Results 51 to 60 of about 17,555 (209)
eLife, 2017 Rab GTPases, which are involved in intracellular trafficking pathways, have recently been reported to be ubiquitinated. However, the functions of ubiquitinated Rab proteins remain unexplored. Here we show that Rab5 is monoubiquitinated on K116, K140, and
Donghyuk Shin +7 more
doaj +1 more source
Enrichment of Phosphatidylethanolamine in Viral Replication Compartments via Co-opting the Endosomal Rab5 Small GTPase by a Positive-Strand RNA Virus. [PDF]
PLoS Biology, 2016 Positive-strand RNA viruses build extensive membranous replication compartments to support replication and protect the virus from antiviral responses by the host.
Kai Xu, Peter D Nagy
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Rab5 and Rabankyrin5 overexpression increases macropinosome formation.
, 2013 The distribution of Rab5, Rabankyrin-5 (A) or Rab5(Q79L) (B) in green relative to dextran-positive macropinosomes (red) Scale bar = 10 µm. (C) HEK-Flp-In cells were transiently transfected with pEGFP-C1, pEGFP-Rab5, pEGFP-Rab5-Q79L and pEYFP-Rabankyrin ...
Rohan D. Teasdale (13977) +5 more
core +1 more source
Advanced Science, EarlyView.Amphiphilic sulfonated chitosan nanoparticles buffer endo/lysosomal acidity in microglia by limiting V‐ATPase membrane recruitment and restoring endosomal homeostasis. Following systemic administration, they accumulate in the ischemic hemisphere partly through association with infiltrating immune cells, suppress TLR3/IRF3 and TLR4/NF‐κB signaling ...
Xuanlin Wang +4 more
wiley +1 more source
, 2013 A. By yeast two-hybrid screening, positive clones were identified as Rab5b and Rab5c. Rab5a, subsequently tested, was also able to grow on selective medium and was positive for the β–galactosidase filter assay.
Emilie A. Leclerc (354838) +4 more
core +1 more source
Advanced Science, EarlyView.A novel geometry‐resolved membrane platform provides a seamlessly integrated pipeline for studying curvature‐sensing proteins. By linking shotgun proteomics with quantitative imaging, this approach enables both the unbiased discovery and rigorous validation of novel sensors across the entire curvature range—positive, negative, and zero.
Takumi Komikawa +6 more
wiley +1 more source
Lyn regulate infection-induced autophagy through Rab5.
, 2016 (A) MH-S cells were transfected with Rab5-RFP for 24 h. Cells were infected with PAO1-GFP (MOI = 10, 1 h). Immunostaining was used to detect pLyn. Pa surrounded by Rab5 and pLyn was detected by confocal microscope. Scale bar = 5 μm. (B) The percentage of
Min Yu (120607) +13 more
core +1 more source
Rab5 activation as a tumor cell migration switch [PDF]
Small GTPases, 2014 Increased cell migration is an acquired feature of metastatic cancer cells and relies on derailed signal transduction pathways. Intracellular vesicular trafficking plays a key role in cell migration due to its intricate involvement in cargo transport and membrane composition.
Pablo, Mendoza +3 more
openaire +3 more sources
Advanced Science, EarlyView.Diabetic bone defects remain a critical clinical challenge. A 3D‐printed BCPG‐MF@Y scaffold, embedding Yoda1‐functionalized manganese dioxide‐ferritin nanozymes, scavenges mtROS, suppresses the cGAS–STING pathway, and reverses cellular senescence, while Yoda1 activates Piezo1 to direct BMSCs fate to synergistically reprogram the osteoimmune ...
Qing‐Song Deng +16 more
wiley +1 more source