Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus
Veterinary Sciences, 2023 Atypical porcine pestivirus (APPV), a newly discovered virus, is associated with the type A-II congenital tremor (CT) in neonatal piglets. APPV distributes throughout the world and causes certain economic losses to the swine industry.
Huixin Liu +5 more
doaj +1 more source
SARS-CoV-2 genomic diversity and the implications for qRT-PCR diagnostics and transmission
Genome Research, 2021 The COVID-19 pandemic has sparked an urgent need to uncover the underlying biology of this devastating disease. Though RNA viruses mutate more rapidly than DNA viruses, there are a relatively small number of single nucleotide polymorphisms (SNPs) that ...
Nicolae Sapoval +19 more
semanticscholar +1 more source
Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens. [PDF]
, 2015 BackgroundTo determine differentially expressed and spliced RNA transcripts in chronic lymphocytic leukemia specimens a high throughput RNA-sequencing (HTS RNA-seq) analysis was performed.MethodsTen CLL specimens and five normal peripheral blood CD19+ B ...
A Ameur +72 more
core +1 more source
Scientific Reports, 2021 To meet the unprecedented requirement of diagnostic testing for SARS-CoV-2, a large number of diagnostic kits were authorized by concerned authorities for diagnostic use within a short period of time during the initial phases of the ongoing pandemic.
J. Singh +12 more
doaj +1 more source
Acta Biológica Colombiana, 2015 Las raíces almacenadoras de yuca representan una fuente importante de almidón. La ruta metabólica del almidón ha sido reconstruida recientemente en yuca gracias a la liberación de la secuencia completa de su genoma. En este estudio se evaluó la expresión
Simón Pedro Cortés Sierra +3 more
doaj +1 more source
Human naive CD8 T cells down-regulate expression of the WNT pathway transcription factors lymphoid enhancer binding factor 1 and transcription factor 7 (T cell factor-1) following antigen encounter in vitro and in vivo [PDF]
, 2006 The transcription factors lymphoid enhancer binding factor 1 (LEF1) and transcription factor 7 (TCF7) (T cell factor-1 (TCF-1)) are downstream effectors of the WNT signaling pathway, which is a critical regulator of T cell development in the thymus.
Callan, Margaret F C +5 more
core +1 more source
Blocking CD248 molecules in perivascular stromal cells of patients with systemic sclerosis strongly inhibits their differentiation toward myofibroblasts and proliferation: A new potential target for antifibrotic therapy [PDF]
, 2018 Background: Fibrosis may be considered the hallmark of systemic sclerosis (SSc), the end stage triggered by different pathological events. Transforming growth factor-β (TGF-β) and platelet-derived growth factor BB (PDGF-BB) are profibrotic molecules ...
Berardicurti O. +11 more
core +1 more source
Covid-19 Detection using qRT-PCR - A Review
Journal for Research in Applied Sciences and Biotechnology, 2022 In the ongoing Covid pandemic, the monitoring of SARSnCoV-2 with the help of viral loads/viral kinetics has become more essential via the RT-PCR technique. However, the interpretations of qRT-PCR technique results are made as qualitative and quantitative or semi-quantitative, and day by day, this interpretation is becoming more important.
Yagyavalkya Sharma +4 more
openaire +1 more source
Emerging Infectious Diseases, 2013 We compared rotavirus detection rates in children with acute gastroenteritis (AGE) and in healthy controls using enzyme immunoassays (EIAs) and semiquantitative real-time reverse transcription PCR (qRT-PCR).
Jacqueline E. Tate +14 more
doaj +1 more source
Detection of ROS1 gene rearrangement in lung adenocarcinoma: comparison of IHC, FISH and real-time RT-PCR. [PDF]
PLoS ONE, 2015 AIMS:To compare fluorescence in situ hybridization (FISH), immunohistochemistry (IHC) and quantitative real-time reverse transcription-PCR (qRT-PCR) assays for detection of ROS1 fusion in a large number of ROS1-positive lung adenocatcinoma (ADC) patients.
Ling Shan +6 more
doaj +1 more source