Results 11 to 20 of about 698,289 (197)

Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene

Micromachines, 2020
Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the ...
Junru An, Yangyang Jiang, Bing Shi, Di Wu, Wenming Wu   +4 more
doaj   +1 more source

A quantitative real-time PCR method for absolute telomere length

BioTechniques, 2008
Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our
Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael Fenech   +3 more
doaj   +1 more source

Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra, Chandrashekhar Raut, Neelam Prasad, J S Bhawalkar, Safia Farooqui, Shahzad Mirza, Veshal V Madan   +6 more
doaj   +1 more source

Exogenous Reference RNA for Normalization of Real-Time Quantitative PCR

BioTechniques, 2003
We have utilized an in vitro transcribed 3′ mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were
Reginald D. Smith, Benjamin Brown, Pranvera Ikonomi, Alan N. Schechter   +3 more
doaj   +1 more source

FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

BMC Microbiology, 2012
Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques.
Liu Cindy M, Kachur Sergey, Dwan Michael G, Abraham Alison G, Aziz Maliha, Hsueh Po-Ren, Huang Yu-Tsung, Busch Joseph D, Lamit Louis J, Gehring Catherine A, Keim Paul, Price Lance B   +11 more
doaj   +1 more source

Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi

Microorganisms, 2023
Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH).
Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, Cheng Du   +6 more
doaj   +1 more source

DETERMINAZIONI VIRALI QUANTITATIVE MEDIANTE “PCR REAL TIME” CON IL SISTEMA “ROTOR GENETM”

Microbiologia Medica, 2005
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M.G. Marin, P. Turconi, F. Poma, F. Calamani, P. Guarneri, L. Berardi, L. Lattuada   +6 more
doaj   +1 more source

The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas

Brazilian Journal of Medical and Biological Research, 2009
Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR)
A.C.M.F. Souza, D.R.V. Souza, S.S. Sanabani, R.R. Giorgi, I. Bendit   +4 more
doaj   +1 more source

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR

Frontiers in Cellular and Infection Microbiology, 2022
Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming ...
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su   +7 more
doaj   +1 more source

Development of a droplet digital PCR method for detection of porcine circovirus 4

BMC Veterinary Research, 2023
Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu, Xinru Zhang, Xueying Han, Jiaxing Liu, Lunguang Yao   +4 more
doaj   +1 more source