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Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum [PDF]

open access: yesEmerging Infectious Diseases, 2005
To the Editor: An outbreak of rectal lymphogranuloma venereum (LGV) has been detected in the Netherlands among men who have sex with men (1–4). More cases of LGV in other European countries such as Belgium, France, and the United Kingdom have been reported, and the first cases have been detected in the United States as well.
Servaas A. Morré   +5 more
doaj   +14 more sources

Rickettsia prowazekii and Real-time Polymerase Chain Reaction [PDF]

open access: yesEmerging Infectious Diseases, 2006
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka   +4 more
doaj   +5 more sources

Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction [PDF]

open access: yesJournal of Computational Biology, 2005
Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful data from this method depend on fitting data to theoretical curves that allow computation of mRNA levels.
Sheng Zhao, Russell D. Fernald
openaire   +4 more sources

Real-Time Polymerase Chain Reaction: Applications in Diagnostic Microbiology

open access: yesInternational Journal of Medical Students, 2013
The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. Real-Time PCR (RT-PCR) is becoming the gold standard test for accurate, sensitive and fast diagnosis for a large range of infectious agents.
Kordo B.A. Saeed, Nusreen S. Ahmad
doaj   +5 more sources

Introduction of Digital Polymerase Chain Reaction: Comparison with Real-time Polymerase Chain Reaction

open access: yesKorean Journal of Clinical Laboratory Science
Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj   +2 more sources

Trends and advances in food analysis by real-time polymerase chain reaction [PDF]

open access: yesJournal of Food Science and Technology, 2016
Analyses to ensure food safety and quality are more relevant now because of rapid changes in the quantity, diversity and mobility of food. Food-contamination must be determined to maintain health and up-hold laws, as well as for ethical and cultural concerns.
Minhaz Uddin Ahmed   +3 more
openaire   +4 more sources

Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 Patients

open access: yesMicrobiology Spectrum, 2022
Coronavirus disease 2019 (COVID-19) is a mild to severe respiratory illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Merlin Jayalal Lawrence Panchali   +8 more
doaj   +4 more sources

Low-Cost, Real-Time Polymerase Chain Reaction System with Integrated RNA Extraction [PDF]

open access: yesSensors, 2023
Rapid, easy-to-use, and low-cost systems for biological sample testing are important for point-of-care diagnostics and various other health applications.
Tchamie Kadja   +2 more
doaj   +2 more sources

Real-time polymerase chain reaction in recurrent cytomegalovirus anterior uveitis

open access: yesAmerican Journal of Ophthalmology Case Reports, 2019
Purpose: Cytomegalovirus (CMV) has been reported to cause anterior uveitis in the immunocompetent people. Recurrence of this viral uveitis poses a mangement dilemma.
Vinita Girish Rao   +2 more
doaj   +4 more sources

Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction

open access: yesВопросы вирусологии, 2020
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko   +3 more
doaj   +5 more sources

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