Journal of Molecular Endocrinology, 2000 The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples.
S. Bustin
semanticscholar +1 more source
Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]
, 2011 Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els +3 more
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Quantification of apolipoprotein E receptors in human brain-derived cell lines by real-time polymerase chain reaction [PDF]
, 2004 Apolipoprotein (apo) E4 is a risk factor for Alzheimer's disease (AD) and other neurodegenerative diseases, compared to wild-type apoE3. The mechanism(s) is unknown.
Dingwall, C +4 more
core +1 more source
Biomédica: revista del Instituto Nacional de Salud, 2018 Introduction: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum.
Rubén E. Varela-M +2 more
doaj +1 more source
Rapid and accurate diagnosis of infection by real-time polymerase chain reaction [PDF]
Intestinal Research, 2018 Background/AimsThe incidence and severity of Clostridium difficile infection (CDI) have increased worldwide, resulting in a need for rapid and accurate diagnostic methods.MethodsA retrospective study was conducted to compare CDI diagnosis methods between
Pil Hun Song +9 more
doaj +1 more source
Real-Time Polymerase Chain Reaction Detection of Fishmeal in Feedstuffs [PDF]
Journal of AOAC INTERNATIONAL, 2010 Abstract A SYBR<sup/> Green PCR system was developed for detection of fishmeal in feedstuffs. The real-time PCR method combines the use of fish-specific primers that amplify an 87 base pair (bp) fragment of the mitochondrial 12S ribosomal RNA gene from fish species, and a positive control primer pair that amplifies a 99 bp ...
Miguel Ángel Pavón +7 more
openaire +3 more sources
Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction [PDF]
, 2005 Aim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.
Chen, Y +9 more
core +1 more source
A kinetic model of quantitative real‐time polymerase chain reaction [PDF]
Biotechnology and Bioengineering, 2005 AbstractReal‐time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined.
Wei Shou Hu, Sarika Mehra
openaire +3 more sources
Cost-Effective Trap qPCR Approach to Evaluate Telomerase Activity: an Important Tool for Aging, Cancer, and Chronic Disease Research [PDF]
Clinics, 2021 OBJECTIVES: Telomeres are a terminal “DNA cap” that prevent chromosomal fusion and degradation. However, aging is inherent to life, and so is the loss of terminal sequences.
Thalyta Nery Carvalho Pinto +4 more
doaj +1 more source
Deteksi Cepat Foodborne Pathogen Bakteri Cronobacter sakazakii pada Susu Bubuk Formula dengan Metode Real-Time Polymerase Chain Reaction [PDF]
, 2023 Abstrak Penyakit penyebab keracunan pangan atau foodborne disease masih menjadi persoalan serius yang harus dihadapi oleh masyarakat dunia. Salah satu penyebab foodborne disease adalah kontaminasi mikroorganisme patogen pada bahan pangan.
Nurjayadi, Muktiningsih +2 more
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