Results 1 to 10 of about 1,617,447 (322)

Identification of Suitable Reference Genes for qRT-PCR Normalization in Kiwifruit

open access: yesHorticulturae, 2022
Reference genes are used for the correction of qRT-PCR data, and it is necessary to investigate the optimum reference gene under certain conditions. The expression levels of seven traditional reference genes ACT1, ACT2, GAPDH, 18S rRNA, UBQ, TUB and CYP ...
Yuanjie Zhou   +11 more
doaj   +2 more sources

A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity

open access: yesMicroorganisms, 2020
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to limit the spread of the global pandemic we are facing.
Sara Petrillo   +11 more
doaj   +2 more sources

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets

open access: yesEmerging Microbes and Infections, 2020
Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method.
Xinjin Liu   +13 more
doaj   +2 more sources

A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses

open access: yesVeterinary Sciences, 2022
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) are four identified porcine enteric coronaviruses.
Hongjin Zhou   +9 more
doaj   +2 more sources

Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence [PDF]

open access: yesThe Plant Pathology Journal, 2018
Strawberry Fusarium wilt disease, caused by Fusarium oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades in
Sung Won Hong   +5 more
doaj   +3 more sources

Evaluation of droplet digital qRT-PCR (dd qRT-PCR) for quantification of SARS CoV-2 RNA in stool and urine specimens of COVID-19 patients [PDF]

open access: yesFrontiers in Medicine, 2023
IntroductionThere have been a few reports of viral load detection in stool and urine samples of patients with coronavirus disease 2019 (COVID-19), and the transmission of the virus through faecal oral route.
Manohar Shinde   +4 more
doaj   +2 more sources

Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.

open access: yesPLoS ONE, 2020
To accurately evaluate expression levels of target genes, stable internal reference genes is required for normalization of quantitative real-time PCR (qRT-PCR) data.
Xu Su   +11 more
doaj   +2 more sources

No control genes required: Bayesian analysis of qRT-PCR data.

open access: yesPLoS ONE, 2013
BackgroundModel-based analysis of data from quantitative reverse-transcription PCR (qRT-PCR) is potentially more powerful and versatile than traditional methods.
Mikhail V Matz   +2 more
doaj   +5 more sources

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus [PDF]

open access: yesFrontiers in Microbiology
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus.
Lulu Li, Xiaofan Guo, Shouming Wang
doaj   +2 more sources

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