Results 161 to 170 of about 7,956 (190)

Detection of the cofactor pyrroloquinoline quinone

Analytical Biochemistry, 1987
In order to demonstrate the presence or absence of a pyrroloquinoline quinone (PQQ) synthesizing capacity in microorganisms, we have found that media and equipment must be treated to remove contaminating PQQ. Procedures are described which appear to be effective for that purpose.
M A, van Kleef, P, Dokter, A C, Mulder, J A, Duine   +3 more
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Pyrroloquinoline Quinone: A Novel Cofactor

1989
Publisher Summary This chapter discusses the properties, distribution, and biosynthesis of pyrroloquinoline quinine (PQQ). PQQ is an essential cofactor for several key enzymes in physiological processes in mammals. Development of inhibitors specifically directed to PQQ might reveal the role of PQQ and quinoproteins, but also that of vitamin B, and ...
J A, Duine, J A, Jongejan
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A Periplasmic Binding Protein for Pyrroloquinoline Quinone

Biochemistry, 2019
Pyrroloquinoline quinone (PQQ) is an essential redox cofactor in bacterial calcium- and lanthanide-dependent alcohol dehydrogenases. Although certain bacteria are known to synthesize and secrete PQQ, little is known about trafficking of this cofactor within and between cells.
Jackson V. Ho, Joseph A. Cotruvo
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Method of enzymatic determination of pyrroloquinoline quinone

Analytical Biochemistry, 1985
An improved enzymatic method for the determination of pyrroloquinoline quinone, a novel prosthetic group of some important oxidoreductases, has been developed with cytoplasmic membrane of Escherichia coli K-12, in which D-glucose dehydrogenase (EC 1.1.99.17) was completely resolved to apo-enzyme by EDTA treatment.
M, Ameyama, M, Nonobe, E, Shinagawa, K, Matsushita, O, Adachi   +4 more
openaire   +2 more sources

Pyrroloquinoline Quinone (PQQ) and Quinoprotein Enzymes

Antioxidants & Redox Signaling, 2001
This review summarises the characteristics, identification, and measurement of pyrroloquinoline quinone, the prosthetic group of bacterial quinoprotein dehydrogenases whose structures, mechanisms, and electron transport functions are described in detail. Type I alcohol dehydrogenase includes the "classic" methanol dehydrogenase; its x-ray structure and
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Biochemical and Physiological Functions of Pyrroloquinoline Quinone

1991
Publisher Summary This chapter discusses the biochemical and physiological functions of pyrroloquinoline quinone (PQQ). Quinoproteins are distributed widely among prokaryotic and eukaryotic organisms. The prosthetic group of copper-containing amine oxidases from mammals, plants, and microorganisms have been identified as PQQ bound covalently to the ...
M, Ameyama, K, Matsushita, E, Shinagawa, O, Adachi   +3 more
openaire   +2 more sources

Pyrroloquinoline quinone-dependent dehydrogenases of acetic acid bacteria

Applied Microbiology and Biotechnology, 2018
Pyrroloquinoline quinone (PQQ)-dependent dehydrogenases (quinoproteins) of acetic acid bacteria (AAB), such as the membrane-bound alcohol dehydrogenase (ADH) and the membrane-bound glucose dehydrogenase, contain PQQ as the prosthetic group. Most of them are located on the periplasmic surface of the cytoplasmic membrane, and function as primary ...
Minenosuke Matsutani, Toshiharu Yakushi
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Nutritional Essentiality of Pyrroloquinoline Quinone

1989
A chemically defined diet, based on synthetic amino acids, was fed to rats and mice. The control diet (+PQQ) was fortified with pyrroloquinoline quinone (PQQ) at 875 ug/kg. The deficient diet (-PQQ) contained no detectable PQQ. The -PQQ diet was fed to pregnant rats and, in later experiments, to mice throughout gestation. Newborn pups remained with the
Robert Rucker, John Killgore, Lisa Duich, Nadia Romero-Chapman, Carsten Smidt, Donald Tinker   +5 more
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Determination of pyrroloquinoline quinone by capillary zone electrophoresis

Journal of Chromatography B: Biomedical Sciences and Applications, 2000
A new method for the determination of pyrroloquinoline quinone by capillary zone electrophoresis has been developed. Separation conditions have been optimised with the respect to different parameters including pH and ionic strength of the background electrolyte, separation voltage and temperature of the capillary.
Z, Glatz, M, Moravcová, O, Janiczek
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Extractions of pyrroloquinoline quinone from crude biological samples

Life Sciences, 1990
The best conditions for extractions of free pyrroloquinoline quinone (PQQ) from crude biological samples were investigated with various organic solvents and Sep-Pak C18 cartridges. PQQ was measured with use of its native fluorescence in aqueous solution.
O, Suzuki, T, Kumazawa, H, Seno, T, Urakami, T, Matsumoto   +4 more
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