Results 11 to 20 of about 1,617,447 (322)

Identification and Evaluation of qRT-PCR Reference Genes in Melanaphis sacchari. [PDF]

Insects
Appropriate reference genes must be selected for accurate qRT-PCR data to conduct a thorough gene expression analysis in the sorghum aphid (Melanaphis sacchari, Hemiptera, Aphididae). This approach will establish a foundation for gene expression analysis and determines the efficacy of RNA interference in the sorghum aphid.
Zou K, Wang T, Guan M, Liu Y, Li J, Liu Y, Du J, Wu D.   +7 more
europepmc   +4 more sources

Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR

Genes, 2022
MicroRNAs (miRNAs) are promising molecules that can regulate gene expression, and their expression level and type have been associated with early diagnosis, targeted therapy, and prognosis of various diseases.
E. Ban, E. J. Song
semanticscholar   +1 more source

Development of a multiplex qRT-PCR assay for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine Deltacoronavirus

Frontiers in Veterinary Science, 2023
Currently, porcine coronaviruses are prevalent in pigs, and due to the outbreak of COVID-19, porcine coronaviruses have become a research hotspot.
Yan Li, Jiawei Niu, Xia Zhou, Pinpin Chu, Kun Zhang, Hong-chao Gou, Dong-xia Yang, Jian-Feng Zhang, Chun-ling Li, Ming Liao, S. Zhai   +10 more
semanticscholar   +1 more source

Real-Time qPCR/qRT-PCR Methods: Standard Curves

BioTechniques, 2010
Kristie Nybo
doaj   +2 more sources

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae)

Frontiers in Physiology, 2023
Introduction: Gene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects.
Qingfang Hou, Linlin Yuan, Haifeng Jin, Han Yan, Fen Li, Shao-ying Wu   +5 more
semanticscholar   +1 more source

Reference Genes for Expression Analyses by qRT-PCR in Phthorimaea operculella (Lepidoptera: Gelechiidae)

Insects, 2022
Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is a momentous tool for calculating the expression levels of targeted genes across various experimental conditions.
Chen-Hui Shen, Liyun Peng, Yu-Xing Zhang, Hualiang Zeng, Hong Yu, Lin Jin, Guo-Qing Li   +6 more
semanticscholar   +1 more source

Concentration and Quantification of SARS-CoV-2 RNA in Wastewater Using Polyethylene Glycol-Based Concentration and qRT-PCR

Methods and Protocols, 2021
Wastewater-based epidemiology has become an important tool for the surveillance of SARS-CoV-2 outbreaks. However, the detection of viruses in sewage is challenging and to date there is no standard method available which has been validated for the ...
K. Farkas, Luke S. Hillary, Jamie Thorpe, David I. Walker, J. Lowther, J. McDonald, S. Malham, Davey L. Jones   +7 more
semanticscholar   +1 more source

Evaluation of Optimal Reference Genes for qRT-PCR Analysis in Hyphantria cunea (Drury)

Insects, 2022
Simple Summary Reverse transcription-quantitative polymerase chain reaction (qRT-PCR) has rapidly become the most accessible and widely-applied method for quantifying gene transcription.
Xudong Zhao, Yishu Geng, Tianyi Hu, Yongang Zhao, Suling Yang, D. Hao   +5 more
semanticscholar   +1 more source

A qRT-PCR Method Capable of Quantifying Specific Microorganisms Compared to NGS-Based Metagenome Profiling Data

Microorganisms, 2022
Metagenome profiling research using next-generation sequencing (NGS), a technique widely used to analyze the diversity and composition of microorganisms living in the human body, especially the gastrointestinal tract, has been actively conducted, and ...
Jinuk Jeong, Seyoung Mun, Yunseok Oh, Chun-Sung Cho, Kyeongeui Yun, Yongju Ahn, W. Chung, M. Lim, K. Lee, Tae Soon Hwang, Kyudong Han   +10 more
semanticscholar   +1 more source

Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management

Viruses, 2021
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in Wuhan, China, in late 2019 and is the causative agent of the coronavirus disease 2019 (COVID-19) pandemic.
M. Brandolini, F. Taddei, Maria Michela Marino, L. Grumiro, Agata Scalcione, M. Turba, F. Gentilini, M. Fantini, S. Zannoli, Giorgio Dirani, V. Sambri   +10 more
semanticscholar   +1 more source