Results 21 to 30 of about 1,617,447 (322)

MiR-26a-5p as a Reference to Normalize MicroRNA qRT-PCR Levels in Plasma Exosomes of Pediatric Hematological Malignancies

Cells, 2021
Plasma exosomal microRNAs (miRNAs) are considered as valid circulating biomarkers for cancer diagnosis and prognosis. Quantitative real-time polymerase chain reaction (qRT-PCR), the most commonly used technique to assess circulating miRNA levels ...
C. C. Damanti, Enrico Gaffo, F. Lovisa, A. Garbin, Piero Di Battista, I. Gallingani, A. Tosato, M. Pillon, E. Carraro, M. Mascarin, C. Elia, A. Biffi, S. Bortoluzzi, L. Mussolin   +13 more
semanticscholar   +1 more source

Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR primer-probe sets

medRxiv, 2020
The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions.
C. Vogels, A. Brito, A. Wyllie, J. Fauver, I. Ott, C. Kalinich, M. Petrone, A. Casanovas-Massana, M. C. Muenker, A. Moore, J. Klein, Peiwen Lu, Alice Lu-Culligan, Xiaodong Jiang, Daniel J. Kim, Eriko Kudo, Tianyang Mao, Miyu Moriyama, J. E. Oh, Annsea Park, Julio Silva, E. Song, Takehiro Takehashi, M. Taura, M. Tokuyama, A. Venkataraman, Orr-El Weizman, P. Wong, Yexin Yang, N. R. Cheemarla, Elizabeth B White, S. Lapidus, Rebecca Earnest, B. Geng, P. Vijayakumar, C. Odio, John B. Fournier, S. Bermejo, S. Farhadian, C. D. Dela Cruz, A. Iwasaki, Albert I. Ko, Marie L. Landry, E. Foxman, N. Grubaugh   +44 more
semanticscholar   +1 more source

Selection and Evaluation of Reference Genes for qRT-PCR in Spodoptera frugiperda (Lepidoptera: Noctuidae)

Insects, 2021
Simple Summary The fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is an extremely important omnivorous agricultural pest, it poses a severe threat to food security and agricultural production.
Shipeng Han, Qiuju Qin, Da Wang, Yayuan Zhou, Yunzhuan He   +4 more
semanticscholar   +1 more source

An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5

Veterinary Sciences, 2023
A duplex real-time quantitative reverse transcription-polymerase chain reaction (dqRT-PCR) assay was successfully developed to simultaneously detect canine parainfluenza virus 5 (CPIV5) and a canine endogenous internal positive control (EIPC) in canine ...
Gyu-Tae Jeon, Hye-Ryung Kim, Yeun-Kyung Shin, Oh-Kyu Kwon, Hae-Eun Kang, Oh-Deog Kwon, Choi-Kyu Park   +6 more
doaj   +1 more source

Selection and evaluation of reference genes for qRT-PCR of Scutellaria baicalensis Georgi under different experimental conditions

Molecular Biology Reports, 2021
Scutellaria baicalensis Georgi is a famous medicinal plant with its dried roots having been used as a traditional Chinese medicinal for more than 2000 years.
Wentao Wang, Suying Hu, Yao Cao, R. Chen, Zhe-zhi Wang, Xiaoyan Cao   +5 more
semanticscholar   +1 more source

Development of a one-step multiplex qRT–PCR assay for the detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus

BMC Veterinary Research, 2021
Background African swine fever virus (ASFV), classical swine fever virus (CSFV) and atypical porcine pestivirus (APPV) have caused great economic losses to the swine industry in China. Since coinfections of ASFV, CSFV and APPV occur in certain pig herds,
Huixin Liu, Kaichuang Shi, Jing Zhao, Yanwen Yin, Yating Chen, Hongbin Si, Sujie Qu, Feng Long, Wenjun Lu   +8 more
semanticscholar   +1 more source

Clinical Value of S-poly (T) plus Real-time PCR in Detection of Liver Cancer-related miR-199

Zhongliu Fangzhi Yanjiu, 2020
Objective To explore the advantages and disadvantages of S-poly(T) plus qRT-PCR and Poly (A) qRT-PCR methods in detecting peripheral blood serum miR-199.
WU Juan, ZHANG Yaqin, WU Yunbin, CHEN Min, YU Zhou, CAO Yingping   +5 more
doaj   +1 more source

Spit or Swab? The Diagnostic Accuracy of Saliva-Based Testing as a Sars-Cov-2 Surveillance Tool

Philippine Journal of Pathology, 2022
Background. Nasopharyngeal swab/oropharyngeal swab (NPS/OPS) qRT-PCR is the gold standard for detecting SARS-CoV-2. However, it has its own limitations including cost and invasiveness.
Raymundo Lo, Farrah Kristine Santiago, Grig Misiona, Melani Sionzon, Danielle Anne Gonong, Jill Jaime, Jan Roman Ayco, Aquiles Joseph Lira, Erik Francis Rarugal, Jon Carlo Crisostomo, Manuel Bautista Jr., Kenneth Chrysostom Ibarra, Eunice Jane Ciriaco, Arielle Trish Zamora, Kimberly Abundo, Emmylou Tarnate, Jonathan Nario, Jan Joseph Hernandez, Kathleen Mae Montes, Ma. Anabella Recio   +19 more
doaj   +1 more source

Preconditioning of mesenchymal stromal cells with low-intensity ultrasound: influence on chondrogenesis and directed SOX9 signaling pathways [PDF]

, 2020
Background: Continuous low-intensity ultrasound (cLIUS) facilitates the chondrogenic differentiation of human mesenchymal stromal cells (MSCs) in the absence of exogenously added transforming growth factor-beta (TGFβ) by upregulating the expression of ...
A Argentaro, A Augello, A Katsumi, A Woods, A Woods, AD Miller, AH Huang, B Yang, BH Choi, C Huang, C Matta, CA Simmons, CB Little, CJ O’Conor, CY Huang, D Schumann, DR Haudenschild, EM Darling, F Dell'Accio, G Coricor, H Sim, H-C Hsu, HJ Kwon, HJ Lee, HJ Lee, J Li, J Puetzer, JC Sng, JD Kisiday, JH Cui, JH Cui, JW Choi, K Ebisawa, K Pelttari, L Bian, Li Ling, LJ Ng, M Shakibaei, M Tsuda, MA Corrigan, MB Mueller, N Fahy, N Thelin, NP Whitney, P Müller, R Ogawa, RL Mauck, RM Delaine-Smith, RS Tigli, S Murakami, S Noriega, S Noriega, S Yamaguchi, SD Cook, SD Cook, T Furumatsu, T Guo, T Ito, T Juhasz, T Nishida, T Sanjukta Guha, TM Louw, V Lefebvre, V Lefebvre, W Huang, W Huang, Z Li, ZJ Zhang   +67 more
core   +2 more sources

Development of a multiplex qRT-PCR assay for detection of African swine fever virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus

Journal of Veterinary Sciences, 2021
Background African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China.
Yating Chen, Kaichuang Shi, Huixin Liu, Yanwen Yin, Jing Zhao, Feng Long, Wenjun Lu, Hongbin Si   +7 more
semanticscholar   +1 more source