Results 51 to 60 of about 1,617,447 (322)

Selection and validation of reference genes for qRT‐PCR analysis during fruit ripening of red pitaya (Hylocereus polyrhizus)

open access: yesFEBS Open Bio, 2021
Red pitaya (Hylocereus polyrhizus) is widely cultivated in southern and southwestern China. To provide a basis for studying the molecular mechanisms of the ripening of this fruit, we carried out RNA sequencing (RNA‐seq) analysis to identify ...
Qianming Zheng   +3 more
doaj   +1 more source

ER and HER2 expression are positively correlated in HER2 non-overexpressing breast cancer [PDF]

open access: yes, 2012
PMCID: PMC3446380This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided ...
A Borg   +48 more
core   +5 more sources

Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases

open access: yesJournal of Translational Medicine, 2017
Background Immunohistochemistry (IHC) and fluorescent-in situ hybridization (FISH) are standard methods to assess human epidermal growth factor receptor 2 (HER2) status in breast cancer (BC) patients. Real-time quantitative polymerase-chain-reaction (qRT-
Gabriele Zoppoli   +16 more
doaj   +1 more source

Genes for asparagine metabolism in Lotus japonicus : differential expression and interconnection with photorespiration [PDF]

open access: yes, 2017
Background: Asparagine is a very important nitrogen transport and storage compound in plants due to its high nitrogen/carbon ratio and stability.
Betti, Marco   +5 more
core   +1 more source

Serum microRNA array analysis identifies miR-140-3p, miR-33b-3p and miR-671-3p as potential osteoarthritis biomarkers involved in metabolic processes. [PDF]

open access: yes, 2017
Background: MicroRNAs (miRNAs) in circulation have emerged as promising biomarkers. In this study, we aimed to identify a circulating miRNA signature for osteoarthritis (OA) patients and in combination with bioinformatics analysis to evaluate the utility
A Brazma   +59 more
core   +2 more sources

Development of a panel of three multiplex allele-specific qRT-PCR assays for quick differentiation of recombinant variants and Omicron subvariants of SARS-CoV-2

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Quick differentiation of the circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmission.
Jianguo Li   +12 more
doaj   +1 more source

The biogenesis pathway of tRNA-derived piRNAs in Bombyx germ cells. [PDF]

open access: yes, 2017
Transfer RNAs (tRNAs) function in translational machinery and further serves as a source of short non-coding RNAs (ncRNAs). tRNA-derived ncRNAs show differential expression profiles and play roles in many biological processes beyond translation ...
Honda, Shozo   +5 more
core   +2 more sources

Evaluation of bacterial internalization using qRT-PCR [PDF]

open access: yesGERMS, 2014
Bacterial resistance to antibiotics currently represents a topic of high interest. Unfortunately, studies addressing their adaptation and multidrug tolerance latency1 are not sufficient for a comprehensive description of the pathogen-host molecular interaction mechanisms (interactome) and the bacterial internalization into human cells, which leads to ...
openaire   +2 more sources

RNA isolation and qRT-PCR v1

open access: yes, 2023
This protocol describes the RNA isolation from cultured cells and quantitative RT PCR.
Narayana Yadavalli, Shawn M. Ferguson
openaire   +1 more source

Systemic suppression of the shoot metabolism upon rice root nematode infection [PDF]

open access: yes, 2014
Hirschmanniella oryzae is the most common plant-parasitic nematode in flooded rice cultivation systems. These migratory animals penetrate the plant roots and feed on the root cells, creating large cavities, extensive root necrosis and rotting.
Bauters, Lander   +4 more
core   +14 more sources

Home - About - Disclaimer - Privacy