Results 11 to 20 of about 491,749 (238)

FUS mutant human motoneurons display altered transcriptome and microRNA pathways with implications for ALS pathogenesis [PDF]

, 2017
The FUS gene has been linked to amyotrophic lateral sclerosis (ALS). FUS is a ubiquitous RNA-binding protein, and the mechanisms leading to selective motoneuron loss downstream of ALS-linked mutations are largely unknown.
Alfano, Vincenzo, Bozzoni, Irene, Colantoni, Alessio, DE SANTIS, Riccardo, DE TURRIS, Valeria, Peruzzi, Giovanna, Rosa, Alessandro, Santini, Laura   +7 more
core   +5 more sources

Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus

Veterinary Sciences, 2023
Atypical porcine pestivirus (APPV), a newly discovered virus, is associated with the type A-II congenital tremor (CT) in neonatal piglets. APPV distributes throughout the world and causes certain economic losses to the swine industry.
Huixin Liu, Kaichuang Shi, Shuping Feng, Yanwen Yin, Feng Long, Hongbin Si   +5 more
doaj   +1 more source

A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test [PDF]

, 2014
A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species.
De Craeye, Stéphane, Francart, Aurélie, Kalai, Michael, Lamoral, Sophie, Nazé, Florence, Suin, Vanessa, Van Gucht, Steven   +6 more
core   +4 more sources

Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Virology Journal, 2020
Background Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for clinical ...
Yang Zhang, Chunyang Dai, Huiyan Wang, Yong Gao, Tuantuan Li, Yan Fang, Zuojun Shen, Lichang Chen, Zhaowu Chen, Xuejun Ma, Ming Li   +10 more
doaj   +1 more source

Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens. [PDF]

, 2015
BackgroundTo determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.MethodsTen CLL specimens and five normal peripheral blood CD19+ B ...
A Ameur, A Choudhury, A Hidalgo, A Rosenwald, AS Kamiguti, C Haslinger, C Soo, C Trapnell, C Trapnell, CJ David, DC Gilby, E Moon, E Moravcikova, F Colotta, F Talab, FC Kelleher, Gwen Jordaan, H Dohner, H Jiang, J Eswaran, J Vilpo, JC Marioni, JJ Dongen van, JP Venables, K Inada, K Zen, L Chen, L Wang, L Yang, LQ Zhang, M Crespo, M Rehli, M Shinohara, M Sultan, Matteo Pelegrini, ML Hermiston, MW Pfaffl, N Chiorazzi, NA Twine, O Bermudez, P Guglielmi, P Kapranov, PG Ferreira, Phillipp Nham, Q Huang, RA Higgins, RI Fernando, RK Gandhirajan, RN Damle, Ryan T. Phan, S Anders, S Courtois, S Decker, S Falt, S Ibrahim, S Liu, S Ma, S Ren, S Shen, Sanjai Sharma, SM Russell, SP Shah, SR Gunn, T Ormsby, TJ Hamblin, U Klein, V Quesada, Wei Liao, XS Puente, Y Pawitan, Y Pekarsky, Y Yu, Z Wang   +72 more
core   +1 more source

Comparative analysis of the diagnostic performance of five commercial COVID-19 qRT PCR kits used in India

Scientific Reports, 2021
To meet the unprecedented requirement of diagnostic testing for SARS-CoV-2, a large number of diagnostic kits were authorized by concerned authorities for diagnostic use within a short period of time during the initial phases of the ongoing pandemic.
J. Singh, A. K. Yadav, A. Pakhare, P. Kulkarni, L. Lokhande, P. Soni, M. Dadheech, P. Gupta, N. Masarkar, A. K. Maurya, S. Nema, D. Biswas, S. Singh   +12 more
doaj   +1 more source

Serum microRNA array analysis identifies miR-140-3p, miR-33b-3p and miR-671-3p as potential osteoarthritis biomarkers involved in metabolic processes. [PDF]

, 2017
Background: MicroRNAs (miRNAs) in circulation have emerged as promising biomarkers. In this study, we aimed to identify a circulating miRNA signature for osteoarthritis (OA) patients and in combination with bioinformatics analysis to evaluate the utility
A Brazma, A Nan, A Okuhara, A Turchinovich, B Aryal, B Mayr, B Sharif, B Zhang, BM Bolstad, BM Kuhn, BM Mayr, C Beyer, C Chen, C Guay, C Taurino, CL Andersen, D Dlouha, D Iliopoulos, D Zhang, DT Felson, F Kostopoulou, FE Nicolas, G Dennis Jr, G Li, G Tardif, GD Miles, GR Sondag, GS Lien, H Causton, JB van Meurs, JD Storey, JD Storey, KC Vickers, L Qi, M Cross, M Parrizas, MB Goldring, MC Hochberg, MD Paraskevopoulou, MP Gantier, OF Laterza, Q Zhuo, R Gernapudi, RF Loeser, RI Gregory, S Kluzek, S Kumar, S Miyaki, T Thomou, TA Karlsen, VM Borgonio Cuadra, W Cleveland, WM Reichmann, X Chen, X Chen, X Tan, X Wang, Y Klipper-Aurbach, Y Zhang, YH Li   +59 more
core   +2 more sources

EVALUACIÓN DE LA EXPRESIÓN DE GENES IMPLICADOS EN LA BIOSÍNTESIS DE ALMIDÓN EN DIFERENTES VARIEDADES DE YUCA

Acta Biológica Colombiana, 2015
Las raíces almacenadoras de yuca representan una fuente importante de almidón. La ruta metabólica del almidón ha sido reconstruida recientemente en yuca gracias a la liberación de la secuencia completa de su genoma. En este estudio se evaluó la expresión
Simón Pedro Cortés Sierra, Paul Chavarriaga, Hernán Ceballos, Camilo Ernesto López Carrascal   +3 more
doaj   +1 more source

The biogenesis pathway of tRNA-derived piRNAs in Bombyx germ cells. [PDF]

, 2017
Transfer RNAs (tRNAs) function in translational machinery and further serves as a source of short non-coding RNAs (ncRNAs). tRNA-derived ncRNAs show differential expression profiles and play roles in many biological processes beyond translation ...
Honda, Shozo, Kawamura, Takuya, Kirino, PhD, Yohei, Loher, Phillipe, Morichika, Keisuke, Rigoutsos, Isidore   +5 more
core   +2 more sources

Identification of Suitable Reference Genes for qRT-PCR Normalization in Kiwifruit

Horticulturae, 2022
Reference genes are used for the correction of qRT-PCR data, and it is necessary to investigate the optimum reference gene under certain conditions. The expression levels of seven traditional reference genes ACT1, ACT2, GAPDH, 18S rRNA, UBQ, TUB and CYP ...
Yuanjie Zhou, Hui Xia, Xinling Liu, Zhiyi Lin, Yuqi Guo, Honghong Deng, Jin Wang, Lijin Lin, Qunxian Deng, Xiulan Lv, Kunfu Xu, Dong Liang   +11 more
doaj   +1 more source