Results 31 to 40 of about 644,089 (298)

Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction [PDF]

open access: yesJournal of Computational Biology, 2005
Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful data from this method depend on fitting data to theoretical curves that allow computation of mRNA levels.
Sheng, Zhao, Russell D, Fernald
openaire   +2 more sources

Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives [PDF]

open access: yes, 2015
This is a final draft version of the publication following acceptance by the journal.Quantitative polymerase chain reaction (qPCR) is a method of amplifying and detecting small samples of genetic material in real time and is in routine use across many ...
Karteris, E, Rogers-Broadway, KR
core   +1 more source

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains [PDF]

open access: yesArchives of Razi Institute, 2019
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents.
S. Alamian   +4 more
doaj   +1 more source

Performance and Cost-effectiveness of Pooling COVID-19 Samples Using Real time Polymerase Chain Reaction Test

open access: yesJournal of Nepal Health Research Council, 2022
Background: Rapid detection of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) by real-time polymerase chain reaction (RT-PCR) is the most reliable method used worldwide.
Ira Shrestha   +5 more
doaj   +1 more source

Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia

open access: yesRevista Brasileira de Hematologia e Hemoterapia, 2015
Introduction: Minimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia.
Francisco Danilo Ferreira Paula   +7 more
doaj   +1 more source

Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction

open access: yesВопросы вирусологии, 2020
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko   +3 more
doaj   +1 more source

Real time polymerase chain reaction for the quantitative detection [PDF]

open access: yes, 2009
Soybean protein is reported to be the most widely used vegetable protein in the meat industry. Several characteristics of soybean protein such as the emulsifier properties, preventing the coalescence of fat during heating, and the increased capacity of ...
Amaral, Joana S.   +3 more
core  

Interleukin-21 receptor might be a novel therapeutic target for the treatment of rheumatoid arthritis [PDF]

open access: yes, 2014
Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by the synovial inflammation of the joints. Various cells and cytokines have been identified that may contribute to RA pathology. Interleukin (IL)-21 is a proinflammatory
Andersson   +50 more
core   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

open access: yesFEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

open access: yesFEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva   +5 more
wiley   +1 more source

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