Results 51 to 60 of about 1,061,192 (206)
Making standards for quantitative real-time pneumococcal PCR [PDF]
Biomolecular Detection and Quantification, 2014 Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2).
Morpeth, Susan C. +3 more
openaire +2 more sources
Investigating the role of DNA methyltransferases in the mantled somaclonal variation of oil palm [PDF]
, 2007 DNA methyltransferase (DMTases) genes have emerged as targets of interest in the exploration of epigenetic mechanisms underlying the mantled variant phenotype in oil palm.
Beulé, Thierry +3 more
core
First detection of Waddlia chondrophila in Africa using SYBR Green real-time PCR on veterinary samples. [PDF]
, 2013 Waddlia chondrophila is a strict intracellular microorganism belonging to the order Chlamydiales that has been isolated twice from aborted bovine fetuses, once in USA and once in Germany. This bacterium is now considered as an abortigenic agent in cattle.
Barkallah, M. +5 more
core +1 more source
The International Journal of Biological Markers, 2006 The assessment of ERα, PgR and HER2 status is routinely performed today to determine the endocrine responsiveness of breast cancer samples. Such determination is usually accomplished by means of immunohistochemistry and in case of HER2 amplification by ...
M. Labuhn +8 more
doaj +1 more source
Quantitative Determination of Lentiviral Vector Particle Numbers by Real-Time PCR
BioTechniques, 2001 Here, we describe a quantitative, DNAbased, real-time PCR approach to determine the number of lentivirus particles that are present in vector preparations.
M. Scherr +4 more
doaj +1 more source
Quantitative real-time PCR for determination of Transgene in Callus of Jatropha curcas [PDF]
, 2014 Jatropha curcas is an important plant belonging to the family Euphorbiaceae which is a potential candidate for biofuel production. Genetic transformation protocol for J.
Jualang Azlan Gansau +3 more
core +1 more source
BMC Genomics, 2022 Background Quantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require normalization with validated reference gene(s).
Jeongah Song +3 more
doaj +1 more source
Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes [PDF]
, 2008 Background Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was
Mihaela Skulj +6 more
core +2 more sources
BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay
BMC Microbiology, 2012 Background Bacterial load quantification is a critical component of bacterial community analysis, but a culture-independent method capable of detecting and quantifying diverse bacteria is needed. Based on our analysis of a diverse collection of 16 S rRNA
Liu Cindy M +6 more
doaj +1 more source