Results 131 to 140 of about 83,967 (245)

Rac1 mutants interact with CaM-Sepharose.

, 2012
(A) HA-Rac1 WT and HA-Rac1 mutants or (B) GFP-Rac1 wild type, GFP-Rac1(Q61L) and GFP-Rac1(T17N) were expressed in HeLa cells. CaM-Sepharose 4B beads (80 µl) were incubated with HeLa cell lysate (200 μg) expressing various forms of HA-Rac1 or GFP-Rac1 for
Rajinder P. Bhullar (142507), Prashen Chelikani (48039), Bing Xu (69508)   +2 more
core   +1 more source

Circulating RAC1 contributed to steroid-sensitive nephrotic syndrome: Mendelian randomization, single-cell RNA-sequencing, proteomic, and experimental evidence

Renal Failure
Objectives The small GTPase Rac1 (RAC1) has been linked to podocyte disorders and steroid-sensitive nephrotic syndrome (SSNS). The aim of this study was to explore and validate the potential causal association between circulating RAC1 and SSNS.Methods ...
Yi Zhang, Tianwen Yao, Yanqiu Xu, Yi Wang, Shisheng Han   +4 more
doaj   +1 more source

Understanding extracellular vesicle biology, isolation, and characterization: The theory of EV‐erything

Periodontology 2000, EarlyView.
Abstract Background Extracellular vesicles (EVs), especially exosomes, are nanoparticles increasingly recognized as key regulators of intercellular communication in both physiological and pathological aspects. Despite rapid progress, inconsistencies in nomenclature, isolation, and characterization continue to restrict translational advancement.
Paras Ahmad, Nima Farshidfar, Nathan E. Estrin, Yulan Wang, Yufeng Zhang, Anton Sculean, Richard J. Miron   +6 more
wiley   +1 more source

In vitro muscle contraction: A technical review on electrical pulse stimulation in C2C12 cells

Experimental Physiology, EarlyView.
Abstract Electrical pulse stimulation (EPS) of skeletal muscle cells is increasingly used to model exercise In vitro. The murine C2C12 myotube system has become a common platform for such studies, yet wide variability in EPS protocols hampers reproducibility and cross‐study comparisons.
Mark R. C. van de Meene, Anita M. van den Hoek, Roeland Hanemaaijer, Lars Verschuren, Jelle C. B. C. de Jong   +4 more
wiley   +1 more source

New dimensions in CXCR4 and Rac1 regulation [PDF]

, 2013
To gain more insights in the molecular mechanisms regulating cellular migration, which is an important process involved in beneficial biological processes as well as in pathological conditions, we focused our research on two crucial proteins, i,e.
Zoughlami, Y.
core   +15 more sources

An acute crossover trial of passive movement training with and without blood flow restriction to determine the impact on postprandial glycaemia

Experimental Physiology, EarlyView.
Abstract During glucose uptake into muscle, GLUT‐4 can translocate with stimulus from insulin or muscle contraction via exercise, but some exercise‐intolerant populations are unable to benefit from improved glycaemic control. We investigated how passive movement training (PMT) and passive movement training with blood flow restriction (PMT+BFR) could be
Jay Railton‐Sowerby, Ewan Dean, Matthew Davitt, Jack Hayden, Ash Osborne, Donna Shrestha, David Cheneler, Christopher J. Gaffney, Paul Hendrickse   +8 more
wiley   +1 more source

Computational modelling of cardiac fibroblast signalling reveals a key role for Ca2+ in driving atrial fibrillation‐associated fibrosis

The Journal of Physiology, EarlyView.
Abstract figure legend A pre‐existing fibroblast signalling model was enhanced by integrating atrial‐specific components and reactions linked to atrial fibrosis and atrial fibrillation (AF). The analysis highlights the central role of Ca2⁺ signalling in driving profibrotic responses to AF‐relevant stimuli, angiotensin‐II and transforming growth factor ...
Najme Khorasani, Haibo Ni, Jeffrey J Saucerman, Dobromir Dobrev, Stefano Morotti, Eleonora Grandi   +5 more
wiley   +1 more source

Excessive training does not induce mitochondrial dysfunction or impair insulin signalling within skeletal muscle

The Journal of Physiology, EarlyView.
Abstract figure legend Three weeks of overtraining in trained endurance athletes reduced exercise performance without impairing glucose tolerance. In skeletal muscle, overtraining was associated with increased expression of lipid metabolism‐related proteins, enhanced mitochondrial biogenesis and preserved insulin signalling, despite elevated oxidative ...
Geneviève J. DesOrmeaux, Henver S. Brunetta, Pierre‐Andre Barbeau, Alexandra M. Coates, Christopher Pignanelli, Fasih A. Rahman, Jamie F. Burr, Graham P. Holloway   +7 more
wiley   +1 more source

Hypoxia and the cytoskeleton

The Journal of Physiology, EarlyView.
Abstract figure legend Schematic outlining the activation of hypoxia‐sensitive pathways, the influence of hypoxia and associated pathways on the cytoskeleton, and the impact of these on disease progression. Abstract A highly‐regulated and dynamic cytoskeleton is vital for functional cellular physiology and the maintenance of homeostasis.
Darragh Flood, Cormac T. Taylor
wiley   +1 more source

Peripheral regeneration of Aβ low‐threshold mechanoreceptors is limited despite activation of regenerative transcriptional pathways

The Journal of Physiology, EarlyView.
Abstract figure legend The regeneration of Aβ low‐threshold mechanoreceptors (Aβ‐LTMRs), sensory neurons responsible for discriminative touch, has not previously been explored. Here, we took advantage of the selective expression of Calb1 in Aβ‐LTMRs to generate two genetic mouse models for studying their regenerative response after nerve injury.
Sara Bolívar, Natàlia Martínez‐Mateu, David Ovelleiro, Esther Udina   +3 more
wiley   +1 more source