynthetic pathway of purine nucleosides. Purine nucleoside phosphorylase catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides to form the purine base and ...
Jaloliddin Abdurakhmanov, Sobirdjan Sasmakov, Shukhrat Khasanov, Oybek Ashirov, Farkhod Eshboev, Khayotjon Dolimov, Saodat Gaynazarova, Tulkin Sadullaev, Oygul Yarilkaganova, Khusan Nasriddinov, Asadali Baymirzaev, Artyom Makhnyov, Shakhnoz Azimova   +12 more
doaj   +1 more source

Genetic resistance to purine nucleoside phosphorylase inhibition in Plasmodium falciparum. [PDF]

Proc Natl Acad Sci U S A, 2018
Significance Hypoxanthine salvage is essential for nucleic acid synthesis in Plasmodium falciparum but not in humans. Hypoxanthine production in P. falciparum can be blocked by DADMe-Immucillin-G (DADMe-ImmG), a transition-state analog inhibitor of ...
Ducati RG, Namanja-Magliano HA, Harijan RK, Fajardo JE, Fiser A, Daily JP, Schramm VL.   +6 more
europepmc   +2 more sources

Investigating the Vital Role of the Identified Abietic Acid from Helianthus annuus L. Calathide Extract against Hyperuricemia via Human Embryonic Kidney 293T Cell Model

Molecules, 2023
To explore the anti-hyperuricemia components in sunflower (Helianthus annuus L.) calathide extract (SCE), we identified abietic acid (AA) via liquid chromatography–mass spectrometry and found an excellent inhibitor of xanthine oxidase (IC50 = 10.60 µM ...
Huining Dai, Xiao Xu, Wannan Li, Xueqi Fu, Weiwei Han, Guodong Li   +5 more
doaj   +1 more source

Some inhibitors of purine nucleoside phosphorylase [PDF]

Biomeditsinskaya Khimiya, 2011
Purine nucleoside phosphorylase (PNP) catalyzes reversible phosphorolysis of purine deoxy- and ribonucleosides with formation (d)Rib-1-P and corresponding bases. PNP plays a leading role in the cell metabolism of nucleosides and nucleotides, as well as in maintaining the immune status of an organism.
J. I. Akopian, L. H. Pogosian, Gazariants Mg, Mkrtchian Zs, Nersesova Ls   +4 more
openaire   +3 more sources

Inverse enzyme isotope effects in human purine nucleoside phosphorylase with heavy asparagine labels. [PDF]

Proc Natl Acad Sci U S A, 2018
Significance Enzymes achieve catalytic efficiency by optimizing contacts between reactants and catalytic site amino acids. The transition state forms rarely, with a lifetime of a few femtoseconds.
Harijan RK, Zoi I, Antoniou D, Schwartz SD, Schramm VL.   +4 more
europepmc   +2 more sources

Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations [PDF]

, 2020
The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in ...
Giessmann, Robert T., Kaspar, Felix, Klare, Hendrik F. T., Kurreck, Jens, Neubauer, Peter, Paulick, Katharina, Röhrs, Viola, Wagner, Anke, Westarp, Sarah, Yehia, Heba   +9 more
core   +1 more source

Thermodynamic reaction control of nucleoside phosphorolysis [PDF]

, 2020
Nucleoside analogs represent a class of important drugs for cancer and antiviral treatments. Nucleoside phosphorylases (NPases) catalyze the phosphorolysis of nucleosides and are widely employed for the synthesis of pentose‐1‐phosphates and nucleoside ...
Giessmann, Robert T., Gimpel, Matthias, Kaspar, Felix, Neubauer, Peter, Wagner, Anke   +4 more
core   +1 more source

Purine Nucleoside Phosphorylase Deficiency [PDF]

Arthritis & Rheumatism, 1979
AbstractThe biochemical features of two families with purine nucleoside phosphorylase deficiency are compared. Laboratory studies and an evaluation of kinetic and physical properties of erythrocyte purine nucleoside phosphorylase give evidence that a) the degree of abnormality in uric acid and nucleoside concentrations in plasma and urine reflect the ...
Irving H. Fox, Robert L. Wortmann, Edwin Mejias, William J. Arnold, Catherine M. Andres, Erwin W. Dr. Gelfand, Janice Kaminska, Kenneth Rich   +7 more
openaire   +2 more sources

PNP inhibitors selectively kill cancer cells lacking SAMHD1

Molecular & Cellular Oncology, 2020
Purine nucleoside phosphorylase inhibitors (PNP-Is) were developed to ablate transformed lymphocytes. However, only some patients with leukemia benefit from PNP-Is. We provide a molecular explanation: the deoxyribonucleoside triphosphate (dNTP) hydrolase
Tamara Davenne, Jan Rehwinkel
doaj   +1 more source