Results 81 to 90 of about 1,617,447 (322)

The RNA-binding protein HuR contributes to neuroinflammation by promoting C-C chemokine receptor 6 (CCR6) expression on Th17 cells. [PDF]

, 2017
In both multiple sclerosis and experimental autoimmune encephalomyelitis (EAE), the C-C chemokine receptor 6 (CCR6) is critical for pathogenic T helper 17 (Th17) cell migration to the central nervous system (CNS).
Atasoy, Ulus, Chen, Jing, Cramer, Carole, Drew, Paul D., Gorospe, Myriam, Martindale, Jennifer L., Yu, Shiguang   +6 more
core   +2 more sources

Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions

Scientific Reports, 2020
Selecting suitable internal reference genes is an important prerequisite for the application of quantitative real-time PCR (qRT-PCR). However, no systematic studies have been conducted on reference genes in luffa.
mindong chen, Bin Wang, Yongping Li, Mei-juan Zeng, Jianting Liu, Xin-ru Ye, Hailong Zhu, Qingfang Wen   +7 more
semanticscholar   +1 more source

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Standardization of Gene Expression Quantification by Absolute Real-Time qRT-PCR System Using a Single Standard for Marker and Reference Genes

Biomarker Insights, 2010
In the last decade, genome-wide gene expression data has been collected from a large number of cancer specimens. In many studies utilizing either microarray-based or knowledge-based gene expression profiling, both the validation of candidate genes and ...
Yi-Hong Zhou, Vinay R. Raj, Eric Siegel, Liping Yu   +3 more
doaj   +2 more sources

A quantitative real time PCR method to analyze T cell receptor Vβ subgroup expansion by staphylococcal superantigens

Journal of Translational Medicine, 2010
Background Staphylococcal enterotoxins (SEs), SE-like (SEl) toxins, and toxic shock syndrome toxin-1 (TSST-1), produced by Staphylococcus aureus, belong to the subgroup of microbial superantigens (SAgs).
Terman David S, Park Joo Youn, Seo Keun Seok, Bohach Gregory A   +3 more
doaj   +1 more source

Transcriptome profiling reveals significant changes in the gastric muscularis externa with obesity that partially overlap those that occur with idiopathic gastroparesis [PDF]

, 2019
BACKGROUND: Gastric emptying is impaired in patients with gastroparesis whereas it is either unchanged or accelerated in obese individuals. The goal of the current study was to identify changes in gene expression in the stomach muscularis that may be ...
Chen, Meng, Choi, Jennifer N., Gupta, Anita, Herring, B. Paul, Hoggatt, April M., Mihaylov, Plamen, Nakeeb, Attila, Wo, John M.   +7 more
core   +1 more source

Rapid, sensitive and specific SARS coronavirus-2 detection: a multi-center comparison between standard qRT-PCR and CRISPR based DETECTR

medRxiv, 2020
Recent advances in CRISPR-based diagnostics suggest that DETECTR, a combination of isothermal reverse transcriptase loop mediated amplification (RT-LAMP) and subsequent Cas12 bystander nuclease activation by amplicon targeting ribonucleoprotein complexes,
E. Brandsma, H. Verhagen, T. J. van de Laar, E. Claas, M. Cornelissen, Emile van den Akker   +5 more
semanticscholar   +1 more source

PARP inhibitors elicit distinct transcriptional programs in homologous recombination competent castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
PARP inhibitors are used to treat a small subset of prostate cancer patients. These studies reveal that PARP1 activity and expression are different between European American and African American prostate cancer tissue samples. Additionally, different PARP inhibitors cause unique and overlapping transcriptional changes, notably, p53 pathway upregulation.
Moriah L. Cunningham, Jasibel Vasquez‐Gonzalez, Samantha M. Barnada, Salome Tchotorlishvili, Latese Jones, Ryan Maguire, Genevieve Lewis, Kinza Rizwan, Jenny Deng, Salma Koachar, Drithi Patel, Hailey Shankle, Tessa Mulders, Namra Ajmal, Charalambos Solomides, Emad S. Alnemri, Teresa F. Alnemri, Ayesha A. Shafi, Leonard G. Gomella, Wm Kevin Kelly, Steven B. McMahon, Matthew J. Schiewer   +21 more
wiley   +1 more source

qRT-PCR v1

, 2017
qRT- PCR for each gene was carried out using a thermal cycler (Bio-Rad, Hercules, CA, USA) and amplification conditions were 40 cycles of 30s at 95°C, 3 s at 95°C, and 30 s at 60°C.
openaire   +2 more sources

Characterization of Arabidopsis thaliana CPR5 via the elucidation of interacting protein partners : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Biochemistry at Massey University, Palmerston North, New Zealand [PDF]

, 2015
he Arabidopsis thaliana Constitutive expresser of pathogenesis related genes5 (CPR5) has previously been suggested to play a role in the regulation of disease resistance, plant and cell proliferation, development and death.
Chiem, Fiona (Shane)
core