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Assay of pyridoxal-5'-phosphate, pyridoxal and pyridoxic acid in biological material.
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition, 1990The two vitamin B6-vitamers having an aldehyde function are oxidised to the corresponding acids and subjected to an HPLC separation on an RP 18 phase with a solvent consisting of 5% methanol in phosphate buffer at pH 3.5. The detection is carried out by fluorometry with excitation at 318 nm and emission at 418 nm.
D, Hess, J P, Vuilleumier
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Inactivation of microbial pyridoxal kinase by pyridoxal.
Acta vitaminologica et enzymologica, 1982Pyridoxal kinase from Escherichia coli and bakers' yeast was inactivated by pyridoxal while the enzyme from rat and pig brain was not. The inactivation of the enzyme purified from E. coli was reversible and was rendered irreversible by the reduction with NaBH4.
Y, Furukawa, R, Yamada, A, Iwashima
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Modification of intracellular hemoglobin with pyridoxal and pyridoxal 5'-phosphate.
Blood cells, 1983The aldehyde forms of vitamin B6, pyridoxal and pyridoxal 5'-phosphate (PLP) have aroused interest as antisickling agents because of their ability to modify hemoglobin (Hb) and their low toxicity. To study their rate of formation and stability inside red cells, pyridoxal-Hb and PLP-Hb were measured in lysates from treated normal and sickle erythrocytes
J A, Kark +5 more
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Biomimetic Chirality Sensing with Pyridoxal-5'-phosphate.
Journal of the American Chemical Society, 2017Samantha L. Pilicer +3 more
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REACTION OF PYRIDOXAL AND PYRIDOXAL‐5‐PHOSPHATE WITH PROTEINS.
Journal of Food Science, 1972V. SRNCOVÁ, J. DAVÍDEK
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Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Annual Review of Biochemistry, 2003A. C. Eliot, J. Kirsch
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