Results 61 to 70 of about 1,475 (148)
Site-specific Effects of Lysine Acetylation on Aminoacyl-tRNA Synthetase [PDF]
, 2021 Aminoacyl-tRNA synthetases (AARSs) are an ancient and highly conserved family of enzymes which can catalyze a two-steps aminoacylation reaction to charge tRNAs with their cognate amino acids, thus playing crucial roles in ribosomal protein synthesis ...
Chen, Hao
core +1 more source
Plant Biotechnology Journal, Volume 24, Issue 1, Page 31-43, January 2026.Summary In plant molecular farming, recombinant products include traditional protein targets, such as antibodies, antigens, and enzymes, but also increasingly refers to high‐value peptides, and small molecules produced by heterologous enzymatic pathways.
Maxim D. Harding, Frank Sainsbury
wiley +1 more source
Internal Ubiquitin Electrophiles for Covalent Trapping and Inhibition of Deubiquitinases
ChemBioChem, Volume 26, Issue 22, November 17, 2025.GA: Genetically encoded internal electrophiles in ubiquitin (Ub) enable the profiling of multivalent interaction interfaces, addressing a key limitation of current Ub‐based activity‐based probes. In this study, these probes capture a Ub–deubiquitinase interaction with minimal structural perturbation and serve as selective diUb probes that covalently ...
Nipuni M. Pannala +7 more
wiley +1 more source
Systematic Evolution and Study of UAGN Decoding tRNAs in a Genomically Recoded Bacteria [PDF]
, 2016 We report the first systematic evolution and study of tRNA variants that are able to read a set of UAGN (N = A, G, U, C) codons in a genomically recoded E. coli strain that lacks any endogenous in-frame UAGN sequences and release factor 1.
Cerny, Ronald +4 more
core +4 more sources
Genetic code expansion in \u3ci\u3ePseudomonas putida\u3c/i\u3e KT2440 [PDF]
, 2022 Pseudomonas putida KT2440 is an emerging microbial chassis for bio-based chemical production from renewable feedstocks and environmental bioremediation.
Chen, Yan +5 more
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ChemCatChem, Volume 17, Issue 20, October 20, 2025.Thioxanthone is an efficient visible‐light photosensitizer and facilitates reactions that are not known from natural enzymes. It is now possible to incorporate thioxanthone as a noncanonical amino acid (thioX) into proteins by an engineered amino acyl tRNA synthetase.
Marco Seifert +3 more
wiley +1 more source
The Engineering and Optimization of Pyrrolysyl-Synthetase as a Tool for Noncanonical Amino Acid Incorporation [PDF]
, 2018 Since the discovery of pyrrolysine as the 22nd amino acid, the field of chemical biology has expanded tremendously with important developments made in genetic noncanonical amino acid (ncAA) incorporation based on the pyrrolysine incorporation machinery ...
Sharma, Vangmayee
core +1 more source
Noncanonical Amino Acid Incorporated Photocaged Cysteine for Multiple Cysteine Based ACPL Approach [PDF]
, 2022 Today, proteins and protein functionality are a major focus of biological chemistry. Every synthesized protein has a unique and innate biological function, understanding these functions and their application has been at the forefront of scientific ...
Wilson, Jaye A
core
Rational design of a covalent ACE2 decoy receptor that broadly neutralizes SARS‐CoV‐2 variants
Protein Science, Volume 34, Issue 10, October 2025.Abstract The ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and the emergence of variants that evade existing vaccines and antibody therapies necessitate novel, potent, and broad‐spectrum antiviral strategies. Enhancing therapeutic proteins with additional covalent binding capabilities, such as minibinders and ...
Nobumasa Hino +18 more
wiley +1 more source
, 2023 Abstract Using orthogonal translation systems (OTSs) is the most efficient way to produce unnatural proteins by adding non-canonical amino acids (ncAAs) to the genetic code. In the quest to expand substrate specificity the conventional approach begins with a (hyper-)stable enzyme capable of withstanding the structural changes resulting from ...
Nediljko Budisa +3 more
openaire +1 more source