Results 21 to 30 of about 68,490 (304)
Metabolic checkpoint of ferroptosis resistance
Molecular & Cellular Oncology, 2021 The metabolic checkpoint of ferroptosis remains obscure. We find that glucose favors system xc− inhibitor-induced ferroptosis by activating pyruvate oxidation, thereby promoting fatty acid synthesis and subsequent lipid peroxidation.
Jiao Liu, Rui Kang, Daolin Tang
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Frontiers in Plant Science, 2012 The mitochondrial pyruvate dehydrogenase complex is regulated by reversible seryl-phosphorylation of the E1α subunit by a dedicated, intrinsic kinase. The phospho-complex is reactivated when dephosphorylated by an intrinsic PP2C-type protein phosphatase.
Nagib eAhsan +5 more
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Regulation of mammalian pyruvate dehydrogenase kinase [PDF]
FEBS Letters, 1997 It is generally believed that mammalian pyruvate dehydrogenase kinase is a heterodimer consisting of catalytic and regulatory subunits. However, the contribution of the two subunits to the kinase‐mediated signal transduction has remained undefined. In the present study recombinant components of mammalian pyruvate dehydrogenase complex were employed in ...
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Regulation of heart muscle pyruvate dehydrogenase kinase [PDF]
Biochemical Journal, 1974 1. The activity of pig heart pyruvate dehydrogenase kinase was assayed by the incorporation of [32P]phosphate from [γ-32P]ATP into the dehydrogenase complex. There was a very close correlation between this incorporation and the loss of pyruvate dehydrogenase activity with all preparations studied. 2.
Ronald H. Cooper +2 more
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Allosteric Coupling in Pyruvate Dehydrogenase Kinase 2 [PDF]
Biochemistry, 2008 Mitochondrial pyruvate dehydrogenase kinase 2 (PDHK2) phosphorylates the pyruvate dehydrogenase multienzyme complex (PDC) and thereby controls the rate of oxidative decarboxylation of pyruvate. The activity of PDHK2 is regulated by a variety of metabolites such as pyruvate, NAD (+), NADH, CoA, and acetyl-CoA.
Alla Klyuyeva +2 more
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Purification and partial characterization of rat liver pyruvate dehydrogenase kinase activator protein (free pyruvate dehydrogenase kinase) [PDF]
FEBS Letters, 1992 Rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP), a free PDH kinase readily separable from PDH complex and its intrinsic kinase, has been purified to apparent homogeneity from liver mitochondria of fed and 48‐h starved rats. On SDS‐PAGE an apparently single band of M, 45 kDa was obtained.
Alan L. Kerbey +3 more
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Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle [PDF]
The Journal of Physiology, 2004 This study examined the effects of short‐ and long‐term aerobic training on the stable up‐regulation of pyruvate dehydrogenase (PDH) and PDH kinase (PDK) in human skeletal muscle. We hypothesized that 8 weeks, but not 1 week, of aerobic training would increase total PDH (PDHt) and PDK activities compared to pretraining, and this would be detectable at ...
Sandra J. Peters +4 more
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Warburg effect hypothesis in autism Spectrum disorders
Molecular Brain, 2018 Autism spectrum disorder (ASD) is a neurodevelopmental disease which is characterized by a deficit in social interactions and communication with repetitive and restrictive behavior. In altered cells, metabolic enzymes are modified by the dysregulation of
Alexandre Vallée, Jean-Noël Vallée
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A simple and reliable method for determination of optimum pH in coupled enzyme assays
BioTechniques, 2020 Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes.
Lee Bowman +7 more
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Detecting de novo Hepatic Ketogenesis Using Hyperpolarized [2-13C] Pyruvate
Frontiers in Physiology, 2022 The role of ketones in metabolic health has progressed over the past two decades, moving from what was perceived as a simple byproduct of fatty acid oxidation to a central player in a multiplicity of disease states. Previous work with hyperpolarized (HP)
Mukundan Ragavan +3 more
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