Rapid titer determination using quantitative real-time PCR [PDF]
Gene Therapy, 1999 Quantitative real-time PCR was utilized to evaluate retroviral vector titer. RNA was prepared from vector supernatant and run in a one-step RT-PCR reaction combining reverse transcription (RT) and amplification in one tube. Sample analysis was performed in the ABI Prism 7700 Sequence Detector.
N, Sanburn, K, Cornetta
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Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species [PDF]
, 2016 This study was supported by the National Natural Science Foundation of China, 31471823, the Earmarked Fund for China Agriculture Research System, CARS-35-07, and the Innovation Project of Chinese Academy of Agricultural Science.Studies on grasshopper ...
Cao, Guangchun +9 more
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Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]
, 2019 Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An +2 more
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Journal of Veterinary Research, 2022 Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease.
Souza Camilla de +6 more
doaj +1 more source
RTPrimerDB : the portal for real-time PCR primers and probes [PDF]
, 2009 RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked to genome information from reference databases and ...
S. Lefever +7 more
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PLoS ONE, 2017 Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without the need of ...
Yogita Maheshwari +3 more
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Clinical and Experimental Gastroenterology, 2020 Nabi Jomehzadeh,1– 3 Hazhir Javaherizadeh,4 Mansour Amin,2,3 Mohammad Rashno,1 Ali Teimoori5 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Infectious and Tropical Diseases Research Center ...
Jomehzadeh N +4 more
doaj
Validation of reference genes for gene expression studies in tartary buckwheat (Fagopyrum tataricum Gaertn.) using quantitative real-time PCR [PDF]
PeerJ, 2019 Quantitative real-time reverse transcriptase polymerase chain reaction is a sensitive technique for quantifying gene expression levels. By implementing three distinct algorithms (geNorm, normFinder and BestKeeper), we have validated the stability of the ...
Chenglei Li +10 more
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PCR-based assays for detecting Xanthomonas axonopodis pv. allii in onion seed [PDF]
, 2012 Bacterial blight of onion is an emerging disease threatening world onion production, and causing damage to other Allium crops. The causal agent, Xanthomonas axonopodis pv.
Chabirand, Aude +4 more
core
Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms [PDF]
, 2006 Background Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legislative requirements.
Žel Jana +4 more
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