Results 71 to 80 of about 327,904 (307)

Determination of Chitin Content in Insects: An Alternate Method Based on Calcofluor Staining

Frontiers in Physiology, 2020
Chitin is an aminopolysaccharide present in yeast cells and arthropod cuticle and is one of the most abundant biopolymers. The conventional methods for the quantitation of chitin content in biological samples are based on its hydrolysis (acid or ...
Bianca Santos Henriques, Eloi Souza Garcia, Eloi Souza Garcia, Patricia Azambuja, Fernando Ariel Genta, Fernando Ariel Genta   +5 more
doaj   +1 more source

Strategies for quantitation of phosphoproteomic data

, 2010
Recent developments in phosphoproteomic sample-preparation techniques and sensitive mass spectrometry instrumentation have led to large-scale identifications of phosphoproteins and phosphorylation sites from highly complex samples.
Giuseppe Palmisano, Palmisano, Giuseppe, Tine E Thingholm, Thingholm, Tine Engberg   +3 more
core   +1 more source

Biofilm‐Binding Phages Enhance Biofilm Eradication by Synergistic Photothermal and Photodynamic Therapy

Advanced Science, EarlyView.
Engineered phage‐based trifunctional nanofibers are developed for synergistic biofilm eradication. Biofilm‐binding phages, armed with AuNPs and TCPP, serve as both warheads and scaffolds, endowing the phototherapy agents with biofilm‐anchoring ability. Upon light irradiation, AuNPs induce photothermal therapy, while TCPP generates 1O2 for photodynamic ...
Ying Cao, Tao Yang, Rui Wang, Hui‐Da Li, Xiao‐Yu Zhang, Feng Cheng, Ying Liu, Jian‐Hua Wang, Ting Yang, Chuanbin Mao   +9 more
wiley   +1 more source

Quantitation of Rubisco activase by single radial immunodiffusion

浙江大学学报. 农业与生命科学版, 2001
The antibody against Rubisco activase was produced with rabbit after purification of the enzyme and the technology of single radial immunodiffusion for the enzyme was set up.
JIANG De-An, WENG Xiao-Yan, LU Qing
doaj   +1 more source

Organic Acid Quantitation by NeuCode Methylamidation

, 2016
We have developed a multiplexed quantitative analysis method for carboxylic acids by liquid chromatography high resolution mass spectrometry. The method employs neutron encoded (NeuCode) methylamine labels (13C or 15N enriched) that are affixed to ...
Michael S. Westphall (1314177), Arne Ulbrich (1361829), Joshua J. Coon (388014), Derek J. Bailey (1310010)   +3 more
core   +1 more source

Early Retinal UCHL1 Dysregulation Coupled With Synaptic Loss Reflects Alzheimer's Disease Severity

Advanced Science, EarlyView.
This study identifies synapse‐enriched deubiquitinase UCHL1 as an early Aβ‐responsive regulator of retinal synaptopathy in Alzheimer's disease. Retinal UCHL1 loss accompanies excitatory synapse degeneration, p75NTR activation, and neuroinflammation, and predicts Braak stage and cognitive decline. Aβ42 fibrils trigger synaptic and UCHL1 depletion before
Altan Rentsendorj, Jean‐Philippe Vit, Alexandre Hutton, Yosef Koronyo, Saba Shahin, Edward Robinson, Ernesto Barron, Anthony Rodriguez, Ousman Jallow, Julia Sheyn, Bhakta Prasad Gaire, Lalita Subedi, Miyah R. Davis, Barathan Gnanabharathi, Alexander V. Ljubimov, Stuart L. Graham, Vivek K. Gupta, Mehdi Mirzaei, Debra Hawes, Katlin Silm, Keith L. Black, Lon S. Schneider, Alfredo A. Sadun, Jesse G. Meyer, Dieu‐Trang Fuchs, Maya Koronyo‐Hamaoui   +25 more
wiley   +1 more source

Accurate quantitation of standard peptides used for quantitative proteomics [PDF]

, 2009
MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide ...
Nadia I. Georgieva, Collins, Leonard B., David G. Klapper, Klapper, David G., Tyra J. Cross, James A. Swenberg, Borchers, Christoph H., Swenberg, James A., Narisa K. Bordeerat, Georgieva, Nadia I., Boysen, Gunnar, Gunnar Boysen, Leonard B. Collins, Cross, Tyra J., Bordeerat, Narisa K., Christoph H. Borchers   +15 more
core   +1 more source

Intrinsic Dual‐Phase Regulated GeSe2 Nanoparticles Triggered by Ball‐Milling Treatment for Photonic Multi‐Valued Logic Circuits

Advanced Science, EarlyView.
We report the solid‐state ball milling, a traditional, reliable, mass‐productive material processing, to prepare the air‐stable and dual‐phase GeSe2‐x nanoparticles with extended photodetection feasibility toward optical‐wavelength regions. We further display photonic multi‐valued logic (MVL) circuit through the employment of a hybrid PMMA/GeSe2‐x ...
An‐Ting Tsai, Chun‐Jen Wang, Pin‐Chao Liao, Chin Shan Lue, Chia‐Nung Kuo, Le Vo Phuong Thuan, Po‐Hsuan Hsiao, Chang‐Hyun Kim, Chia‐Yun Chen   +8 more
wiley   +1 more source

FORENSIC SCIENCES Development of a Human DNA Quantitation System [PDF]

, 2001
The AluQuant™ Human DNA Quantitation System has been developed for human-specific quantitation of forensic samples. This system uses probes specific to repetitive genetic elements allowing quantitation without target amplification.
Allan Tereba, Michelle N Mandrekar, Benjamin E Krenke, Aileen M Erickson, Nicole Westphal, Karla Kopp, Rhonda Nelson, Keyna Peterson, John Shultz, Paraj V Mandrekar   +9 more
core  

A Pancreatitis‐Inspired Trypsinogen Nanoplatform Reprograms Tumor‐Associated Macrophages via NF‐κB for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
Trypsinogen‐loaded nanoparticles reprogram TAMs via NF‐κB/NLRP3, driving M2→M1 conversion and potent phagocytosis to unleash antitumor immunity. ABSTRACT Although reprogramming tumor‐associated macrophages (TAMs) represents a promising therapeutic strategy, approaches that are both precise and safe remain scarce.
Lei Cao, Yu He, Wenhao Li, Bo Gong, Han Lin, Jianlin Shi, Yuan Yong, Wencheng Wu   +7 more
wiley   +1 more source