Results 31 to 40 of about 1,617,447 (322)

Selection and validation reference genes for qRT-PCR normalization in different cultivars during fruit ripening and softening of peach (Prunus persica)

Scientific Reports, 2021
Quantitative real-time PCR (qRT-PCR) has been emerged as an effective method to explore the gene function and regulatory mechanisms. However, selecting appropriate reference gene (s) is a prerequisite for obtaining accurate qRT-PCR results.
Shuanghong You, K. Cao, Changwen Chen, Yong Li, Jinlong Wu, G. Zhu, W. Fang, Xin-Wei Wang, Lirong Wang   +8 more
semanticscholar   +1 more source

Keeping qRT-PCR rigorous and biologically relevant [PDF]

Plant Cell Reports, 2014
Because of the importance of differential gene expression in the growth, development, and reproduction of organisms, assays of mRNA transcript levels are widely employed in biological research. The most frequently used assay combines reverse transcription (RT) with quantitative polymerase chain reaction (qPCR) and is known as qRT-PCR (or RT-qPCR).
John, Bennett, David, Hondred, James C, Register   +2 more
openaire   +2 more sources

Selection and Validation of Reference Genes For qRT-PCR Analysis of Rhopalosiphum padi (Hemiptera: Aphididae)

Frontiers in Physiology, 2021
Rhopalosiphum padi (L.) (Hemiptera: Aphididae) is an important cosmopolitan pest in cereal crops. Reference genes can significantly affect qRT-PCR results. Therefore, selecting appropriate reference genes is a key prerequisite for qRT-PCR analyses.
Mengyi Li, Xinan Li, Chao Wang, Qiuchi Li, Saige Zhu, Yunhui Zhang, Xiangrui Li, Fengshan Yang, Xun Zhu   +8 more
semanticscholar   +1 more source

qRT-PCR: a method and its difficulties [PDF]

Naunyn-Schmiedeberg's Archives of Pharmacology, 2012
Today, quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the favored method to quantify gene expression in molecular biology and clinical studies. qRTPCR analyses can be performed in a high-throughput manner and are very sensitive and specific, cost-effective, and reproducible.
Franziska, Bollmann, Ingrid, Casper, Jenny, Henke, Andrea, Pautz   +3 more
openaire   +2 more sources

Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra, Chandrashekhar Raut, Neelam Prasad, J S Bhawalkar, Safia Farooqui, Shahzad Mirza, Veshal V Madan   +6 more
doaj   +1 more source

Development of reverse-transcription PCR techniques to analyse the density and sex ratio of gametocytes in genetically diverse Plasmodium chabaudi infections [PDF]

, 2007
We have developed cross-genotype and genotype-specific quantitative reverse-transcription PCR (qRT-PCR) assays to detect and quantify the number of parasites, transmission stages (gametocytes) and male gametocytes in blood stage Plasmodium chabaudi ...
Abdel-Wahab, Alano, Babiker, Bell, Cheeseman, Damien R. Drew, De Roode, De Roode, De Roode, Dye, Ferguson, Ferguson, Foster, Gardner, Gautret, Hamilton, Khan, Mackinnon, Nee, Paterson, Paul, Paul, Paul, Pinheiro, Ranford-Cartwright, Read, Read, Read, Reece, Reece, Reece, Sarah E. Reece, Schall, Schall, Schneider, Shutler, Sinden, Taylor, Taylor, Wargo, Wargo, West, West, West, West   +44 more
core   +1 more source

FUS mutant human motoneurons display altered transcriptome and microRNA pathways with implications for ALS pathogenesis [PDF]

, 2017
The FUS gene has been linked to amyotrophic lateral sclerosis (ALS). FUS is a ubiquitous RNA-binding protein, and the mechanisms leading to selective motoneuron loss downstream of ALS-linked mutations are largely unknown.
Alfano, Vincenzo, Bozzoni, Irene, Colantoni, Alessio, DE SANTIS, Riccardo, DE TURRIS, Valeria, Peruzzi, Giovanna, Rosa, Alessandro, Santini, Laura   +7 more
core   +5 more sources

Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

BMC Biotechnology, 2021
Background Although quantitative real-time PCR (qRT-PCR) is a common and sensitive method for miRNAs analysis, it is necessary to optimize conditions and minimize qRT-PCR inhibitors to achieve reliable results.
E. Ban, Haejin Kwon, H. Seo, Y. Yoo, E. J. Song   +4 more
semanticscholar   +1 more source

Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Virology Journal, 2020
Background Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for clinical ...
Yang Zhang, Chunyang Dai, Huiyan Wang, Yong Gao, Tuantuan Li, Yan Fang, Zuojun Shen, Lichang Chen, Zhaowu Chen, Xuejun Ma, Ming Li   +10 more
doaj   +1 more source

A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test [PDF]

, 2014
A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species.
De Craeye, Stéphane, Francart, Aurélie, Kalai, Michael, Lamoral, Sophie, Nazé, Florence, Suin, Vanessa, Van Gucht, Steven   +6 more
core   +4 more sources