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Quantitative real time PCR for distinction between Pneumocystis jirovecii infection/colonization in hospitalized patients [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundIdentification of the opportunistic fungus Pneumocystis jirovecii in respiratory specimens presents challenges, particularly in differentiating between colonization and active infection.
Faezeh Rouhi, Mahzad Erami, Sepide Rastgufar, Maryam Jahani, Shima Aboutalebian, Shima Aboutalebian, Sajedeh Soltani, Hamed Fakhim, Hossein Mirhendi, Hossein Mirhendi   +9 more
doaj   +2 more sources

Quantitative analysis of the dystrophin gene by real-time PCR [PDF]

Archives of Biological Sciences, 2012
Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Maksimovic Nela, Anđelkovic Ana, Milic-Rasic Vedrana, Rakocevic-Stojanovic Vidosava, Kastratovic-Kotlica Biljana, Brankovic S., Damnjanovic Tatjana, Jekic Biljana, Bunjevacki Vera, Lukovic Ljiljana, Perovic Dijana, Cvjeticanin Suzana, Novakovic Ivana   +12 more
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Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose

Fermentation, 2022
Rummeliibacillus sp. TYF-LIM-RU47, isolated from the fermentation substrate of grain vinegar, could produce acetoin using a variety of carbon sources, including pentose, hexose and lignocellulose.
Guoyang Feng, Xiaojun Fan, Yanna Liang, Chen Li, Junde Xing, Yongji He   +5 more
doaj   +1 more source

Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR

Biomarker Insights, 2012
1p/19q (1p and/or 19q) deletions are prognostic factors in oligodendroglial tumors (OT) and predict better survival after both chemotherapy and radiotherapy. While studying 1p/19q status as a potential variable within multivariate prognosis models for OT,
Abhishek Chaturbedi, Liping Yu, Mark E Linskey, Yi-hong Zhou   +3 more
doaj   +2 more sources

Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

Molecular Plant-Microbe Interactions, 2003
Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf, Katja Manthey, Jasmin Doll, Andreas M. Perlick, Burkhard Linke, Thomas Bekel, Folker Meyer, Philipp Franken, Helge Küster, Franziska Krajinski   +9 more
doaj   +1 more source

Real-Time PCR Assay for Quantitative Mismatch Detection

BioTechniques, 2003
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively, L. Chang, J.M. LeBon, Q. Liu, A.D. Riggs, J. Singer-Sam   +5 more
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Differential gene expression in response to cold stress in Lepidium apetalum during seedling emergence

Biologia Plantarum, 2012
Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived
H. X. Zhao, Q. Li, G. Li, Y. Du
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Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR

Microbiology Spectrum, 2022
In countries where the incidence of typhoid fever is high, fecal material from short-term carriers of Salmonella Typhi contaminates inadequately treated water supplies.
Ellen E. Higginson, Joseph Nkeze, Jasnehta Permala-Booth, Irene N. Kasumba, Rosanna Lagos, Juan Carlos Hormazabal, Alexander Byrne, Gad Frankel, Myron M. Levine, Sharon M. Tennant   +9 more
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Detection of Pork-Derived Ingredients Based on Real-time Quantitative PCR

Shipin gongye ke-ji, 2022
In order to accurately and reliably quantify pork-derived ingredients in meat products, single-copy gene (carcinoembryonic antigen-related cell adhesion molecule 2-like, CACA) in pig cell nucleus was screened by bioinformatics methods, with CACA gene as ...
Yao YANG, Nengwu SI, Yuao YAN, Rui QIN, Zhihua WU, Li ZHANG   +5 more
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Preliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs

Revista Brasileira de Zootecnia, 2020
To screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets ( Sus scrofa ) with intestinal damage using quantitative real-time PCR (qPCR) and ...
Xiaoyi Qi, Yafei Xu, Weiyun Qin, Haifei Wang, Shenglong Wu, Wenbin Bao   +5 more
doaj   +1 more source