Results 21 to 30 of about 637,530 (302)

Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma [PDF]

open access: yes, 2015
Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy.
Asadi, J.   +9 more
core   +1 more source

DETECTION OF CAMPYLOBACTER SPP. WITH REAL-TIME POLYMERASE CHAIN REACTION

open access: yesВетеринария сегодня, 2019
Bacteria of Campylobacter genus are ones of the main zoonotic pathogens causing human and animal diseases. Campylobacter organisms are microaerophiles and, therefore, require low oxygen concentration (3–5%) and high carbon dioxide concentration (3–10 ...
G. S. Skitovich   +3 more
doaj   +1 more source

Identification of Cronobacter in infant formula using real-time polymerase chain reaction

open access: yesZhongguo shipin weisheng zazhi, 2023
ObjectiveTo establish a real-time quantitative polymerase chain reaction (PCR) method for rapid and accurate identification of Cronobacter spp. in food samples and artificially contaminated samples.MethodsPrimers and probes were designed based on the ...
WANG Qinglong   +10 more
doaj   +1 more source

Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2018
Introduction: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum.
Rubén E. Varela-M   +2 more
doaj   +1 more source

The impact of co-infection of influenza A virus on the severity of Middle East Respiratory Syndrome Coronavirus [PDF]

open access: yes, 2017
This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Al-Tawfiq, Jaffar A.   +4 more
core   +1 more source

Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction [PDF]

open access: yes, 2005
Aim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.
Chen, Y   +9 more
core   +1 more source

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction [PDF]

open access: yes, 2012
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively.
Ballhause, M.   +16 more
core   +5 more sources

ML Estimation of DNA Initial Copy Number in Polymerase Chain Reaction (PCR) Processes [PDF]

open access: yes, 2007
Estimation of DNA copy number in a given biological sample is an extremely important problem in genomics. This problem is especially challenging when the number of the DNA strands is minuscule, which is often the case in applications such as pathogen and
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Introduction of Digital Polymerase Chain Reaction: Comparison with Real-time Polymerase Chain Reaction

open access: yesKorean Journal of Clinical Laboratory Science
Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj   +1 more source

Real-time polymerase chain reaction in acute retinal necrosis following encephalitis

open access: yesIndian Journal of Ophthalmology, 2018
Acute retinal necrosis (ARN) following herpes simplex encephalitis (HSE) in an immunocompetent patient is a rare condition. Quantitative real-time polymerase chain reaction (qPCR) has made it possible to identify and quantify viral genome.
Vinita Rao   +2 more
doaj   +1 more source

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