Results 61 to 70 of about 1,617,447 (322)

Statistical modelling of transcript profiles of differentially regulated genes [PDF]

, 2008
Background: The vast quantities of gene expression profiling data produced in microarray studies, and the more precise quantitative PCR, are often not statistically analysed to their full potential.
Burton, Kerry S., Eastwood, Daniel C., Mead, A. (Andrew), Sergeant, Martin J.   +3 more
core   +4 more sources

Assessment of mRNA Splice Variants by qRT-PCR [PDF]

, 2013
Alternative splicing is an essential process for the generation of protein diversity. The physiological role, cellular localization, and abundance of splice variant products compared to the wild-type protein may be completely different. This is illustrated by the five splice variants of the antiapoptotic protein survivin that are more abundant in ...
Ileabett M Echevarria, Vargas, Pablo E, Vivas-Mejía   +1 more
openaire   +2 more sources

Regulation of sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 and their epigenetic status in medulloblastoma and astrocytoma [PDF]

, 2010
Background The Sonic hedgehog (Shh) signaling pathway is critical for cell growth and differentiation. Impairment of this pathway can result in both birth defects and cancer.
Afzal, Mohammad, Castresana, Javier S, Eberhart, Charles G., Fan, Xing, Rey, Juan A, Shahi, Mehdi H, Sinha, Subrata   +6 more
core   +5 more sources

Reference gene selection for qRT-PCR analysis of season- and tissue-specific gene expression profiles in the honey bee Apis mellifera

Scientific Reports, 2020
Honey bees are both important pollinators and model insects due to their highly developed sociality and colony management. To better understand the molecular mechanisms underlying honey bee colony management, it is important to investigate the expression
J. Jeon, Kyung-Hwan Moon, YeongHo Kim, Young Ho Kim   +3 more
semanticscholar   +1 more source

M gene targeted qRT-PCR approach for SARS-CoV-2 virus detection

Scientific Reports, 2023
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is the gold standard method for SARS-CoV-2 detection, and several qRT-PCR kits have been established targeting different genes of the virus.
Md. Murshed Hasan Sarkar, Showti Raheel Naser, Sanjana Fatema Chowdhury, Md. Salim Khan, Md. Ahashan Habib, Shahina Akter, Tanjina Akhtar Banu, Barna Goswami, Iffat Jahan, Maksudur Rahman Nayem, Md. Akibul Hassan, Md. Imran Khan, Mohammad Fazle Alam Rabbi, Chowdhury Rafiqul Ahsan, Md. Ibrahim Miah, Afzalun Nessa, S. M. Rashed Ul Islam, Mohammed Atiqur Rahman, Md. Aftab Ali Shaikh, Md. Sharfuddin Ahmed   +19 more
doaj   +1 more source

Experimental assessment of robust reference genes for qRT-PCR in lung cancer studies

Frontiers in Oncology, 2023
Stable internal reference genes are crucial for quantitative real-time PCR (qRT-PCR) analyses in lung cancer studies. Widely used reference genes are mostly chosen by intuition or from pan-cancer transcriptome data and lack experimental validation by qRT-
Wei Gu, Wei Gu, Yubin Wang, Ran Xu, Jiamin Li, Jingjie Jin, Jing Zhao, Yang Chen, Yuanzhi Lu, Gong Zhang   +9 more
doaj   +1 more source

MiR-33a Controls hMSCS Osteoblast Commitment Modulating the Yap/Taz Expression Through EGFR Signaling Regulation [PDF]

, 2019
Mesenchymal stromal cells (hMSCs) display a pleiotropic function in bone regeneration. The signaling involved in osteoblast commitment is still not completely understood, and that determines the failure of current therapies being used.
Alessandro, Riccardo, Bellavia, Daniele, Carina, Valeria, Conigliaro, Alice, Costa, Viviana, De Luca, Angela, Fini, Milena, Giavaresi, Gianluca, Raimondi, Lavinia, Salamanna, Francesca   +9 more
core   +1 more source

Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs

bioRxiv, 2020
The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19 which has become a global concern due to its rapid spread. Meanwhile, increased demand in testing has led to shortage of reagents, supplies, and compromised
T. Toptan, S. Hoehl, S. Westhaus, D. Bojkova, A. Berger, Björn Rotter, K. Hoffmeier, S. Ciesek, M. Widera   +8 more
semanticscholar   +1 more source

Evaluation of the performance of a multiplex reverse transcription polymerase chain reaction kit as a potential diagnostic and surveillance kit for rotavirus in Kenya

Tropical Diseases, Travel Medicine and Vaccines, 2019
Background Diarrhea is a serious concern worldwide, especially in developing countries. Rotavirus is implicated in approximately 400,000 infant deaths annually.
Cliff Odhiambo Philip, Margaret Koech, Nancy Kipkemoi, Ronald Kirera, Janet Ndonye, Abigael Ombogo, Mary Kirui, Erick Kipkirui, Brook Danboise, Christine Hulseberg, Stacey Bateman, Alexander Flynn, Brett Swierczewski, Esther Magiri, Elizabeth Odundo   +14 more
doaj   +1 more source

Single cell high-throughput qRT-PCR protocol

, 2021
Abstract Single cell high-throughput qRT-PCR protocol combines high sensitivity technique of single cell qPCR with high-throughput qPCR technology that can generate data from 96 samples and 96 genes in a single experiment. It can be adapted for various sample types- cell culture, tissue samples and extracted RNA (10 pg) and measured on ...
Sirisha Achanta, Rajanikanth Vadigepalli
openaire   +1 more source