Previously, radioimmunoassay (RIA) has been the only assay to measure insulin-like growth factor-1 (IGF-1) to diagnose hypersomatotropism (HS). Due to radiation concerns, availability, and the cost of IGF-1 RIA, validation of assays for automated ...
Arne Güssow +6 more
doaj +1 more source
Triiodothyronine Radioimmunoassay [PDF]
Highly specific antisera to triiodothyronine (T(3)) were prepared by immunization of rabbits with T(3)-bovine serum albumin conjugates. Antisera with T(3) binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T(3-) (125)I to anti-T(3) serum was found to vary considerably. l-T(3), d-T(3)
J, Lieblich, R D, Utiger
openaire +2 more sources
Phytochrome Radioimmunoassay [PDF]
A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay and requires less than 1 microliter of crude extract from dark-grown plants for quantitation of phytochrome.
R E, Hunt, L H, Pratt
openaire +2 more sources
Validation of Immunoassays for Total Thyroxine and Free Thyroxine and Age and Sex-Related Variation in Thyroid Hormone Serum Concentrations in Orangutans (Pongo sp.). [PDF]
ABSTRACT Background Thyroid disease is reported in orangutans, but to properly diagnose and manage these disorders, reference intervals for circulating thyroid hormones are needed. Methods Commercial immunoassay kits for total thyroxine (TT4) and free thyroxine (FT4) were validated for use in orangutans (Pongo spp.).
Fayette MA +4 more
europepmc +2 more sources
Utilization of Polyspecific Antiserum for Specific Radioimmunoassays: Radioimmunoassays for Rat Fetuin and Bikunin Were Developed by Using Antiserum Against Total Rat Serum Proteins [PDF]
Polyspecific antiserum against total rat serum proteins was used to develop specific and sensitive radioimmunoassays for fetuin and bikunin, two minor protein components of rat plasma. The radioimmunoassays proved to be highly useful to trace bikunin and
Edwin Fink +6 more
core +1 more source
Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay
Method for determining the contents of progesterone in female silkworm (Bombyx mori) moth was studied. The progesterone could be efficiently extracted out from the female silkworm moth according to following conditions: grinding the female moth powder ...
GU Yan-yan, HE Wei-qiang, SHI Lian-gen
doaj +1 more source
Hypocretin Levels in Sporadic and Familial Cases of Canine Narcolepsy
Familial and sporadic forms of narcolepsy exist in both humans and canines. Mutations in the hypocretin receptor 2 gene (Hcrtr 2) cause canine familial narcolepsy.
Beth Ripley +4 more
doaj +1 more source
Development of a Radioimmunoassay for Pig Pancreatic Kallikrein [PDF]
Peer ...
Erhardt F. +6 more
core +2 more sources
Single measurements of salivary and plasmatic oxytocin are used as indicators of the physiology of the oxytocin system. However, questions remain about whether they are sufficiently stable to provide valid trait markers of the physiology of the oxytocin ...
Daniel Martins +3 more
doaj +1 more source
Comparison of four different algorithms for the calculation of radioimmunoassay standard curves [PDF]
Peer ...
Herndl, R. +2 more
core +2 more sources

