Results 21 to 30 of about 734,685 (383)

Ultrafast and Real-Time Nanoplasmonic On-Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics.

ACS Nano, 2021
Advent and fast spread of pandemic diseases draw worldwide attention to rapid, prompt, and accurate molecular diagnostics with technical development of ultrafast polymerase chain reaction (PCR). Microfluidic on-chip PCR platforms provide highly efficient
Byoung-Hoon Kang, Youngseop Lee, E. Yu, Hamin Na, Minhee Kang, H. Huh, K. Jeong   +6 more
semanticscholar   +1 more source

Ultrasensitive molecular tests for Plasmodium detection: applicability in control and elimination programs and reference laboratories

Revista Panamericana de Salud Pública, 2022
Objective. To evaluate molecular tools to detect low-level parasitemia and the five species of Plasmodium that infect humans for use in control and elimination programs, and in reference laboratories. Methods. We evaluated 145 blood samples from patients
Mariana Aschar, Maria Carmen A. Sanchez, Maria de Jesus Costa-Nascimento, Maria de Lourdes R. N. Farinas, Angélica D. Hristov, Giselle F. M. C. Lima, Juliana Inoue, José E. Levi, Silvia M. Di Santi   +8 more
doaj   +1 more source

On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]

, 2007
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction

Taiwanese Journal of Obstetrics & Gynecology, 2009
Objective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation ...
Horng-Jyh Tsai, MD, PhD, FACOG
doaj   +3 more sources

False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

Korean Journal of Radiology, 2020
The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide.
Dasheng Li, Dawei Wang, Jianping Dong, Nan Wang, He Huang, Haiwang Xu, Chen Xia   +6 more
semanticscholar   +1 more source

Comparison of 2 new real‐time polymerase chain reaction–based urinary markers in the follow‐up of patients with non–muscle‐invasive bladder cancer

Cancer Cytopathology, 2020
The objective of the current study was to compare the diagnostic accuracy of 2 new real‐time polymerase chain reaction–based urinary markers with each other and with urinary cytology, cystoscopy, and/or histology in patients being followed for non–muscle‐
E. Trenti, S. Pycha, C. Mian, C. Schwienbacher, E. Hanspeter, M. Kafka, G. Spedicato, E. Vjaters, S. Degener, A. Pycha, C. D’Elia   +10 more
semanticscholar   +1 more source

Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients [PDF]

, 2013
OBJECTIVE: This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients.
Bellei, Nancy Cristina Junqueira, Granato, Celso Francisco Hernandes, Guatura, Sandra Baltazar, Perosa, Ana Helena Sitta, Silva, Ellen Ricci Monteiro da, Watanabe, Aripuanã Sakurada Aranha   +5 more
core   +4 more sources

Influenza a H5N1 detection [PDF]

, 2005
We developed a sensitive and rapid real-time reverse transcription- polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples.
Cheng, PKC, Hoang, TL, Lim, WWL, Ng, AYY, Ng, EKO   +4 more
core   +1 more source

Identification of Cronobacter in infant formula using real-time polymerase chain reaction

Zhongguo shipin weisheng zazhi, 2023
ObjectiveTo establish a real-time quantitative polymerase chain reaction （PCR） method for rapid and accurate identification of Cronobacter spp. in food samples and artificially contaminated samples.MethodsPrimers and probes were designed based on the ...
WANG Qinglong, ZHANG Yuechuan, ZHOU Yanxia, GONG Youbo, LI Shuang, SHI Jinshuo, DING Shanshan, RAN Linglei, WANG Kaiyi, TANG Yuhan, CAI Xuefeng   +10 more
doaj   +1 more source

DETECTION OF CAMPYLOBACTER SPP. WITH REAL-TIME POLYMERASE CHAIN REACTION

Ветеринария сегодня, 2019
Bacteria of Campylobacter genus are ones of the main zoonotic pathogens causing human and animal diseases. Campylobacter organisms are microaerophiles and, therefore, require low oxygen concentration (3–5%) and high carbon dioxide concentration (3–10 ...
G. S. Skitovich, K. V. Serova, N. B. Shadrova, O. V. Pruntova   +3 more
doaj   +1 more source