Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits [PDF]
, 2020 The success of fundamental and applied nucleic acid (NA) research depends on NA purity, but obtaining pure NAs from raw, unprocessed samples is challenging.
Ismagilov, Rustem F. +2 more
core
Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
bioRxiv, 2020 The current RT-qPCR assay recommended for SARS-CoV-2 testing in the United States requires analysis of three genomic targets per sample: two viral and one host.
Eriko Kudo +21 more
semanticscholar +1 more source
Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology
Molecular Oncology, EarlyView.Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts +8 more
wiley +1 more source
Digital PCR as a tool to measure HIV persistence [PDF]
, 2018 Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped.
Bosman, Kobus +4 more
core +1 more source
Establishment of a murine model of congenital toxoplasmosis and validation of a qPCR assay to assess the parasite load in maternal and fetal tissues [PDF]
, 2023 Jéssica S. Souza +6 more
openalex +1 more source
A 5-min RNA preparation method for COVID-19 detection with RT-qPCR
medRxiv, 2020 RNA extraction has become a bottleneck for detection of COVID-19, in part because of reagent shortages. We present here a rapid protocol that circumvents the need for RNA extraction that is compatible with RT-qPCR-based detection methods.
Alim Ladha +4 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.A‐to‐I editing of miRNAs, particularly miR‐200b‐3p, contributes to HGSOC progression by enhancing cancer cell proliferation, migration and 3D growth. The edited form is linked to poorer patient survival and the identification of novel molecular targets.
Magdalena Niemira +14 more
wiley +1 more source
Benchmarking of RNA-sequencing analysis workflows using whole-transcriptome RT-qPCR expression data
Scientific Reports, 2017 RNA-sequencing has become the gold standard for whole-transcriptome gene expression quantification. Multiple algorithms have been developed to derive gene counts from sequencing reads.
C. Everaert +6 more
semanticscholar +1 more source
Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids
Molecular Oncology, EarlyView.This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici +8 more
wiley +1 more source
Discriminating between viable and membrane-damaged cells of the plant pathogen Xylella fastidiosa. [PDF]
, 2019 Xylella fastidiosa is a plant pathogenic bacterium with devastating consequences to several crops of economic importance across the world. While this pathogen has been studied for over a century in the United States, several aspects of its biology remain
Almeida, Rodrigo +5 more
core +4 more sources