Clinical epidemiological applicability of real-time polymerase chain reaction for COVID-19 [PDF]
Osong Public Health and Research Perspectives, 2022 Objectives Real-time polymerase chain reaction is currently used as a confirmatory test for coronavirus disease 2019 (COVID-19). The test results are interpreted as positive, negative, or inconclusive, and are used only for a qualitative classification ...
Geehyuk Kim +4 more
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Real-Time Polymerase Chain Reaction: Applications in Diagnostic Microbiology
International Journal of Medical Students, 2013 The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. Real-Time PCR (RT-PCR) is becoming the gold standard test for accurate, sensitive and fast diagnosis for a large range of infectious agents.
Kordo B.A. Saeed, Nusreen S. Ahmad
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On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]
, 2007 We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
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A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test [PDF]
, 2014 A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species.
De Craeye, Stéphane +6 more
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Revista Panamericana de Salud Pública, 2022 Objective. To evaluate molecular tools to detect low-level parasitemia and the five species of Plasmodium that infect humans for use in control and elimination programs, and in reference laboratories. Methods. We evaluated 145 blood samples from patients
Mariana Aschar +8 more
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Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients [PDF]
, 2013 OBJECTIVE: This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients.
Bellei, Nancy Cristina Junqueira +5 more
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Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
Taiwanese Journal of Obstetrics & Gynecology, 2009 Objective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation ...
Horng-Jyh Tsai, MD, PhD, FACOG
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Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]
, 2011 Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els +3 more
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Influenza a H5N1 detection [PDF]
, 2005 We developed a sensitive and rapid real-time reverse transcription- polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples.
Cheng, PKC +4 more
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Real-time polymerase chain reaction in recurrent cytomegalovirus anterior uveitis
American Journal of Ophthalmology Case Reports, 2019 Purpose: Cytomegalovirus (CMV) has been reported to cause anterior uveitis in the immunocompetent people. Recurrence of this viral uveitis poses a mangement dilemma.
Vinita Girish Rao +2 more
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