Results 71 to 80 of about 1,617,447 (322)

Development of a qRT - PCR Diagnostic Kit for the Detection of FCoV

Cyprus Journal of Medical Sciences
BACKGROUND/AIMS: In recent years, feline infectious peritonitis (FIP), which led to the death of more than 3,000 cats on the island of Cyprus, has become a significant concern. FIP is a fatal disease in cats caused by feline coronavirus (FCoV).
Gülten Tuncel Dereboylu, Gökçe Akan, Melis Kalaycı, Cenk Serhan Özverel, Tamer Şanlıdağ   +4 more
doaj   +1 more source

Putative Internal Control Genes in Bovine Milk Small Extracellular Vesicles Suitable for Normalization in Quantitative Real Time-Polymerase Chain Reaction

Membranes, 2021
Bovine milk small extracellular vesicles (sEVs) contain many biologically important molecules, including mRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used method for quantifying mRNA in tissues and cells. However, the use,
Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima   +6 more
doaj   +1 more source

RNA interference in marine and freshwater sponges [PDF]

, 2011
Background: The marine sponge Tethya wilhelma and the freshwater sponge Ephydatia muelleri are emerging model organisms to study evolution, gene regulation, development, and physiology in non-bilaterian animal systems. Thus far, functional methods (i.e.,
Cieniewicz, Brandon, Danka, Elizabeth S., Haen, Karri M., Hammel, Jörg U., Hill, April L., Hill, Malcolm S., Knight, Scott W., Lavrov, Dennis V., Nickel, Michael, Posfai, Dora, Rivera, Ajna S., Winters, Ian P., Wörheide, Gert   +12 more
core   +1 more source

Transcription analysis of apple fruit development using cDNA microarrays [PDF]

, 2009
The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding.
Costa, F., Gianfranceschi, L., Molthoff, J.W., Schouten, H.J., Soglio, V., Weemen-Hendriks, M.   +5 more
core   +5 more sources

Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay

Diagnostic Pathology, 2017
Background Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where
Xiaodong Lyu, Xianwei Wang, Lina Zhang, Zhenzhu Chen, Yu Zhao, Jieying Hu, Ruihua Fan, Yongping Song   +7 more
doaj   +1 more source

One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples [PDF]

PeerJ, 2016
Background. Group A rotavirus (RVA) infection is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live-attenuated rotavirus vaccines, RotaTeq® and Rotarix®, has dramatically reduced RVA associated AGE and ...
Rashi Gautam, Slavica Mijatovic-Rustempasic, Mathew D. Esona, Ka Ian Tam, Osbourne Quaye, Michael D. Bowen   +5 more
doaj   +2 more sources

Cannabidiol stimulates AML-1a-dependent glial differentiation and inhibits glioma stem-like cells proliferation by inducing autophagy in a TRPV2-dependent manner [PDF]

, 2015
Glioma stem-like cells (GSCs) correspond to a tumor cell subpopulation, involved in glioblastoma multiforme (GBM) tumor ini- tiation and acquired chemoresistance.
Amantini, Consuelo, Liberati, Sonia, Morelli, MARIA BEATRICE, Nabissi, Massimo, Pallini, Roberto, Ricci Vitiani, Lucia, Santoni, Giorgio, Santoni, Matteo   +7 more
core   +2 more sources

Identification of a Proliferation Gene Cluster Associated with HPV E6/E7 Expression Level and Viral DNA Load in Invasive Cervical Carcinoma

, 2005
Specific HPV DNA sequences are associated with more than 90% of invasive carcinomas of the uterine cervix. Viral E6 and E7 oncogenes are key mediators in cell transformation by disrupting TP53 and RB pathways. To investigate molecular mechanisms involved
Anne de La Rochefordière, Christophe Rosty, Dafna Tsafrir, Eytan Domany, F Ferlay, François Radvanyi, Ilan Tsafrir, Jean Paul Thiery, Jérôme Couturier, Martine Peter, Michal Sheffer, Nicolas Stransky, Patricia de Crémoux, Rémy Salmon, Thierry Dorval, Xavier Sastre-Garau   +15 more
core   +1 more source

microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma. [PDF]

, 2017
Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (HIF-1α), a gene known to promote ...
Cao, Ke, Chen, Jia, Chen, Xiang, Chen, Yao, Chen, Yong, Cheng, Yan, Li, Jingjing, Qian, Li, Tang, Jintian, Tang, Shijie, Wang, Aijun, Xiong, Wei, Zhou, Jianda   +12 more
core   +1 more source

qRT-PCR sample preparation v1

, 2023
Protocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
openaire   +1 more source