Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum [PDF]
To the Editor: An outbreak of rectal lymphogranuloma venereum (LGV) has been detected in the Netherlands among men who have sex with men (1–4). More cases of LGV in other European countries such as Belgium, France, and the United Kingdom have been reported, and the first cases have been detected in the United States as well.
Servaas A. Morré +5 more
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Rickettsia prowazekii and Real-time Polymerase Chain Reaction
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka +4 more
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Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
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REAL TIME POLYMERASE CHAIN REACTION IN TULAREMIA LABORATORY DIAGNOSTICS
Aim. Enhancement of tularemia laboratory diagnostics by F. tularensis DNA determination in blood sera of patients using real time polymerase chain reaction (RT-PCR). Materials and methods.
M. I Kormilitsyna +3 more
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Real-Time Polymerase Chain Reaction: Feed Two Birds with One Seed? [PDF]
Yoon Jin Choi
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Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
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Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera [PDF]
Background and Aim: Different polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of Brucella DNA in serum samples of different animal species and humans without being validated or properly validated ...
Nour H. Abdel-Hamid +5 more
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Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 Patients
Coronavirus disease 2019 (COVID-19) is a mild to severe respiratory illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Merlin Jayalal Lawrence Panchali +8 more
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Clinical epidemiological applicability of real-time polymerase chain reaction for COVID-19 [PDF]
Objectives Real-time polymerase chain reaction is currently used as a confirmatory test for coronavirus disease 2019 (COVID-19). The test results are interpreted as positive, negative, or inconclusive, and are used only for a qualitative classification ...
Geehyuk Kim +4 more
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Real-Time Polymerase Chain Reaction: Applications in Diagnostic Microbiology
The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. Real-Time PCR (RT-PCR) is becoming the gold standard test for accurate, sensitive and fast diagnosis for a large range of infectious agents.
Kordo B.A. Saeed, Nusreen S. Ahmad
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