Results 51 to 60 of about 226,892 (266)
Rational Design of Improved Pharmabiotics [PDF]
BioMed Research International, 2009 Herein we review the most recent advances in probiotic research and applications with particular emphasis on the novel concept of patho‐biotechnology: the application of pathogen‐derived (ex vivo and in vivo) stress survival strategies for the design of more technologically robust and effective probiotic cultures with improved biotechnological and ...
Sleator, Roy D., Hill, Colin
openaire +2 more sources
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Enhancement of the Thermostability of Microbacterium Esterase by Combinatorial Rational Design
MoleculesThe esterase EstSIT01 from Microbacterium can catalyze the asymmetric hydrolysis of meso-dimethyl ester to produce the crucial chiral intermediate (4S, 5R)-hemimethyl ester for d-biotin synthesis.
Wenyu Peng, Xiaomei Wu, Baodi Ma, Yi Xu
doaj +1 more source
Rational Design and Biotechnological Production of Novel AfpB-PAF26 Chimeric Antifungal Proteins
Microorganisms, 2018 Antimicrobial peptides (AMPs) have been proposed as candidates to develop new antimicrobial compounds for medicine, agriculture, and food preservation.
Marcos Heredero +4 more
doaj +1 more source
The "Divine Design" argument in the English philosophy of nature in the 17th century [PDF]
Вестник Православного Свято-Тихоновского гуманитарного университета: Серия I. Богословие, философия, 2014 The article deals with the development of the teleological argument (the design argument) as elaborated in the works of the English natural philosophers Robert Boyle and John Ray.
Igor Koshelev
doaj +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Dependence of the thickness of laminated load-bearing composite panels on the fiber angles [PDF]
Космические аппараты и технологииThe designing of thin-walled panels made of laminated composites of minimal weight constrained by strength and buckling is difficult and time-consuming because buckling strongly depends on bending stiffness.
Yu. I. Badrukhin, E. S. Terekhova
doaj
FEBS Letters, EarlyView.The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi +6 more
wiley +1 more source