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Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum [PDF]
To the Editor: An outbreak of rectal lymphogranuloma venereum (LGV) has been detected in the Netherlands among men who have sex with men (1–4). More cases of LGV in other European countries such as Belgium, France, and the United Kingdom have been reported, and the first cases have been detected in the United States as well.
Servaas A. Morré+5 more
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Rickettsia prowazekii and Real-time Polymerase Chain Reaction [PDF]
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka+4 more
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Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction [PDF]
Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful data from this method depend on fitting data to theoretical curves that allow computation of mRNA levels.
Sheng Zhao, Russell D. Fernald
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Real-Time Polymerase Chain Reaction: Applications in Diagnostic Microbiology
The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. Real-Time PCR (RT-PCR) is becoming the gold standard test for accurate, sensitive and fast diagnosis for a large range of infectious agents.
Kordo B.A. Saeed, Nusreen S. Ahmad
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Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
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Trends and advances in food analysis by real-time polymerase chain reaction [PDF]
Analyses to ensure food safety and quality are more relevant now because of rapid changes in the quantity, diversity and mobility of food. Food-contamination must be determined to maintain health and up-hold laws, as well as for ethical and cultural concerns.
Minhaz Uddin Ahmed+3 more
openaire +4 more sources
Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 Patients
Coronavirus disease 2019 (COVID-19) is a mild to severe respiratory illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Merlin Jayalal Lawrence Panchali+8 more
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Low-Cost, Real-Time Polymerase Chain Reaction System with Integrated RNA Extraction [PDF]
Rapid, easy-to-use, and low-cost systems for biological sample testing are important for point-of-care diagnostics and various other health applications.
Tchamie Kadja+2 more
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Real-time polymerase chain reaction in recurrent cytomegalovirus anterior uveitis
Purpose: Cytomegalovirus (CMV) has been reported to cause anterior uveitis in the immunocompetent people. Recurrence of this viral uveitis poses a mangement dilemma.
Vinita Girish Rao+2 more
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Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko+3 more
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