Results 1 to 10 of about 491,650 (139)

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus [PDF]

Frontiers in Microbiology
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus.
Lulu Li, Xiaofan Guo, Shouming Wang
doaj   +2 more sources

Evaluation of droplet digital qRT-PCR (dd qRT-PCR) for quantification of SARS CoV-2 RNA in stool and urine specimens of COVID-19 patients

Frontiers in Medicine, 2023
IntroductionThere have been a few reports of viral load detection in stool and urine samples of patients with coronavirus disease 2019 (COVID-19), and the transmission of the virus through faecal oral route.
Manohar Shinde, Mallika Lavania, Jatin Rawal, Nutan Chavan, Pooja Shinde   +4 more
doaj   +1 more source

A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity

Microorganisms, 2020
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to limit the spread of the global pandemic we are facing.
Sara Petrillo, Giovanna Carrà, Paolo Bottino, Elisa Zanotto, Maria Chiara De Santis, Jean Piero Margaria, Alessandro Giorgio, Giorgia Mandili, Miriam Martini, Rossana Cavallo, Davide Barberio, Fiorella Altruda   +11 more
doaj   +1 more source

An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5

Veterinary Sciences, 2023
A duplex real-time quantitative reverse transcription-polymerase chain reaction (dqRT-PCR) assay was successfully developed to simultaneously detect canine parainfluenza virus 5 (CPIV5) and a canine endogenous internal positive control (EIPC) in canine ...
Gyu-Tae Jeon, Hye-Ryung Kim, Yeun-Kyung Shin, Oh-Kyu Kwon, Hae-Eun Kang, Oh-Deog Kwon, Choi-Kyu Park   +6 more
doaj   +1 more source

Clinical Value of S-poly (T) plus Real-time PCR in Detection of Liver Cancer-related miR-199

Zhongliu Fangzhi Yanjiu, 2020
Objective To explore the advantages and disadvantages of S-poly(T) plus qRT-PCR and Poly (A) qRT-PCR methods in detecting peripheral blood serum miR-199.
WU Juan, ZHANG Yaqin, WU Yunbin, CHEN Min, YU Zhou, CAO Yingping   +5 more
doaj   +1 more source

Transcription analysis of apple fruit development using cDNA microarrays [PDF]

, 2009
The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding.
Costa, F., Gianfranceschi, L., Molthoff, J.W., Schouten, H.J., Soglio, V., Weemen-Hendriks, M.   +5 more
core   +8 more sources

Spit or Swab? The Diagnostic Accuracy of Saliva-Based Testing as a Sars-Cov-2 Surveillance Tool

Philippine Journal of Pathology, 2022
Background. Nasopharyngeal swab/oropharyngeal swab (NPS/OPS) qRT-PCR is the gold standard for detecting SARS-CoV-2. However, it has its own limitations including cost and invasiveness.
Raymundo Lo, Farrah Kristine Santiago, Grig Misiona, Melani Sionzon, Danielle Anne Gonong, Jill Jaime, Jan Roman Ayco, Aquiles Joseph Lira, Erik Francis Rarugal, Jon Carlo Crisostomo, Manuel Bautista Jr., Kenneth Chrysostom Ibarra, Eunice Jane Ciriaco, Arielle Trish Zamora, Kimberly Abundo, Emmylou Tarnate, Jonathan Nario, Jan Joseph Hernandez, Kathleen Mae Montes, Ma. Anabella Recio   +19 more
doaj   +1 more source

Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra, Chandrashekhar Raut, Neelam Prasad, J S Bhawalkar, Safia Farooqui, Shahzad Mirza, Veshal V Madan   +6 more
doaj   +1 more source

Development of reverse-transcription PCR techniques to analyse the density and sex ratio of gametocytes in genetically diverse Plasmodium chabaudi infections [PDF]

, 2007
We have developed cross-genotype and genotype-specific quantitative reverse-transcription PCR (qRT-PCR) assays to detect and quantify the number of parasites, transmission stages (gametocytes) and male gametocytes in blood stage Plasmodium chabaudi ...
Abdel-Wahab, Alano, Babiker, Bell, Cheeseman, Damien R. Drew, De Roode, De Roode, De Roode, Dye, Ferguson, Ferguson, Foster, Gardner, Gautret, Hamilton, Khan, Mackinnon, Nee, Paterson, Paul, Paul, Paul, Pinheiro, Ranford-Cartwright, Read, Read, Read, Reece, Reece, Reece, Sarah E. Reece, Schall, Schall, Schneider, Shutler, Sinden, Taylor, Taylor, Wargo, Wargo, West, West, West, West   +44 more
core   +1 more source

Preconditioning of mesenchymal stromal cells with low-intensity ultrasound: influence on chondrogenesis and directed SOX9 signaling pathways [PDF]

, 2020
Background: Continuous low-intensity ultrasound (cLIUS) facilitates the chondrogenic differentiation of human mesenchymal stromal cells (MSCs) in the absence of exogenously added transforming growth factor-beta (TGFβ) by upregulating the expression of ...
A Argentaro, A Augello, A Katsumi, A Woods, A Woods, AD Miller, AH Huang, B Yang, BH Choi, C Huang, C Matta, CA Simmons, CB Little, CJ O’Conor, CY Huang, D Schumann, DR Haudenschild, EM Darling, F Dell'Accio, G Coricor, H Sim, H-C Hsu, HJ Kwon, HJ Lee, HJ Lee, J Li, J Puetzer, JC Sng, JD Kisiday, JH Cui, JH Cui, JW Choi, K Ebisawa, K Pelttari, L Bian, Li Ling, LJ Ng, M Shakibaei, M Tsuda, MA Corrigan, MB Mueller, N Fahy, N Thelin, NP Whitney, P Müller, R Ogawa, RL Mauck, RM Delaine-Smith, RS Tigli, S Murakami, S Noriega, S Noriega, S Yamaguchi, SD Cook, SD Cook, T Furumatsu, T Guo, T Ito, T Juhasz, T Nishida, T Sanjukta Guha, TM Louw, V Lefebvre, V Lefebvre, W Huang, W Huang, Z Li, ZJ Zhang   +67 more
core   +2 more sources