Results 51 to 60 of about 1,315,258 (335)

Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Lycoris aurea

open access: yesFrontiers in Plant Science, 2016
Lycoris aurea (L' Hér.) Herb, a perennial grass species, produces a unique variety of pharmacologically active Amaryllidaceae alkaloids. However, the key enzymes and their expression pattern involved in the biosynthesis of Amaryllidaceae alkaloids ...
Rui Ma   +4 more
semanticscholar   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

open access: yesFEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva   +5 more
wiley   +1 more source

Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species [PDF]

open access: yes, 2016
This study was supported by the National Natural Science Foundation of China, 31471823, the Earmarked Fund for China Agriculture Research System, CARS-35-07, and the Innovation Project of Chinese Academy of Agricultural Science.Studies on grasshopper ...
Cao, Guangchun   +9 more
core   +2 more sources

Quantitative real-time PCR for the clinical detection of Helicobacter pylori

open access: yesGenetics and Molecular Biology, 2007
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human ...
Marcelo Lima Ribeiro   +4 more
doaj   +1 more source

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

open access: yesFEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom   +3 more
wiley   +1 more source

A multiplex quantitative real time PCR to detect Xanthomonas axonopodis pv.allii from onion seeds : [P2-26] [PDF]

open access: yes, 2010
Bacterial blight of onion is a seed-borne emerging disease threatening world onion production, and causing damage to other Allium crops. Its causal agent, #Xanthomonas axonopodis pv.
Escalon, Aline   +3 more
core  

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

open access: yesMolecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero   +8 more
wiley   +1 more source

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

open access: yes, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An   +2 more
core   +1 more source

Quantitative Determination of Lentiviral Vector Particle Numbers by Real-Time PCR

open access: yesBioTechniques, 2001
Here, we describe a quantitative, DNAbased, real-time PCR approach to determine the number of lentivirus particles that are present in vector preparations.
M. Scherr   +4 more
doaj   +1 more source

Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations

open access: yesJournal of Veterinary Research, 2022
Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease.
Souza Camilla de   +6 more
doaj   +1 more source

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