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Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum [PDF]

open access: yesEmerging Infectious Diseases, 2005
To the Editor: An outbreak of rectal lymphogranuloma venereum (LGV) has been detected in the Netherlands among men who have sex with men (1–4). More cases of LGV in other European countries such as Belgium, France, and the United Kingdom have been reported, and the first cases have been detected in the United States as well.
Servaas A. Morré   +5 more
doaj   +6 more sources

Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 [PDF]

open access: yesEmerging Infectious Diseases, 2004
During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated ...
Junhui Zhai   +17 more
doaj   +4 more sources

Rickettsia prowazekii and Real-time Polymerase Chain Reaction

open access: yesEmerging Infectious Diseases, 2006
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka   +4 more
doaj   +3 more sources

Low-Cost, Real-Time Polymerase Chain Reaction System with Integrated RNA Extraction [PDF]

open access: yesSensors, 2023
Rapid, easy-to-use, and low-cost systems for biological sample testing are important for point-of-care diagnostics and various other health applications.
Tchamie Kadja   +2 more
doaj   +2 more sources

Introduction of Digital Polymerase Chain Reaction: Comparison with Real-time Polymerase Chain Reaction

open access: yesKorean Journal of Clinical Laboratory Science
Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj   +2 more sources

REAL TIME POLYMERASE CHAIN REACTION IN TULAREMIA LABORATORY DIAGNOSTICS

open access: yesЖурнал микробиологии, эпидемиологии и иммунобиологии, 2015
Aim. Enhancement of tularemia laboratory diagnostics by F. tularensis DNA determination in blood sera of patients using real time polymerase chain reaction (RT-PCR). Materials and methods.
M. I Kormilitsyna   +3 more
doaj   +2 more sources

Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]

open access: yesArchives of Razi Institute, 2020
Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
doaj   +1 more source

Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera [PDF]

open access: yesVeterinary World, 2021
Background and Aim: Different polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of Brucella DNA in serum samples of different animal species and humans without being validated or properly validated ...
Nour H. Abdel-Hamid   +5 more
doaj   +1 more source

Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 Patients

open access: yesMicrobiology Spectrum, 2022
Coronavirus disease 2019 (COVID-19) is a mild to severe respiratory illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Merlin Jayalal Lawrence Panchali   +8 more
doaj   +1 more source

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